Invitrogen

Ly-49H Monoclonal Antibody (3D10), FITC, eBioscience™

Ly-49H Antibody in Flow Cytometry (Flow)

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Ly-49H Antibody (11-5886-82) in Flow

Staining of C57BL/6 splenocytes with Anti-Mouse CD49b (Integrin alpha 2) APC (Product # 17-5971-82) and 0.25 µg of Mouse IgG1 kappa Isotype Control FITC (Product # 11-4714-42) (left) or 0.25 µg of Anti-Mouse Ly-49H FITC (right). Total viable cells were used for analysis.

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产品信息

11-5886-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.5 µg/test

查看10篇已发表文章 10篇已发表文章

中和实验 (Neu)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

3D10

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_1257159

产品详细信息

Description: The monoclonal antibody 3D10 recognizes Ly-49H, a member of the C-type lectin Ly-49 multigene family of receptors found on natural killer cells. Ly-49H, like Ly-49D, lacks the characteristic ITIM (immunoreceptor tyrosine-based inhibitory motif) and contains an arginine in the transmembrane domain implying these molecules act as activation receptors. Ly-49H is expressed in a subset of NK cells but not NKT (NK1.1CD3+) cells. Natural killer cells can express several Ly-49 proteins. It has been shown that there is preferential staining of Ly-49H with Ly-49D (Product # 00-5523-00) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Ly49H is a member of the C-type lectin Ly-49 multigene family of receptors found on natural killer cells. Ly49H, like Ly49D, lacks the characteristic ITAM (immunoreceptor tyrosine-based inhibitory motif) and contains an arginine in the transmembrane domain implying these molecules act as activation receptors. Ly49H is expressed in a subset of NK cells but not NKT (NK1.1/CD3+) cells. Natural killer cells can express several Ly49 proteins.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Perinatal murine cytomegalovirus infection reshapes the transcriptional profile and functionality of NK cells.

Nature communications

Rožmanić C,Lisnić B,Pribanić Matešić M,Mihalić A,Hiršl L,Park E,Lesac Brizić A,Indenbirken D,Viduka I,Šantić M,Adler B,Yokoyama WM,Krmpotić A,Juranić Lisnić V,Jonjić S,Brizić I

Published figure using Ly-49H monoclonal antibody (Product # 11-5886-82) in Flow Cytometry

Thu Oct 12 00:00:00 UTC 2023

Tissue-specific transcriptional profiles and heterogeneity of natural killer cells and group 1 innate lymphoid cells.

Cell reports. Medicine

Lopes N,Galluso J,Escalière B,Carpentier S,Kerdiles YM,Vivier E

11-5886-82 was used in Flow cytometry/Cell sorting to reveal that the expressions of EOMES, GZMA, IRF8, JAK1, NKG7, PLEK, PRF1, and ZEB2 define NK cells and that IL7R, LTB, and RGS1 differentiate ILC1s from NK cells in mice and humans.

Tue Nov 15 00:00:00 UTC 2022

Multi-tissue single-cell analysis deconstructs the complex programs of mouse natural killer and type 1 innate lymphoid cells in tissues and circulation.

Immunity

McFarland AP,Yalin A,Wang SY,Cortez VS,Landsberger T,Sudan R,Peng V,Miller HL,Ricci B,David E,Faccio R,Amit I,Colonna M

11-5886-82 was used in Flow cytometry/Cell sorting to identify gene expression programs of tissue-specific ILC1s, tissue-specific NK cells, and non-tissue-specific populations in blood, spleen, and other tissues largely corresponding to circulating cells.

Tue Jun 08 00:00:00 UTC 2021

Coordinated Viral Control by Cytotoxic Lymphocytes Ensures Optimal Adaptive NK Cell Responses.

Cell reports

Diaz-Salazar C,Sun JC

11-5886 was used in Flow cytometry/Cell sorting to support a mechanism whereby cytotoxic innate and adaptive lymphocytes cooperate to ensure viral clearance and the establishment of robust clonal NK cell responses.

Tue Sep 22 00:00:00 UTC 2020

Divergent Role for STAT5 in the Adaptive Responses of Natural Killer Cells.

Cell reports

Wiedemann GM,Grassmann S,Lau CM,Rapp M,Villarino AV,Friedrich C,Gasteiger G,O'Shea JJ,Sun JC

11-5886 was used in Flow cytometry/Cell sorting to investigate how STAT5 regulates transcription during viral infection.

Tue Dec 15 00:00:00 UTC 2020

Cytomegalovirus Infection Drives Avidity Selection of Natural Killer Cells.

Immunity

Adams NM,Geary CD,Santosa EK,Lumaquin D,Le Luduec JB,Sottile R,van der Ploeg K,Hsu J,Whitlock BM,Jackson BT,Weizman OE,Huse M,Hsu KC,Sun JC

11-5886 was used in Flow cytometry/Cell sorting to delineate how heterogeneity in NK cell avidity diversifies NK cell effector function during antiviral immunity, and how avidity selection might serve to produce the most potent memory NK cells.

Tue Jun 18 00:00:00 UTC 2019

Epigenetic control of innate and adaptive immune memory.

Nature immunology

Lau CM,Adams NM,Geary CD,Weizman OE,Rapp M,Pritykin Y,Leslie CS,Sun JC

11-5886 was used in Flow cytometry/Cell sorting to demonstrate that differentiating and 'memory' natural killer cells possess distinct chromatin accessibility states, and that their epigenetic profiles reveal a 'poised' regulatory programme at the memory stage.

Sat Sep 01 00:00:00 UTC 2018

Non-redundant ISGF3 Components Promote NK Cell Survival in an Auto-regulatory Manner during Viral Infection.

Cell reports

Geary CD,Krishna C,Lau CM,Adams NM,Gearty SV,Pritykin Y,Thomsen AR,Leslie CS,Sun JC

11-5886 was used in Flow cytometry/Cell sorting to study the role of individual ISGF3 components as regulators of the natural killer cell response to viral infection.

Tue Aug 21 00:00:00 UTC 2018

Natural killer cells attenuate cytomegalovirus-induced hearing loss in mice.

PLoS pathogens

Almishaal AA,Mathur PD,Hillas E,Chen L,Zhang A,Yang J,Wang Y,Yokoyama WM,Firpo MA,Park AH

Published figure using Ly-49H monoclonal antibody (Product # 11-5886-82) in Neutralization

Tue Aug 01 00:00:00 UTC 2017

Trypanosoma brucei Co-opts NK Cells to Kill Splenic B2 B Cells.

PLoS pathogens

Frenkel D,Zhang F,Guirnalda P,Haynes C,Bockstal V,Radwanska M,Magez S,Black SJ

11-5886 was used in Flow cytometry/Cell sorting to investigate the mechanism by which natural killer cells in Trypanosoma brucei infected mice kill B cells, suppress humoral immunity and induce early mortality.

Fri Jul 01 00:00:00 UTC 2016

Diacylglycerol Kinase ζ Is a Target To Enhance NK Cell Function.

Journal of immunology (Baltimore, Md. : 1950)

Yang E,Singh BK,Paustian AM,Kambayashi T

11-5886 was used in Flow cytometry/Cell sorting to investigate methods to enhance NK cell function, showing that diacylglycerol kinase ζ is a target for enhancement of NK function.

Mon Aug 01 00:00:00 UTC 2016

生物信息学

蛋白别名: cytomegalovirus resistance 1; Killer cell lectin-like receptor 8; Ly-49h; Lymphocyte antigen 49h; T-cell surface glycoprotein Ly-49H

查看更多收起

基因别名: Cmv-1; Cmv1; Klra8; Ly-49h; Ly49-h; Ly49H; Ly49u<129>

查看更多收起

UniProt ID: (Mouse) Q60682

查看更多收起

Entrez Gene ID: (Mouse) 16639

查看更多收起

功能

carbohydrate binding

参与通路

cell adhesion response to virus

Ly-49H Monoclonal Antibody (3D10), FITC, eBioscience™

Ly-49H Antibody (11-5886-82) in Flow

产品信息

11-5886-82

流式细胞分析 (Flow)

中和实验 (Neu)

种属反应

已发表种属

宿主/亚型

推荐的同型对照

分类

类型

克隆号

偶联物

激发/发射光谱

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

How to use the Panel Builder

生物信息学

性能保障

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