Invitrogen

CD24 Monoclonal Antibody (30-F1), PE, eBioscience™

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CD24 Antibody (12-0241-82) in Flow

Stem cells (Dayton, Ohio) 2021 - 5 FIGURE Adipose stem cells (ASCs) are less numerous in ingWAT and BAT from in Lyl1 -/- mice, and Lyl1 is not expressed in the adipocyte lineage. A, Dot plots showing FACS analysis of SVFs isolated from ingWAT of a 6-week-old WT mouse and the gating strategy to isolate preadipocytes (CD140a+ CD24-, green) and ASCs (CD140a+ CD24+, blue). B, ASCs and preadipocytes were quantified by flow cytometry in SVFs iso... View More

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产品信息

12-0241-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.125 µg/test

查看16篇已发表文章 16篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Human, Mouse

宿主/亚型

Rat / IgG2c, kappa

分类

Monoclonal

类型

Antibody

克隆号

30-F1

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_465599

产品详细信息

Description: The 30-F1 monoclonal antibody reacts with the mouse CD24 molecule, also known as Heat Stable Antigen (HSA). This 35-50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes and myeloid lineage. The expression of CD24 detected by 30-F1 has been used to resolve stages of B lymphopoiesis in mouse bone marrow. It has been reported that P-selectin (CD62P) binds to CD24. CD24 is a variably glycosylated molecule resulting in heterogeneity of molecular mass of this antigen on cells of different lineages and different antibodies to CD24 exhibit subtle differences in staining level on lymphocyte populations.

Applications Reported: The 30-F1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 30-F1 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD24 is a two-chain glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed at multiple stages of B-cell development, beginning with the bone marrow pro-B-cell compartment and continuing through mature, surface Ig positive B-cells. Plasma cell expression is very low or negative. CD24 is also expressed on the majority of B-lineage acute lymphoblastic leukemias, B-cell CCLs and B-cell non-Hodgkin's lymphomas. CD24 may play a role in regulation of B-cell proliferation and maturation, and control of autoimmunity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

In Lyl1^-/- mice, adipose stem cell vascular niche impairment leads to premature development of fat tissues.

Stem cells (Dayton, Ohio)

Hussain A,Deleuze V,El Kebriti L,Turali H,Pirot N,Glasson Y,Mathieu D,Pinet V

Published figure using CD24 monoclonal antibody (Product # 12-0241-82) in Flow Cytometry

Fri Jan 01 00:00:00 UTC 2021

Cosmc is required for T cell persistence in the periphery.

Glycobiology

Cutler CE,Jones MB,Cutler AA,Mener A,Arthur CM,Stowell SR,Cummings RD

12-0241-82 was used in Flow Cytometry to demonstrate that extended O-glycosylation is required for the establishment and maintenance of the peripheral T cell population.

Mon Oct 21 00:00:00 UTC 2019

Cbl Ubiquitin Ligases Control B Cell Exit from the Germinal-Center Reaction.

Immunity

Li X,Gadzinsky A,Gong L,Tong H,Calderon V,Li Y,Kitamura D,Klein U,Langdon WY,Hou F,Zou YR,Gu H

12-0241-82 was used in Flow Cytometry to show that deleting E3 ubiquitin ligases Cbl and Cbl-b (Cbls) in GC B cells results in the early exit of high-affinity antigen-specific B cells from the GC reaction and thus impaired clonal expansion.

Tue Mar 20 00:00:00 UTC 2018

Gli3 in fetal thymic epithelial cells promotes thymocyte positive selection and differentiation by repression of Shh.

Development (Cambridge, England)

Solanki A,Yanez DC,Ross S,Lau CI,Papaioannou E,Li J,Saldaña JI,Crompton T

Wed Jan 31 00:00:00 UTC 2018

Adipocyte Accumulation in the Bone Marrow during Obesity and Aging Impairs Stem Cell-Based Hematopoietic and Bone Regeneration.

Cell stem cell

Ambrosi TH,Scialdone A,Graja A,Gohlke S,Jank AM,Bocian C,Woelk L,Fan H,Logan DW,Schürmann A,Saraiva LR,Schulz TJ

12-0241 was used in Flow cytometry/Cell sorting to specify the cellular identities of the adipogenic and osteogenic lineages of the bone.

Thu Jun 01 00:00:00 UTC 2017

Breast cancer stem cells expressing different stem cell markers exhibit distinct biological characteristics.

Molecular medicine reports

Shao J,Fan W,Ma B,Wu Y

12-0241 was used in Flow cytometry/Cell sorting to demonstrate that breast cancer stem cells are heterogeneous, and they exhibit distinct biological characteristics.

Thu Dec 01 00:00:00 UTC 2016

PRDM14 promotes RAG-dependent Notch1 driver mutations in mouse T-ALL.

Biology open

Carofino BL,Ayanga B,Tracey LJ,Brooke-Bisschop T,Justice MJ

12-0241 was used in Flow cytometry/Cell sorting to investigate how PRDM14 promotes RAG-dependent Notch1 driver mutations in mouse T-ALL.

Sun May 15 00:00:00 UTC 2016

Neuregulin autocrine signaling promotes self-renewal of breast tumor-initiating cells by triggering HER2/HER3 activation.

Cancer research

Lee CY,Lin Y,Bratman SV,Feng W,Kuo AH,Scheeren FA,Engreitz JM,Varma S,West RB,Diehn M

12-0241 was used in Flow cytometry/Cell sorting to demonstrate a mechanism through which HER2-targeting therapies could benefit a larger number of breast cancer patients.

Wed Jan 01 00:00:00 UTC 2014

GATA3 suppresses metastasis and modulates the tumour microenvironment by regulating microRNA-29b expression.

Nature cell biology

Chou J,Lin JH,Brenot A,Kim JW,Provot S,Werb Z

12-0241 was used in Flow cytometry/Cell sorting to study how the GATA3-miR-29b axis regulates the tumour microenvironment, and inhibits metastasis to open up possibilities for therapeutic intervention in breast cancer.

Fri Feb 01 00:00:00 UTC 2013

Notch2 genetic fate mapping reveals two previously unrecognized mammary epithelial lineages.

Nature cell biology

Šale S,Lafkas D,Artavanis-Tsakonas S

Published figure using CD24 monoclonal antibody (Product # 12-0241-82) in Flow Cytometry

Wed May 01 00:00:00 UTC 2013

Leptin receptor maintains cancer stem-like properties in triple negative breast cancer cells.

Endocrine-related cancer

Zheng Q,Banaszak L,Fracci S,Basali D,Dunlap SM,Hursting SD,Rich JN,Hjlemeland AB,Vasanji A,Berger NA,Lathia JD,Reizes O

12-0241 was used in Flow cytometry/Cell sorting to explore the role of the leptin receptor in the maintenance of cancer stem cells.

Sun Dec 01 00:00:00 UTC 2013

A mouse model for inducible overexpression of Prdm14 results in rapid-onset and highly penetrant T-cell acute lymphoblastic leukemia (T-ALL).

Disease models & mechanisms

Carofino BL,Ayanga B,Justice MJ

12-0241 was used in Flow cytometry/Cell sorting to demonstrate that increased expression of Prdm14 drives development of T-Cell acute lymphoblastic leukemia in mice.

Fri Nov 01 00:00:00 UTC 2013

Nuclear export of histone deacetylase 7 during thymic selection is required for immune self-tolerance.

The EMBO journal

Kasler HG,Lim HW,Mottet D,Collins AM,Lee IS,Verdin E

12-0241 was used in Flow cytometry/Cell sorting to examine the effect of blocking TCR-dependent nuclear export of HDAC7 during thymic selection, showing that HDAC7 is required for immune self-tolerance.

Wed Nov 28 00:00:00 UTC 2012

VCAM-1 blockade delays disease onset, reduces disease severity and inflammatory cells in an atopic dermatitis model.

Immunology and cell biology

Chen L,Lin SX,Amin S,Overbergh L,Maggiolino G,Chan LS

12-0241 was used in Flow cytometry/Cell sorting to investigate the functions of ICAM-1 and VCAM-1 in a keratin-14 IL-4-Tg mouse model of atopic dermatitis.

Fri Jun 11 00:00:00 UTC 2010

Immune-induced epithelial to mesenchymal transition in vivo generates breast cancer stem cells.

Cancer research

Santisteban M,Reiman JM,Asiedu MK,Behrens MD,Nassar A,Kalli KR,Haluska P,Ingle JN,Hartmann LC,Manjili MH,Radisky DC,Ferrone S,Knutson KL

12-0241 was used in Flow cytometry/Cell sorting to show that epithelial to mesenchymal transition can produce the breast cancer stem cell phenotype.

Wed Apr 01 00:00:00 UTC 2009

TOX provides a link between calcineurin activation and CD8 lineage commitment.

The Journal of experimental medicine

Aliahmad P,O'Flaherty E,Han P,Goularte OD,Wilkinson B,Satake M,Molkentin JD,Kaye J

12-0241 was used in Flow cytometry/Cell sorting to demonstrate that up-regulation of TOX in double positive thymocytes is calcineurin dependent, linking the signalling pathway to nuclear changes that occur during positive thymocyte selection..

Mon Apr 19 00:00:00 UTC 2004

生物信息学

蛋白别名: CD24; cluster of differentiation 24; FLJ22950; FLJ43543; heat stable antigen; HSA; Ly-52; Lymphocyte antigen 52; M1/69-J11D heat stable antigen; MGC75043; Nectadrin; R13-Ag; Signal transducer CD24; X62 heat stable antigen

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基因别名: Cd24; Cd24a; HSA; Ly-52; nectadrin

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UniProt ID: (Mouse) P24807

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Entrez Gene ID: (Mouse) 12484

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功能

protein binding protein kinase binding carbohydrate binding protein tyrosine kinase activator activity

参与通路