Invitrogen

CD4 Monoclonal Antibody (OX35), FITC, eBioscience™

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CD4 Antibody (11-0040-82) in Flow

Staining of rat splenocytes with Anti-Rat CD3 PE (Product # 12-0030-82) and 0.125 µg of Mouse IgG2a K Isotype Control FITC (Product # 11-4724-42) (left) or 0.125 µg of Anti-Rat CD4 FITC (right). Cells in the lymphocyte gate were used for analysis.

CD4 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

CD4 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

CD4 Antibody — Figure 3. Combined treatment with simvastatin and rapamycin decreased the levels of CD4 + T cells in the spleens of rats. Flow cytometry was used to analyze the levels of CD4 + T cells in the spleens on (A) day 3, (B) day 5 and (C) day 7 post cardiac transplantation (n=4). **P<0.01 and ***P<0.001. {TM}

产品信息

11-0040-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

0.25 µg/test

查看38篇已发表文章 38篇已发表文章

产品规格

种属反应

Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

OX35

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_953577

产品详细信息

Description: The OX35 monoclonal antibody reacts with rat CD4. CD4 is a co-receptor which participates in signaling through the T cell receptor by making contacts with MHC class II expressed on antigen-presenting cells (APC). CD4 is expressed in the thymus by CD4+CD8+ "double-positive" and CD4+ "single-positive" thymocytes, and by CD4+ "helper" T cells in the periphery. CD4 is a cell-surface membrane glycoprotein which contains four extracellular immunoglobulin-like domains.

Applications Reported: This OX35 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This OX35 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Fri Dec 16 00:00:00 UTC 2022

miR-21 Regulates Immune Balance Mediated by Th17/Treg in Peripheral Blood of Septic Rats during the Early Phase through Apoptosis Pathway.

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Fri Jan 01 00:00:00 UTC 2021

Docosahexaenoic acid activates the Nrf2 signaling pathway to alleviate impairment of spleen cellular immunity in intrauterine growth restricted rat pups.

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Emodin inhibits the progression of acute pancreatitis via regulation of lncRNA TUG1 and exosomal lncRNA TUG1.

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Emodin inhibits the progression of acute pancreatitis via regulation of lncRNA TUG1 and exosomal lncRNA TUG1.

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An inducible rodent glaucoma model that exhibits gradual sustained increase in intraocular pressure with distinct inner retina and optic nerve inflammation.

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Genetic inactivation of RIP1 kinase activity in rats protects against ischemic brain injury.

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Rodent Models of Spondyloarthritis Have Decreased White and Bone Marrow Adipose Tissue Depots.

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Exploring the Mechanism on the Medullary Visceral Zone Inhibiting the Cholinergic Anti-inflammatory Pathway Induced by Sepsis.

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Mon Jul 19 00:00:00 UTC 2021

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Orthodontic treatment induces Th17/Treg cells to regulate tooth movement in rats with periodontitis.

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Experimental and therapeutic medicine

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Experimental and therapeutic medicine

Tu Z,Xue H,Chen W,Cao L,Zhang W

11-0040-82 was used in Flow Cytometry to suggest that Treg and Th17 cells as well as their cytokines may be involved in the pathogenesis of acute bronchitis.

Sun Oct 01 00:00:00 UTC 2017

The effects of freeze-dried Ganoderma lucidum mycelia on a recurrent oral ulceration rat model.

BMC complementary and alternative medicine

Xie L,Zhong X,Liu D,Liu L,Xia Z

11-0040 was used in Flow cytometry/Cell sorting to show that freeze-dried powder from G. lucidum mycelia could potentially be used to treat recurrent oral ulceration.

Fri Dec 01 00:00:00 UTC 2017

The effects of freeze-dried Ganoderma lucidum mycelia on a recurrent oral ulceration rat model.

BMC complementary and alternative medicine

Xie L,Zhong X,Liu D,Liu L,Xia Z

11-0040 was used in Flow cytometry/Cell sorting to show that freeze-dried powder from G. lucidum mycelia could potentially be used to treat recurrent oral ulceration.

Fri Dec 01 00:00:00 UTC 2017

Immunomodulatory effects of Longdan Xiegan Tang on CD4+/CD8+ T cells and associated inflammatory cytokines in rats with experimental autoimmune uveitis.

Molecular medicine reports

Tang K,Guo D,Zhang L,Guo J,Zheng F,Si J,Bi H

11-0040 was used in Flow cytometry/Cell sorting to study the mechanisms that mediate the actions of Longdan Xiegan Tang.

Thu Sep 01 00:00:00 UTC 2016

Deficits in endogenous adenosine formation by ecto-5'-nucleotidase/CD73 impair neuromuscular transmission and immune competence in experimental autoimmune myasthenia gravis.

Mediators of inflammation

Oliveira L,Correia A,Cristina Costa A,Guerra-Gomes S,Ferreirinha F,Magalhães-Cardoso MT,Vilanova M,Correia-de-Sá P

11-0040 was used in Flow cytometry/Cell sorting to investigate the role of ecto-5'-nucleotidase/CD73 in a rat model of experimental autoimmune myasthenia gravis.