Invitrogen

NOTCH3 Monoclonal Antibody (HMN3-133), PE, eBioscience™

NOTCH3 Antibody in Flow Cytometry (Flow)

图: 1 / 1

NOTCH3 Antibody (12-5763-80) in Flow

Staining of CD4+CD8+ (blue histogram) and CD4-CD8-CD25+CD44- (purple histogram) C57BL/6 thymocytes with 0.5 µg of Armenian Hamster IgG Isotype Control PE (Product # 12-4888-81) (left) or 0.25 µg of Anti-Mouse Notch3 PE (right). Total viable cells were used for analysis.

Product Image Gallery

产品信息

12-5763-80

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.5 µg/test

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Armenian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

HMN3-133

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_1548667

产品详细信息

Description: This HMN3-133 monoclonal antibody reacts with mouse Notch3, one of four members of the Notch family of receptors. Notch receptors are 300-kDa single-pass transmembrane proteins. While the extracellular domain contains numerous epidermal growth factor-like repeats for ligand binding, the intracellular domain is involved in cell signaling. Upon binding its membrane-bound ligand (either Delta or Jagged), the Notch receptor undergoes proteolytic cleavage, first by ADAM-family metalloproteases and then by gamma-secretase. The second cleavage event releases the Notch intracellular domain (NICD), which subsequently translocates into the nucleus, heterodimerizes with the DNA-binding protein RBP-J, recruits co-activator molecules, and ultimately activates transcription.

Notch 3 expression has been demonstrated on regulatory T cells and some thymocyte subsets, including CD4-CD8- and CD8SP cells. In addition, this Notch receptor is prevalent in vascular smooth muscle and the central nervous system. In addition to its role in stem cell hematopoiesis, Notch 3 plays a pivotal role in mammalian T cell lineage commitment and thymocyte development. Specifically, Notch 3 has been implicated in the CD4-CD8- double negative (DN) to CD4+CD8+ double positive transition with greatest expression in DN3 (CD4-CD8-CD25+CD44-) cells. Notch 3 may also be involved in regulating the pre-TCR checkpoint via crosstalk with the NFkappaB and E2A signaling pathways. Finally, overexpression of Notch 3 has been implicated in leukemia.

Applications Reported: This HMN3-133 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HMN3-133 antibody has been tested by flow cytometric analysis of mouse thymoyctes or transfected cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

NOTCH3 is the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction (with its cell-bound ligands delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human. NOTCH3 functions as a receptor for membrane-bound ligands Jagged1, Jagged2, and Delta1 to regulate cell-fate determination. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Notch regulates Th17 differentiation and controls trafficking of IL-17 and metabolic regulators within Th17 cells in a context-dependent manner.

Scientific reports

Coutaz M,Hurrell BP,Auderset F,Wang H,Siegert S,Eberl G,Ho PC,Radtke F,Tacchini-Cottier F

12-5763 was used in Flow cytometry/Cell sorting to elucidate whether Notch signaling is involved in the negative or positive regulation of Th17 cell differentiation.