Invitrogen

CD339 (Jagged 1) Monoclonal Antibody (HMJ1-29), PE, eBioscience™

CD339 (Jagged 1) Antibody in Flow Cytometry (Flow)

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CD339 (Jagged 1) Antibody (12-3391-82) in Flow

Staining of CD8+CD11c+ C57BL/6 splenocytes with 0.5 µg of Armenian Hamster IgG Isotype Control PE (Product # 12-4888-81) (blue histogram) or 0.5 µg of Anti-Mouse CD339 (Jagged 1) PE (purple histogram). Total viable cells were used for analysis.

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产品信息

12-3391-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

1 µg/test

查看4篇已发表文章 4篇已发表文章

产品规格

种属反应

Mouse, Rat

已发表种属

Mouse

宿主/亚型

Armenian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

HMJ1-29

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_1963616

产品详细信息

Description: This HMJ1-29 monoclonal antibody reacts with mouse Jagged-1, one of five type I transmembrane proteins that serves as a Notch receptor ligand. Upon binding the Notch receptor (e.g. Notch 1-4), Jagged-1 undergoes proteolytic cleavage, first by ADAM-family metalloproteases and then by gamma-secretase. The second cleavage event releases an intracellular fragment whose biological function remains controversial. Jagged-1 is expressed by thymic lymphoid and stromal cells, as well as macrophages and CD8+CD11c+ dendritic cells in the spleen.

The HMJ1-29 antibody is reported to crossreact to rat.

Applications Reported: This HMJ1-29 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HMJ1-29 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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12-3391 was used in Flow cytometry/Cell sorting to determine whether specific conventional dendritic cell subsets are involved in allergic airway sensitisation.