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产品信息
12-9013-42
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Human,
Mouse
宿主/亚型
Mouse
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
CHI2S4N
偶联物
激发/发射光谱
565/576 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with 0.2% BSA
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2572673
产品详细信息
Description: This CHI2S4N monoclonal antibody recognizes human and mouse signal transducer and activator of transcription 6 (STAT6) when phosphorylated on tyrosine 641. Following their phosphorylation by JAKs, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine-specific manner. In response to IL-4, STAT6 is phosphorylated on tyrosine 641 by JAK1 and JAK3. STAT6 signaling downstream of the IL-4 receptor promotes T cell growth and B cell production of IgE.
Specificity of this CHI2S4N clone was determined by ELISA and flow cytometry.
Applications Reported: This CHI2S4N antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This CHI2S4N antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Staining Protocol: We recommend using Protocol C: Two-step protocol: Fixation/Methanol. Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins and Protocol B: One-step protocol: intracellular (nuclear) proteins cannot be used. All Protocols can be found in the Flow Cytometry Protocols: "Staining Intracellular Antigens for Flow Cytometry Protocol" located in the Best Protocols Section under the Resources tab online.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
STAT6 protein is a transcription factor activated by cytokines, particularly interleukin-4 and IL13. (Stat6-/-) were found to be deficient in IL-4-mediated functions including Th2 helper T-cell differentiation, expression of cell surface markers, T-cell proliferation, immunoglobulin class switching to IgE, and partial loss of IL-4 mediated proliferation. STAT6 mRNA has been detected in peripheral blood lymphocytes, colon, intestine, ovary, prostate, thymus, spleen, kidney, liver, lung and placenta. STAT6 is critically involved in Th2 immune response. STAT6 is crucial for IL-4-mediated biological responses, and naturally in two isoforms: STAT6a and STAT6c.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: 12S1644; IL-4 Stat; IL4 STAT; Signal transducer and activator of transcription 6; signal transducer and activator of transcription 6, interleukin-4 induced; Signal transducer and transcription activator 6; STAT; STAT, interleukin4-induced; STAT-6; transcription factor IL-4 STAT
基因别名: D12S1644; IL-4-STAT; STAT6; STAT6B; STAT6C
UniProt ID: (Human) P42226, (Mouse) P52633
Entrez Gene ID: (Human) 6778, (Mouse) 20852
negative regulation of transcription from RNA polymerase II promoter
T-helper 1 cell lineage commitment
negative regulation of type 2 immune response
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
signal transduction
positive regulation of type I interferon production
mammary gland epithelial cell proliferation
interleukin-4-mediated signaling pathway
regulation of cell proliferation
positive regulation of transcription from RNA polymerase II promoter
positive regulation of isotype switching to IgE isotypes
mammary gland morphogenesis
cellular response to hydrogen peroxide
cellular response to reactive nitrogen species
regulation of transcription, DNA-templated
cytokine-mediated signaling pathway
response to cytokine
