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CD45R (B220) Antibody (11-0460-82) in Flow
Wistar rat splenocytes were stained with 0.5 µg of Mouse IgG2b kappa Isotype Control, FITC (Product # 11-4732-82) (blue histogram) or 0.5 µg of CD45R Monoclonal Antibody, FITC (purple histogram). Total viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18).
产品信息
11-0460-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Rat
已发表种属
Mouse,
Rat
宿主/亚型
Mouse
/ IgG2b, kappa
分类
Monoclonal
类型
Antibody
克隆号
HIS24
偶联物
激发/发射光谱
498/517 nm
查看光谱
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_465075
产品详细信息
Description: The HIS24 monoclonal antibody reacts with an isoform of CD45 expressed by rat B cells. The antigen recognized by HIS24 is found on B cells from the early stage of B cell development in the bone marrow to mature peripheral B cells, but not on plasma cells. HIS24 recognizes a 205 kDa protein by immunoblotting. CD45, also known as Leukocyte Common Antigen (LCA), has tyrosine phosphatase enzymatic activity in its cytoplasmic portion and plays an important role in activation of lymphocytes.
Applications Reported: This HIS24 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.
Applications Tested: This HIS24 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This HIS24 antibody has been tested by immunohistochemistry of frozen rat tissue and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
CD45R (PTPRC) is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. CD45 is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists in multiple isoforms as a result of alternative splicing, and these isoforms differ in their extracellular domains, but share identical transmembrane and cytoplasmic domains. Further, these CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. In addition to its role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. Diseases associated with CD45 dysfunction include Severe Combined Immunodeficiency (T-cell negative, B-Cell Negative) and Hepatitis C.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CD45; L-CA; leucocyte common antigen; Leukocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; Protein tyrosine phosphatase, receptor-type, c polypeptide; PTPRC; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein
基因别名: CD45; L-CA; Lca; Ptprc; RT7; T200
UniProt ID: (Rat) P04157
Entrez Gene ID: (Rat) 24699
activation of MAPK activity
negative regulation of T cell mediated cytotoxicity
positive regulation of T cell mediated cytotoxicity
negative regulation of cytokine-mediated signaling pathway
hematopoietic progenitor cell differentiation
immunoglobulin biosynthetic process
positive regulation of T cell mediated immunity
regulation of humoral immune response mediated by circulating immunoglobulin
positive regulation of humoral immune response mediated by circulating immunoglobulin
negative regulation of protein kinase activity
protein dephosphorylation
negative regulation of cell adhesion involved in substrate-bound cell migration
leukocyte cell-cell adhesion
response to gamma radiation
dephosphorylation
B cell differentiation
T cell differentiation
positive regulation of B cell proliferation
negative regulation of protein autophosphorylation
heterotypic cell-cell adhesion
peptidyl-tyrosine dephosphorylation
T cell proliferation
B cell proliferation
positive regulation of T cell proliferation
positive regulation of MAPK cascade
cell cycle phase transition
positive thymic T cell selection
negative thymic T cell selection
regulation of B cell differentiation
positive regulation of T cell differentiation
positive regulation of gamma-delta T cell differentiation
positive regulation of alpha-beta T cell proliferation
positive regulation of isotype switching to IgG isotypes
bone marrow development
stem cell development
negative regulation of peptidyl-tyrosine phosphorylation
T cell receptor signaling pathway
B cell receptor signaling pathway
regulation of B cell receptor signaling pathway
positive regulation of antigen receptor-mediated signaling pathway
release of sequestered calcium ion into cytosol
defense response to virus
regulation of cell cycle
positive regulation of hematopoietic stem cell migration
positive regulation of stem cell proliferation
regulation of extrinsic apoptotic signaling pathway
positive regulation of extrinsic apoptotic signaling pathway
