Invitrogen

SIGLEC H Monoclonal Antibody (eBio440c), FITC, eBioscience™

SIGLEC H Antibody in Flow Cytometry (Flow)

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SIGLEC H Antibody (11-0333-82) in Flow

Staining of SJL splenocytes with Anti-Human/Mouse CD45R (B220) PE (Product # 12-0452-82) (left), Anti-Mouse CD317 (BST2, PDCA-1) APC (right) and 0.125 µg of Anti-Mouse Siglec H FITC. Cells in the large forward scatter population were used for analysis.

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产品信息

11-0333-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.25 µg/test

查看11篇已发表文章 11篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

eBio440c

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_837163

产品详细信息

Description: The monoclonal antibody eBio440c recognizes Siglec-H, a protein exclusively found on pDC (plasmacytoid dendritic cells) or type I IFN-producing cells (IPC) in the naive mouse. Mouse IPC are typically PDCA+, CD11c+, CD11b-, B220+, and Ly-6C+, and are quick to respond to viruses. Siglec-H is a transmembrane protein of the Ig superfamily that like CD33 have been shown to bind sialic acid but lacks the characteristic cytoplasmic ITIM domain (immunoreceptor tyrosine based inhibitory motif). To overcome the lack of a cytoplasmic domain, Siglec-H associates with DAP12 thereby allowing for signal transduction.

The eBio440c antibody has been shown to inhibit pDC function (inhbits IFNalpha secretion in response to CpG).

It has been observed that some mouse strains (such as SJL) have higher percentages of pDCs compared to C57BL/6.

Applications Reported: This eBio440c antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBio440c antibody has been tested by flow cytometric analysis of SJL mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Siglec-H, a protein exclusively found on pDC (plasmacytoid dendritic cells) or type I IFN-producing cells (IPC) in the naive mouse. Mouse IPC are typically PDCA+, CD11c+, CD11b-, B220+, and Ly-6C+, and are quick to respond to viruses. Siglec-H is a transmembrane protein of the Ig superfamily that like CD33 have been shown to bind sialic acid but lacks the characteristic cytoplasmic ITIM domain (immunoreceptor tyrosine based inhibitory motif). To overcome the lack of a cytoplasmic domain, Siglec-H associates with DAP12 thereby allowing for signal transduction. The eBio440c antibody has been shown to inhibit pDC function (inhbits IFNalpha secretion in response to CpG). It has been observed that some mouse strains (such as SJL) have higher percentages of pDCs compared to C57BL/6.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

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Product References

A lncRNA identifies Irf8 enhancer element in negative feedback control of dendritic cell differentiation.

eLife

Xu H,Li Z,Kuo CC,Götz K,Look T,de Toledo MAS,Seré K,Costa IG,Zenke M

Published figure using SIGLEC H monoclonal antibody (Product # 11-0333-82) in Flow Cytometry

Tue Mar 14 00:00:00 UTC 2023

Characterization of Resident Corneal Plasmacytoid Dendritic Cells and Their Pivotal Role in Herpes Simplex Keratitis.

Cell reports

Jamali A,Hu K,Sendra VG,Blanco T,Lopez MJ,Ortiz G,Qazi Y,Zheng L,Turhan A,Harris DL,Hamrah P

11-0333 was used in Flow cytometry/Cell sorting to report that pDCs constitutively populate naïve corneas and are increased during sterile injuries or acute herpes simplex virus 1 (HSV-1) keratitis.

Tue Sep 01 00:00:00 UTC 2020

Characterization of Resident Corneal Plasmacytoid Dendritic Cells and Their Pivotal Role in Herpes Simplex Keratitis.

Cell reports

Jamali A,Hu K,Sendra VG,Blanco T,Lopez MJ,Ortiz G,Qazi Y,Zheng L,Turhan A,Harris DL,Hamrah P

11-0333 was used in Flow cytometry/Cell sorting to report that pDCs constitutively populate naïve corneas and are increased during sterile injuries or acute herpes simplex virus 1 (HSV-1) keratitis.

Tue Sep 01 00:00:00 UTC 2020

Characterization of immune cell subtypes in three commonly used mouse strains reveals gender and strain-specific variations.

Laboratory investigation; a journal of technical methods and pathology

Hensel JA,Khattar V,Ashton R,Ponnazhagan S

11-0333-82 was used in Flow Cytometry to performed immunophenotypic analysis of bone marrow cells and splenocytes from both genders of three commonly used murine strains (C57BL/6NCr, 129/SvHsd, and BALB/cAnNCr).

Tue Jan 01 00:00:00 UTC 2019

Characterization of immune cell subtypes in three commonly used mouse strains reveals gender and strain-specific variations.

Laboratory investigation; a journal of technical methods and pathology

Hensel JA,Khattar V,Ashton R,Ponnazhagan S

Tue Jan 01 00:00:00 UTC 2019

pDC Activation by TLR7/8 Ligand CL097 Compared to TLR7 Ligand IMQ or TLR9 Ligand CpG.

Journal of immunology research

Wu J,Li S,Li T,Lv X,Zhang M,Zang G,Qi C,Liu YJ,Xu L,Chen J

11-0333-82 was used in Flow cytometry/Cell sorting to aid in the development of a new strategy for future tumor immunotherapy.

Wed Aug 07 00:00:00 UTC 2019

DC subset-specific induction of T cell responses upon antigen uptake via Fcγ receptors in vivo.

The Journal of experimental medicine

Lehmann CHK,Baranska A,Heidkamp GF,Heger L,Neubert K,Lühr JJ,Hoffmann A,Reimer KC,Brückner C,Beck S,Seeling M,Kießling M,Soulat D,Krug AB,Ravetch JV,Leusen JHW,Nimmerjahn F,Dudziak D

11-0333 was used in Flow cytometry/Cell sorting to suggest that targeting antigens to FcγRs on DCs may be a promising approach to raise immunogenic or tolerogenic T cell responses to antigens of choice.

Mon May 01 00:00:00 UTC 2017

Innate lymphoid cells contribute to allergic airway disease exacerbation by obesity.

The Journal of allergy and clinical immunology

Everaere L,Ait-Yahia S,Molendi-Coste O,Vorng H,Quemener S,LeVu P,Fleury S,Bouchaert E,Fan Y,Duez C,de Nadai P,Staels B,Dombrowicz D,Tsicopoulos A

11-0333 was used in Flow cytometry/Cell sorting to indicate that high-fat diet-induced obesity might exacerbate allergic airway inflammation through mechanisms involving ILC2s and ILC3s.

Tue Nov 01 00:00:00 UTC 2016

Canonical and noncanonical Wnt proteins program dendritic cell responses for tolerance.

Journal of immunology (Baltimore, Md. : 1950)

Oderup C,LaJevic M,Butcher EC

11-0333 was used in Flow cytometry/Cell sorting to investigate the role of canonical and noncanonical Wnts in immune tolerance, showing that they program dendritic cell responses for tolerance.

Sat Jun 15 00:00:00 UTC 2013

Plasmacytoid dendritic cells transport peripheral antigens to the thymus to promote central tolerance.

Immunity

Hadeiba H,Lahl K,Edalati A,Oderup C,Habtezion A,Pachynski R,Nguyen L,Ghodsi A,Adler S,Butcher EC

11-0333 was used in Flow cytometry/Cell sorting to explore the mechanisms underlying the contribution to immune tolerance conducted by peripheral dendritic cells.

Fri Mar 23 00:00:00 UTC 2012

CD8α⁺β⁻ and CD8α⁺β⁺ plasmacytoid dendritic cells induce Foxp3⁺ regulatory T cells and prevent the induction of airway hyper-reactivity.

Mucosal immunology

Lombardi V,Speak AO,Kerzerho J,Szely N,Akbari O

11-0333 was used in Flow cytometry/Cell sorting to reveal three functional and phenotypically distinct populations of mice plasmacytoid dendritic cells.

Sun Jul 01 00:00:00 UTC 2012

Plasmacytoid dendritic cells sense skin injury and promote wound healing through type I interferons.

The Journal of experimental medicine

Gregorio J,Meller S,Conrad C,Di Nardo A,Homey B,Lauerma A,Arai N,Gallo RL,Digiovanni J,Gilliet M

11-0333 was used in Flow cytometry/Cell sorting to uncover a new role of plasmacytoid dendritic cells in sensing tissue damage and promoting wound repair at skin surfaces.

Mon Dec 20 00:00:00 UTC 2010

CD4 T-cell suppression by cells from Toxoplasma gondii-infected retinas is mediated by surface protein PD-L1.

Infection and immunity

Charles E,Joshi S,Ash JD,Fox BA,Farris AD,Bzik DJ,Lang ML,Blader IJ

11-0333 was used in Flow cytometry/Cell sorting to investigate the mechanisms by which the retina protects itself from CD4 T-cell-mediated immune damage in retinal immune responses.