Invitrogen

Nur77 Monoclonal Antibody (12.14), PE, eBioscience™

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Nur77 Antibody (12-5965-82) in Flow

Mouse thymocytes were unstimulated (left) or stimulated for 3 hours with the Cell Stimulation Cocktail (Product # 00-4970-03) (right) then intracellularly stained with Anti-Mouse CD4 APC (Product # 17-0041-82) and 0.5 µg of Anti-Mouse Nur77 PE using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Total viable cells, as determined by Fixable Viability Dye eFluor® 506 (Product # 65-0866-14), were used for analysis.

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产品信息

12-5965-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

1 µg/test

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产品规格

种属反应

Mouse

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

12.14

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_1257209

产品详细信息

Description: This 12.14 monoclonal antibody reacts with mouse Nur77 (also known as NR4A1, TR3, NGFI-B, or NAK1), an inducible orphan nuclear receptor. Expressed in thymocytes and T cell lines, Nur77 promotes apoptosis and plays a role in thymocyte negative selection. Additionally, Nur77 has been shown to be critical for steroid biosynthesis in Leydig cells as well as for the effects of dopamine. In addition, Nur77 has been shown to interact with FoxP3 in regulatory T cells. However, our results with this antibody do not correlate with this observation.

Applications Reported: This 12.14 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This 12.14 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to Best Protocols: Protocol B: One-step protocol for intracellular (nuclear) proteins. This antibody can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

NR4A1 is a member of the steroid-thyroid hormone-retinoid receptor superfamily. NGFI-B-alpha is expressed in brain, adrenal, thyroid, liver, testis, ovary, thymus, muscle, lung and prostate. Its expression is induced in response to various stress stimuli and growth factors. The encoded protein acts as a nuclear transcription factor involved in mediating apoptotic signaling in thymocytes and tumor cells, as well as having a role in signaling in the hypothalamic-pituitary axis. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Three transcript variants encoding two distinct isoforms have been identified for this NR4A1. Variant (3), also known as TRC beta, contains multiple differences compared to variant 1. The resulting isoform b contains a shorter and distinct C-terminus compared to isoform a. RXR has been shown to be a partner for NR4A1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

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Immunity

Sugiura A,Andrejeva G,Voss K,Heintzman DR,Xu X,Madden MZ,Ye X,Beier KL,Chowdhury NU,Wolf MM,Young AC,Greenwood DL,Sewell AE,Shahi SK,Freedman SN,Cameron AM,Foerch P,Bourne T,Garcia-Canaveras JC,Karijolich J,Newcomb DC,Mangalam AK,Rabinowitz JD,Rathmell JC

12-5965-82 was used in Flow cytometry/Cell sorting to show that MTHFD2 is a metabolic checkpoint to integrate purine metabolism with pathogenic effector cell signaling and is a potential therapeutic target within 1C metabolism pathways.

Tue Jan 11 00:00:00 UTC 2022

T-bet⁺ B cells accumulate in adipose tissue and exacerbate metabolic disorder during obesity.

Cell metabolism

Hägglöf T,Vanz C,Kumagai A,Dudley E,Ortega V,Siller M,Parthasarathy R,Keegan J,Koenigs A,Shute T,Leadbetter EA

12-5965-82 was used in Flow Cytometry to reveal an important pathological role for T-bet+ B cells that should inform future immunotherapy design in type 2 diabetes and other inflammatory conditions.

Tue Aug 02 00:00:00 UTC 2022

Single-cell RNA Sequencing Identified Novel Nr4a1⁺ Ear2⁺ Anti-Inflammatory Macrophage Phenotype under Myeloid-TLR4 Dependent Regulation in Anti-Glomerular Basement Membrane (GBM) Crescentic Glomerulonephritis (cGN).

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Chen J,Huang XR,Yang F,Yiu WH,Yu X,Tang SCW,Lan HY

12-5965-82 was used in flow cytometry to study single-cell RNA sequencing (scRNA-seq), pseudotime trajectories reconstruction, and motif enrichment analysis, and macrophage diversity in anti-GBM cGN under tight regulation of myeloid-TLR4 to decode a previously unidentified role for a myeloid-TLR4 dependent Nr4a1/Ear2 negative feedback mechanism in macrophage-mediated progressive renal injury, implying that activation of Nr4a1-Ear2 axis can be a novel and effective immunotherapy for anti-GBM cGN.

Wed Jun 01 00:00:00 UTC 2022

Development of a B-cell maturation antigen-specific T-cell antigen coupler receptor for multiple myeloma.

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12-5965-82 was used in Flow cytometry/Cell sorting to represent an important step in the evolution of this technology, which tolerates a range of expression levels without impacting therapeutic efficacy.

Wed Sep 01 00:00:00 UTC 2021

T cell self-reactivity during thymic development dictates the timing of positive selection.

eLife

Lutes LK,Steier Z,McIntyre LL,Pandey S,Kaminski J,Hoover AR,Ariotti S,Streets A,Yosef N,Robey EA

12-5965-82 was used in Flow Cytometry to imply that thymocytes with low self-reactivity downregulate TCR sensitivity more slowly during positive selection, and associate membrane ion channel expression with thymocyte self-reactivity and progress through positive selection.

Thu Apr 22 00:00:00 UTC 2021

T cell self-reactivity during thymic development dictates the timing of positive selection.

eLife

Lutes LK,Steier Z,McIntyre LL,Pandey S,Kaminski J,Hoover AR,Ariotti S,Streets A,Yosef N,Robey EA

Thu Apr 22 00:00:00 UTC 2021

An Integrated Epigenomic and Transcriptomic Map of Mouse and Human αβ T Cell Development.

Immunity

Chopp LB,Gopalan V,Ciucci T,Ruchinskas A,Rae Z,Lagarde M,Gao Y,Li C,Bosticardo M,Pala F,Livak F,Kelly MC,Hannenhalli S,Bosselut R

12-5965-82 was used in Flow Cytometry to identify mouse and human αβ thymocyte developmental trajectories.

Tue Dec 15 00:00:00 UTC 2020

An Integrated Epigenomic and Transcriptomic Map of Mouse and Human αβ T Cell Development.

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Chopp LB,Gopalan V,Ciucci T,Ruchinskas A,Rae Z,Lagarde M,Gao Y,Li C,Bosticardo M,Pala F,Livak F,Kelly MC,Hannenhalli S,Bosselut R

12-5965-82 was used in Flow Cytometry to identify mouse and human αβ thymocyte developmental trajectories.

Tue Dec 15 00:00:00 UTC 2020

Dynamic regulation of chromatin organizer SATB1 via TCR-induced alternative promoter switch during T-cell development.

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Patta I,Madhok A,Khare S,Gottimukkala KP,Verma A,Giri S,Dandewad V,Seshadri V,Lal G,Misra-Sen J,Galande S

12-5965 was used in Flow cytometry/Cell sorting to study the mechanism regulating expression of SATB1 during T-cell development.

Fri Jun 19 00:00:00 UTC 2020

Dynamic regulation of chromatin organizer SATB1 via TCR-induced alternative promoter switch during T-cell development.

Nucleic acids research

Patta I,Madhok A,Khare S,Gottimukkala KP,Verma A,Giri S,Dandewad V,Seshadri V,Lal G,Misra-Sen J,Galande S

12-5965 was used in Flow cytometry/Cell sorting to study the mechanism regulating expression of SATB1 during T-cell development.

Fri Jun 19 00:00:00 UTC 2020

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12-5965 was used in Flow cytometry/Cell sorting to elucidate features of of human prenatal skin lymphocytes by using mass and flow cytometry.

Tue Nov 17 00:00:00 UTC 2020

Developing Human Skin Contains Lymphocytes Demonstrating a Memory Signature.

Cell reports. Medicine

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12-5965 was used in Flow cytometry/Cell sorting to elucidate features of of human prenatal skin lymphocytes by using mass and flow cytometry.

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Cell

Mayassi T,Ladell K,Gudjonson H,McLaren JE,Shaw DG,Tran MT,Rokicka JJ,Lawrence I,Grenier JC,van Unen V,Ciszewski C,Dimaano M,Sayegh HE,Kumar V,Wijmenga C,Green PHR,Gokhale R,Jericho H,Semrad CE,Guandalini S,Dinner AR,Kupfer SS,Reid HH,Barreiro LB,Rossjohn J,Price DA,Jabri B

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12-5965-82 was used in Flow Cytometry to identify a transcriptional program that links the CD4+ lineage with Tfh differentiation, a limiting factor for efficient B cell responses.

Tue Sep 17 00:00:00 UTC 2019

CD161 contributes to prenatal immune suppression of IFNγ-producing PLZF+ T cells.

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Halkias J,Rackaityte E,Hillman SL,Aran D,Mendoza VF,Marshall LR,MacKenzie TC,Burt TD

12-5965-82 was used in Flow cytometry/Cell sorting to reveal a fetal-specific program of protective immunity whose dysregulation is associated with fetal and neonatal inflammatory pathologies.

Thu May 30 00:00:00 UTC 2019

CD161 contributes to prenatal immune suppression of IFNγ-producing PLZF+ T cells.

The Journal of clinical investigation

Halkias J,Rackaityte E,Hillman SL,Aran D,Mendoza VF,Marshall LR,MacKenzie TC,Burt TD

12-5965-82 was used in Flow cytometry/Cell sorting to reveal a fetal-specific program of protective immunity whose dysregulation is associated with fetal and neonatal inflammatory pathologies.

Thu May 30 00:00:00 UTC 2019

Restricted Expression of the Thymoproteasome Is Required for Thymic Selection and Peripheral Homeostasis of CD8⁺ T Cells.

Cell reports

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12-5965 was used in Flow cytometry/Cell sorting to find that thymoproteasomes are essential for positive selection but that the subsequent change in peptide repertoire in the medulla is also crucial for thymic selection and that β5t-derived peptide must be confined to the thymus to avoid autoimmunity in peripheral tissues.

Tue Jan 15 00:00:00 UTC 2019

TCF1 and LEF1 Control Treg Competitive Survival and Tfr Development to Prevent Autoimmune Diseases.

Cell reports

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12-5965-82 was used in Flow Cytometry to study how CD4+ Foxp3+ T regulatory (Treg) cell differentiation and functional specification is regulated.

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12-5965 was used in Flow cytometry/Cell sorting to study TCR interaction in closely related GP33 variants.

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IgM and IgD B cell receptors differentially respond to endogenous antigens and control B cell fate.

eLife

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12-5965 was used in Flow cytometry/Cell sorting to study how IgM and IgD B cell receptors differentially respond to endogenous antigens and control B cell fate.

Fri Mar 09 00:00:00 UTC 2018

Capacity of tTreg generation is not impaired in the atrophied thymus.

PLoS biology

Oh J,Wang W,Thomas R,Su DM

Wed Nov 01 00:00:00 UTC 2017

Calcium-mediated shaping of naive CD4 T-cell phenotype and function.

eLife

Guichard V,Bonilla N,Durand A,Audemard-Verger A,Guilbert T,Martin B,Lucas B,Auffray C

12-5965 was used in Flow cytometry/Cell sorting to demonstrate that calcium-mediated activation of the calcineurin pathway acts as a rheostat to shape the phenotype and effector potential of naive CD4 T cells in the steady-state.

Thu Dec 14 00:00:00 UTC 2017

Thymic regulatory T cell niche size is dictated by limiting IL-2 from antigen-bearing dendritic cells and feedback competition.

Nature immunology

Weist BM,Kurd N,Boussier J,Chan SW,Robey EA

12-5965 was used in Flow cytometry/Cell sorting to investigate the sources of thymic IL-2 that limit access to the Treg developmental niche, showing that niche size is dictated by limiting IL-2 from antigen-bearing dendritic cells and feedback competition.

Mon Jun 01 00:00:00 UTC 2015

A sharp T-cell antigen receptor signaling threshold for T-cell proliferation.

Proceedings of the National Academy of Sciences of the United States of America

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12-5965 was used in Flow cytometry/Cell sorting to reveal a sharp threshold for the magnitude and duration of T-cell antigen receptor signalling required for commitment of T cells to proliferation.

Tue Sep 02 00:00:00 UTC 2014

Reduced TCR signaling potential impairs negative selection but does not result in autoimmune disease.

The Journal of experimental medicine

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Mon Sep 24 00:00:00 UTC 2012

生物信息学

蛋白别名: hormone receptor; MGC9485; Nuclear hormone receptor NUR/77; Nuclear protein N10; Nuclear receptor subfamily 4 group A member 1; Orphan nuclear receptor HMR; Orphan receptor tr3

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基因别名: Gfrp; GFRP1; Hbr-1; Hbr1; Hmr; N10; NGFI-B; NGFIB; NP10; Nr4a1; Nur77; TIS1; TR3

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UniProt ID: (Mouse) P12813

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Entrez Gene ID: (Mouse) 15370

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功能