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Phosphotyrosine Antibody (03-7720) in ELISA
Direct ELISA analysis of Phosphotyrosine was performed by coating wells of a 96-well plate with 100 µL per well of Phosphotyrosine or Phosphoserine diluted in carbonate/bicarbonate buffer (Product # 28382) at a concentration of 5 µg/mL overnight at 4C. Wells of the plate were washed, blocked with StartingBlock blocking buffer (Product # 37538), and incubated with 100 µL per well of a HRP-conjugated anti-phosphotyrosine mouse monoclonal antibody (Product # 03-7720) at a starting concentration of 1 µg/mL and serially diluting 2-fold to a con... View More
产品信息
03-7720
应用
建议稀释比
已发表文章
产品规格
种属反应
Chemical
已发表种属
Chemical
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
pY20
抗原
Keyhole limpet hemocyanin coupled with equal amounts of phosphotyrosine, glycine, and alanine.
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
purified
保存液
PBS, pH 7.4, with 40% glycerol, 4mg/mL BSA
内含物
0.1% ProClin 300
保存条件
4° C
运输条件
Wet ice
RRID
AB_2532960
产品详细信息
03-7720 has been successfully used in ELISA and WB applications, and detects proteins which contain phosphorylated tyrosine residues and proteins containing phosphorylated threonine residues.
靶标信息
The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Phosphotyrosine; pTyr; pY
