Invitrogen

CD103 (Integrin alpha E) Monoclonal Antibody (2E7), FITC, eBioscience™

CD103 (Integrin alpha E) Antibody in Flow Cytometry (Flow)

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CD103 (Integrin alpha E) Antibody (11-1031-82) in Flow

Staining of C57Bl/6 lymph node cells with Anti-Mouse CD3e APC (Product # 17-0031-82) and 1.0 µg of Armenian Hamster IgG Isotype Control FITC (Product # 11-4888-81) (left) or 1.0 µg of Anti-Mouse CD103 (Integrin alpha E) FITC (right). Cells in the lymphocyte gate were used for analysis.

CD103 (Integrin alpha E) Antibody in Immunohistochemistry (IHC)

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产品信息

11-1031-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

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免疫组化（冰冻） (IHC (F))

查看2篇已发表文章 2篇已发表文章

免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

1 µg/test

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产品规格

种属反应

Mouse

已发表种属

Human, Mouse

宿主/亚型

Armenian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

2E7

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_465176

产品详细信息

Description: The 2E7 monoclonal antibody reacts with mouse CD103, the alphaE integrin. CD103 non-covalently associates with integrin beta7 and is expressed on all intraepithelial lymphocytes, a small subset of peripheral lymphocytes, and dendritic epidermal T cells (DEC). Epithelial cell antigen E-cadherin binds to CD103 and mediates homing of lymphocytes to the intestinal epithelium.

Applications Reported: The 2E7 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 2E7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD103 (ITGAE, Integrin alpha E) is molecule that is expressed on mucosa-associated T lymphocytes and activated cells and on subet of TGF beta -1 cells. In particular, CD103 includes a 150kDa alpha chain and a 120kDa beta chain. CD103 is expressed by 0.5 to 2% of resting lymphocytes in peripheral blood and lymphoid organs. CD103 antibody stains a few bone marrow cells and, rarely, peripheral blood lymphocytes. CD103 is part of the integrin family which are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. ITGAE encodes an I-domain-containing alpha integrin that undergoes post-translational cleavage in the extracellular domain, yielding disulfide-linked heavy and light chains. In combination with the beta 7 integrin, CD103 protein forms the E-cadherin binding integrin known as the human mucosal lymphocyte-1 antigen. CD103 is preferentially expressed in human intestinal intraepithelial lymphocytes (IEL), and in addition to a role in adhesion, it may serve as an accessory molecule for IEL activation. Diseases associated with CD103 dysfunction include hairy cell leukemia and splenic marginal zone lymphoma.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Profiling of Tregs across tissues reveals plasticity in ST2 expression and hierarchies in tissue-specific phenotypes.

iScience

Spath S,Roan F,Presnell SR,Höllbacher B,Ziegler SF

Published figure using CD103 (Integrin alpha E) monoclonal antibody (Product # 11-1031-82) in Flow Cytometry

Fri Sep 16 00:00:00 UTC 2022

An intranasal lentiviral booster reinforces the waning mRNA vaccine-induced SARS-CoV-2 immunity that it targets to lung mucosa.

Molecular therapy : the journal of the American Society of Gene Therapy

Vesin B,Lopez J,Noirat A,Authié P,Fert I,Le Chevalier F,Moncoq F,Nemirov K,Blanc C,Planchais C,Mouquet H,Guinet F,Hardy D,Vives FL,Gerke C,Anna F,Bourgine M,Majlessi L,Charneau P

11-1031-82 was used in Flow cytometry/Cell sorting to demonstrate the suitability of the LV::SBeta-2P vaccine candidate as an intranasal booster against COVID-19. LV::SBeta-2P vaccination was also fully protective against Omicron infection of the lungs and central nervous system, in the highly susceptible B6.K18-hACE2IP-THV transgenic mice.

Wed Sep 07 00:00:00 UTC 2022

STING Activated Tumor-Intrinsic Type I Interferon Signaling Promotes CXCR3 Dependent Antitumor Immunity in Pancreatic Cancer.

Cellular and molecular gastroenterology and hepatology

Vonderhaar EP,Barnekow NS,McAllister D,McOlash L,Eid MA,Riese MJ,Tarakanova VL,Johnson BD,Dwinell MB

Published figure using CD103 (Integrin alpha E) monoclonal antibody (Product # 11-1031-82) in Flow Cytometry

Mon Jan 24 00:00:00 UTC 2022

Macrophages Maintain Epithelium Integrity by Limiting Fungal Product Absorption.

Cell

Chikina AS,Nadalin F,Maurin M,San-Roman M,Thomas-Bonafos T,Li XV,Lameiras S,Baulande S,Henri S,Malissen B,Lacerda Mariano L,Barbazan J,Blander JM,Iliev ID,Matic Vignjevic D,Lennon-Duménil AM

Published figure using CD103 (Integrin alpha E) monoclonal antibody (Product # 11-1031-82) in Flow Cytometry

Thu Oct 15 00:00:00 UTC 2020

Contact-dependent delivery of IL-2 by dendritic cells to CD4 T cells in the contraction phase promotes their long-term survival.

Protein & cell

Tong D,Zhang L,Ning F,Xu Y,Hu X,Shi Y

Published figure using CD103 (Integrin alpha E) monoclonal antibody (Product # 11-1031-82) in Flow Cytometry

Sat Feb 01 00:00:00 UTC 2020

Inhibition of the Wnt/β-catenin pathway enhances antitumor immunity in ovarian cancer.

Therapeutic advances in medical oncology

Doo DW,Meza-Perez S,Londoño AI,Goldsberry WN,Katre AA,Boone JD,Moore DJ,Hudson CT,Betella I,McCaw TR,Gangrade A,Bao R,Luke JJ,Yang ES,Birrer MJ,Starenki D,Cooper SJ,Buchsbaum DJ,Norian LA,Randall TD,Arend RC

Published figure using CD103 (Integrin alpha E) monoclonal antibody (Product # 11-1031-82) in Flow Cytometry

Thu Apr 14 00:00:00 UTC 2022

Human and Mouse Transcriptome Profiling Identifies Cross-Species Homology in Pulmonary and Lymph Node Mononuclear Phagocytes.

Cell reports

Leach SM,Gibbings SL,Tewari AD,Atif SM,Vestal B,Danhorn T,Janssen WJ,Wager TD,Jakubzick CV

Published figure using CD103 (Integrin alpha E) monoclonal antibody (Product # 11-1031-82) in Flow Cytometry

Tue Nov 03 00:00:00 UTC 2020

Acid ceramidase of macrophages traps herpes simplex virus in multivesicular bodies and protects from severe disease.

Nature communications

Lang J,Bohn P,Bhat H,Jastrow H,Walkenfort B,Cansiz F,Fink J,Bauer M,Olszewski D,Ramos-Nascimento A,Duhan V,Friedrich SK,Becker KA,Krawczyk A,Edwards MJ,Burchert A,Huber M,Friebus-Kardash J,Göthert JR,Hardt C,Probst HC,Schumacher F,Köhrer K,Kleuser B,Babiychuk EB,Sodeik B,Seibel J,Greber UF,Lang PA,Gulbins E,Lang KS

11-1031-82 was used in Flow Cytometry to show that macrophages take up HSV-1 via endocytosis and transport the virions into multivesicular bodies (MVBs).

Thu Mar 12 00:00:00 UTC 2020

Programming Multifaceted Pulmonary T Cell Immunity by Combination Adjuvants.

Cell reports. Medicine

Marinaik CB,Kingstad-Bakke B,Lee W,Hatta M,Sonsalla M,Larsen A,Neldner B,Gasper DJ,Kedl RM,Kawaoka Y,Suresh M

11-1031 was used in Flow cytometry/Cell sorting to provide further insights into vaccine-induced multifaceted mucosal T cell immunity with implications in the development of vaccines against respiratorypathogens, including influenza virus and SARS-CoV-2.

Tue Sep 22 00:00:00 UTC 2020

Bilateral Effect of the Unilateral Corneal Nerve Cut on Both Ocular Surface and Lacrimal Gland.

Investigative ophthalmology & visual science

Lee HK,Kim KW,Ryu JS,Jeong HJ,Lee SM,Kim MK

11-1031 was used in Flow cytometry/Cell sorting to demonstrate that a unilateral cut of the corneal nerve results in activation of immune cells on the ocular surface and dysregulation of lacrimal secretion bilaterally through the bidirectional neuronal signals.

Wed Jan 02 00:00:00 UTC 2019

Virus-specific memory T cells populate tumors and can be repurposed for tumor immunotherapy.

Nature communications

Rosato PC,Wijeyesinghe S,Stolley JM,Nelson CE,Davis RL,Manlove LS,Pennell CA,Blazar BR,Chen CC,Geller MA,Vezys V,Masopust D

Published figure using CD103 (Integrin alpha E) monoclonal antibody (Product # 11-1031-82) in Flow Cytometry

Mon Feb 04 00:00:00 UTC 2019

CSF1R antagonism limits local restimulation of antiviral CD8⁺ T cells during viral encephalitis.

Journal of neuroinflammation

Funk KE,Klein RS

Published figure using CD103 (Integrin alpha E) monoclonal antibody (Product # 11-1031-82) in Flow Cytometry

Thu Jan 31 00:00:00 UTC 2019

MicroRNA-150 controls differentiation of intraepithelial lymphocytes through TGF-β receptor II regulation.

The Journal of allergy and clinical immunology

Seo SH,Jang MS,Kim DJ,Kim SM,Oh SC,Jung CR,Park Y,Ha SJ,Jung H,Park YJ,Yoon SR,Choi I,Kim TD

11-1031-82 was used in Flow Cytometry to investigate the regulation of intraepithelial lymphocyte differentiation and the underlying mechanisms in mice.

Sun Apr 01 00:00:00 UTC 2018

Reactive glia promote development of CD103⁺ CD69⁺ CD8⁺ T-cells through programmed cell death-ligand 1 (PD-L1).

Immunity, inflammation and disease

Prasad S,Hu S,Sheng WS,Chauhan P,Lokensgard JR

11-1031 was used in Flow cytometry/Cell sorting to demonstrate a role for activated glia in promoting development of bTRM through the PD-1: PD-L1 pathway.

Fri Jun 01 00:00:00 UTC 2018

Mrp1 is involved in lipid presentation and iNKT cell activation by Streptococcus pneumoniae.

Nature communications

Chandra S,Gray J,Kiosses WB,Khurana A,Hitomi K,Crosby CM,Chawla A,Fu Z,Zhao M,Veerapen N,Richardson SK,Porcelli SA,Besra G,Howell AR,Sharma S,Peters B,Kronenberg M

11-1031 was used in Immunocytochemistry-immunoflourescence to elucidate the CD1d presentation pathway.

Mon Oct 15 00:00:00 UTC 2018

NLRP12 attenuates colon inflammation by maintaining colonic microbial diversity and promoting protective commensal bacterial growth.

Nature immunology

Chen L,Wilson JE,Koenigsknecht MJ,Chou WC,Montgomery SA,Truax AD,Brickey WJ,Packey CD,Maharshak N,Matsushima GK,Plevy SE,Young VB,Sartor RB,Ting JP

Published figure using CD103 (Integrin alpha E) monoclonal antibody (Product # 11-1031-82) in Flow Cytometry

Mon May 01 00:00:00 UTC 2017

Deletion of PIKfyve alters alveolar macrophage populations and exacerbates allergic inflammation in mice.

The EMBO journal

Kawasaki T,Ito K,Miyata H,Akira S,Kawai T

Published figure using CD103 (Integrin alpha E) monoclonal antibody (Product # 11-1031-82) in Flow Cytometry

Wed Jun 14 00:00:00 UTC 2017

Mesenteric lymph node CD11b^- CD103⁺ PD-L1^High dendritic cells highly induce regulatory T cells.

Immunology

Shiokawa A,Kotaki R,Takano T,Nakajima-Adachi H,Hachimura S

11-1031 was used in Flow cytometry/Cell sorting to investigate the expression of these molecules on MLN CD11c+ cells.

Fri Sep 01 00:00:00 UTC 2017

Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells.

Journal of visualized experiments : JoVE

Lyons-Cohen MR,Thomas SY,Cook DN,Nakano H

Published figure using CD103 (Integrin alpha E) monoclonal antibody (Product # 11-1031-82) in Immunofluorescence

Wed Apr 05 00:00:00 UTC 2017

Host-Protozoan Interactions Protect from Mucosal Infections through Activation of the Inflammasome.

Cell

Chudnovskiy A,Mortha A,Kana V,Kennard A,Ramirez JD,Rahman A,Remark R,Mogno I,Ng R,Gnjatic S,Amir ED,Solovyov A,Greenbaum B,Clemente J,Faith J,Belkaid Y,Grigg ME,Merad M

Published figure using CD103 (Integrin alpha E) monoclonal antibody (Product # 11-1031-82) in Flow Cytometry

Thu Oct 06 00:00:00 UTC 2016

Brain-resident memory T cells represent an autonomous cytotoxic barrier to viral infection.

The Journal of experimental medicine

Steinbach K,Vincenti I,Kreutzfeldt M,Page N,Muschaweckh A,Wagner I,Drexler I,Pinschewer D,Korn T,Merkler D

11-1031 was used in Immunofluorescence on frozen tissues to characterise the functioning of brain-resident memory T cells in an animal model of viral infection.

Mon Jul 25 00:00:00 UTC 2016

Resident T Cells Are Unable To Control Herpes Simplex Virus-1 Activity in the Brain Ependymal Region during Latency.

Journal of immunology (Baltimore, Md. : 1950)

Menendez CM,Jinkins JK,Carr DJ

Published figure using CD103 (Integrin alpha E) monoclonal antibody (Product # 11-1031-82) in Flow Cytometry

Mon Aug 15 00:00:00 UTC 2016

Diet-dependent, microbiota-independent regulation of IL-10-producing lamina propria macrophages in the small intestine.

Scientific reports

Ochi T,Feng Y,Kitamoto S,Nagao-Kitamoto H,Kuffa P,Atarashi K,Honda K,Teitelbaum DH,Kamada N

Published figure using CD103 (Integrin alpha E) monoclonal antibody (Product # 11-1031-82) in Flow Cytometry

Wed Jun 15 00:00:00 UTC 2016

A reproducible method for the expansion of mouse CD8+ T lymphocytes.

Journal of immunological methods

Lewis MD,de Leenheer E,Fishman S,Siew LK,Gross G,Wong FS

11-1031 was used in Flow cytometry/Cell sorting to report a tissue culture protocol for the reliable expansion of CD8+ T-cells derived from murine spleens.

Sun Feb 01 00:00:00 UTC 2015

Immune complexes stimulate CCR7-dependent dendritic cell migration to lymph nodes.

Nature medicine

Clatworthy MR,Aronin CE,Mathews RJ,Morgan NY,Smith KG,Germain RN

Published figure using CD103 (Integrin alpha E) monoclonal antibody (Product # 11-1031-82) in Flow Cytometry

Mon Dec 01 00:00:00 UTC 2014

Lung dendritic cells undergo maturation and polarization towards a T helper type 2-stimulating phenotype in a mouse model of asthma: Role of nerve growth factor.

Experimental and therapeutic medicine

Qin Q,Wang Z,Pan P,Cao Z,Xia Q,Tan H,Hu C

Sat Nov 01 00:00:00 UTC 2014

Systemic sensitization with the protein allergen ovalbumin augments local sensitization in atopic dermatitis.

Journal of inflammation research

Yoo J,Manicone AM,McGuire JK,Wang Y,Parks WC

11-1031 was used in Flow cytometry/Cell sorting to reveal a mouse model of atopic dermatitis that reveals novel insights into the pathogenesis of this complex disease.

Thu Mar 27 00:00:00 UTC 2014

MHC class I-restricted myelin epitopes are cross-presented by Tip-DCs that promote determinant spreading to CD8⁺ T cells.

Nature immunology

Ji Q,Castelli L,Goverman JM

11-1031 was used in Flow cytometry/Cell sorting to investigate the mechanisms underlying the role of CD8(+) T cells in the pathogenesis of multiple sclerosis.

Fri Mar 01 00:00:00 UTC 2013

Protection of islet grafts through transforming growth factor-β-induced tolerogenic dendritic cells.

Diabetes

Thomas DC,Wong FS,Zaccone P,Green EA,Wållberg M

11-1031 was used in Flow cytometry/Cell sorting to investigate methods to achieve long-term β-islet graft tolerance for diabetes type 1, showing that islets can be protected through expression of TGF-β.

Sun Sep 01 00:00:00 UTC 2013

PD-L2 modulates asthma severity by directly decreasing dendritic cell IL-12 production.

Mucosal immunology

Lewkowich IP,Lajoie S,Stoffers SL,Suzuki Y,Richgels PK,Dienger K,Sproles AA,Yagita H,Hamid Q,Wills-Karp M

11-1031 was used in Flow cytometry/Cell sorting to investigate the mechanism by which PD-L2 modulates asthma severity in human and mouse lung samples.

Mon Jul 01 00:00:00 UTC 2013

Changes in lamina propria dendritic cells on the oral administration of exogenous protein antigens during weaning.

Cytotechnology

Ohue R,Nakamoto M,Kitabatake N,Tani F

11-1031 was used in Flow cytometry/Cell sorting to suggest that the behavior of tolerogenic PDCA-1(+) DCs may change at weaning with the removal of the immunoprotective components of maternal milk.

Tue May 01 00:00:00 UTC 2012

H2 control of natural T regulatory cell frequency in the lymph node correlates with susceptibility to day 3 thymectomy-induced autoimmune disease.

Journal of immunology (Baltimore, Md. : 1950)

del Rio R,Sun Y,Alard P,Tung KS,Teuscher C

11-1031 was used in Flow cytometry/Cell sorting to confirm that quantitative trait loci on a range of chromosomes control susceptibility to autoimmune ovarian dysgenesis.

Sat Jan 01 00:00:00 UTC 2011

Down-regulation of Gfi-1 expression by TGF-beta is important for differentiation of Th17 and CD103+ inducible regulatory T cells.

The Journal of experimental medicine

Zhu J,Davidson TS,Wei G,Jankovic D,Cui K,Schones DE,Guo L,Zhao K,Shevach EM,Paul WE

11-1031 was used in Flow cytometry/Cell sorting to show that Gfi-1, a transcriptional repressor, has a critical role in enhancing Th2 cell expansion and repressing induction of Th17 and CD103(+) iTreg cells.

Mon Feb 16 00:00:00 UTC 2009

Trafficking of antigen-specific CD8+ T lymphocytes to mucosal surfaces following intramuscular vaccination.

Journal of immunology (Baltimore, Md. : 1950)

Kaufman DR,Liu J,Carville A,Mansfield KG,Havenga MJ,Goudsmit J,Barouch DH

11-1031 was used in Flow cytometry/Cell sorting to evaluate mucosal cellular immune responses in mice and rhesus monkeys after systemic immunisation with various replication-incompetent recombinant adenovirus vectors.