Invitrogen

CD199 (CCR9) Monoclonal Antibody (eBioCW-1.2 (CW-1.2)), PE, eBioscience™

CD199 (CCR9) Antibody in Flow Cytometry (Flow)

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CD199 (CCR9) Antibody (12-1991-82) in Flow

Staining of BALB/c thymocytes with Anti-Mouse CD4 eFluor® 450 (Product # 48-0042-82), Anti-Mouse CD8a APC (Product # 17-0081-82) and 0.25 µg of Mouse IgG2a K Isotype Control PE (Product # 12-4724-81) (left) or 0.25 µg of Anti-Mouse CD199 (CCR9) PE (right). Samples were gated on CD4 single positive (orange histogram) or CD4+CD8+ double positive cells (purple histogram).

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产品信息

12-1991-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.5 µg/test

查看11篇已发表文章 11篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

eBioCW-1.2 (CW-1.2)

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_1257161

产品详细信息

Description: The eBioCW-1.2 monoclonal antibody reacts with mouse CCR9 (CD199), which is the receptor for thymus-expressed chemokine (TECK). CCR9 is a member of the G protein coupled receptor (GPCR) supergene family, and is involved in trafficking of T cell progenitors within the thymus. CCR9 expression during thymocyte development commences at the double-negative (DN) 3 stage (CD4-CD8-CD25+CD44-), peaks in the double-positive (DP) stage (CD4+CD8+CD25-CD44-), and is down-regulated in commited CD4+ or CD8+ single-positive (SP) thymoyctes. CCR9-deficient mice show a mild impairment in thymoycte development. In the periphery, CCR9 is thought to be expressed on naive CD8+ T cells, but not on naive CD4+ T cells.

Applications Reported: This eBioCW-1.2 (CW-1.2) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioCW-1.2 (CW-1.2) antibody has been tested by flow cytometric analysis of mouse thymocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The protein encoded by this gene is a member of the beta chemokine receptor family. It is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. Chemokines and their receptors are key regulators of the thymocytes migration and maturation in normal and inflammation conditions. The specific ligand of this receptor is CCL25. It has been found that this gene is differentially expressed by T lymphocytes of small intestine and colon, suggested a role in the thymocytes recruitment and development that may permit functional specialization of immune responses in different segment of the gastrointestinal tract. This gene is mapped to the chemokine receptor gene cluster region. Two alternatively spliced transcript variants have been described.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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