Invitrogen

TCR gamma/delta Monoclonal Antibody (B1.1), PE, eBioscience™

TCR gamma/delta Antibody in Flow Cytometry (Flow)

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TCR gamma/delta Antibody (12-9959-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD3 eFluor® 450 (Product # 48-0038-80) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human gamma delta TCR PE (right). Cells in the lymphocyte gate were used for analysis.

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产品信息

12-9959-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (4 µg)/test

查看10篇已发表文章 10篇已发表文章

功能检测 (Functional)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

B1.1

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_1603300

产品详细信息

Description: The B1.1 monoclonal antibody reacts with the human gamma delta TCR complex. The gamma delta TCR is expressed by a small subset of T cells in the thymus, peripheral lymphoid tissues, intestinal epithelium, and epidermis. The exact specificity, ligand and function of gamma delta TCR-bearing T cells are not yet fully understood; it is suggested that these cells recognize bacterial ligands and some tumor cells in the context of MHC class I-like gene products and play a role in regulation of the immune response and during bacterial infection. This monoclonal can be used as a phenotypic marker for gamma delta TCR-expressing T cells.

Applications Reported: The B1.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This B1.1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (4 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

TCR gamma/delta (T-cell receptor gamma/delta) are specialized T-cells in the immune system. The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory auto-destructive reactions and are believed to be a method by which the immune system compensates for disease. Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). However, a small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Longitudinal profiling of respiratory and systemic immune responses reveals myeloid cell-driven lung inflammation in severe COVID-19.

Immunity

Szabo PA,Dogra P,Gray JI,Wells SB,Connors TJ,Weisberg SP,Krupska I,Matsumoto R,Poon MML,Idzikowski E,Morris SE,Pasin C,Yates AJ,Ku A,Chait M,Davis-Porada J,Guo XV,Zhou J,Steinle M,Mackay S,Saqi A,Baldwin MR,Sims PA,Farber DL

Published figure using TCR gamma/delta monoclonal antibody (Product # 12-9959-42) in Flow Cytometry

Tue Apr 13 00:00:00 UTC 2021

SPARC regulation of PMN clearance protects from pristane-induced lupus and rheumatoid arthritis.

iScience

Sangaletti S,Botti L,Gulino A,Lecis D,Bassani B,Portararo P,Milani M,Cancila V,De Cecco L,Dugo M,Tripodo C,Colombo MP

12-9959-42 was used in Flow Cytometry to investigate the role of secreted protein acidic and rich in cysteine in autoimmunity using the pristane-induced model of lupus that, in mice, mimics human systemic lupus erythematosus (SLE).

Fri Jun 25 00:00:00 UTC 2021

SPARC regulation of PMN clearance protects from pristane-induced lupus and rheumatoid arthritis.

iScience

Sangaletti S,Botti L,Gulino A,Lecis D,Bassani B,Portararo P,Milani M,Cancila V,De Cecco L,Dugo M,Tripodo C,Colombo MP

Fri Jun 25 00:00:00 UTC 2021

Isolation and Quantification of Mouse γδT-cells in vitro and in vivo.

Bio-protocol

Rana I,Badarinath K,Zirmire RK,Jamora C

12-9959-42 was used in Flow cytometry/Cell sorting to describe a protocol to isolate and culture DETCs and analyse them in vivo within the skin.

Sun Sep 05 00:00:00 UTC 2021

TGF-β1 potentiates Vγ9Vδ2 T cell adoptive immunotherapy of cancer.

Cell reports. Medicine

Beatson RE,Parente-Pereira AC,Halim L,Cozzetto D,Hull C,Whilding LM,Martinez O,Taylor CA,Obajdin J,Luu Hoang KN,Draper B,Iqbal A,Hardiman T,Zabinski T,Man F,de Rosales RTM,Xie J,Aswad F,Achkova D,Joseph CR,Ciprut S,Adami A,Roider HG,Hess-Stumpp H,Győrffy B,Quist J,Grigoriadis A,Sommer A,Tutt ANJ,Davies DM,Maher J

12-9959-42 was used in Flow Cytometry to show that given their enhanced therapeutic activity and compatibility with allogeneic use, γδ[T2] cells warrant evaluation in cancer immunotherapy.

Tue Dec 21 00:00:00 UTC 2021

Systems Biology Methods Applied to Blood and Tissue for a Comprehensive Analysis of Immune Response to Hepatitis B Vaccine in Adults.

Frontiers in immunology

Ben-Othman R,Cai B,Liu AC,Varankovich N,He D,Blimkie TM,Lee AH,Gill EE,Novotny M,Aevermann B,Drissler S,Shannon CP,McCann S,Marty K,Bjornson G,Edgar RD,Lin DTS,Gladish N,Maclsaac J,Amenyogbe N,Chan Q,Llibre A,Collin J,Landais E,Le K,Reiss SM,Koff WC,Havenar-Daughton C,Heran M,Sangha B,Walt D,Krajden M,Crotty S,Sok D,Briney B,Burton DR,Duffy D,Foster LJ,Mohn WW,Kobor MS,Tebbutt SJ,Brinkman RR,Scheuermann RH,Hancock REW,Kollmann TR,Sadarangani M

12-9959 was used in Flow cytometry/Cell sorting to escribe a detailed approach that was applied in a study of 15 healthy adults, who were immunized with hepatitis B vaccine.

Mon Jun 21 00:00:00 UTC 2021

Systems Biology Methods Applied to Blood and Tissue for a Comprehensive Analysis of Immune Response to Hepatitis B Vaccine in Adults.

Frontiers in immunology

12-9959 was used in Flow cytometry/Cell sorting to escribe a detailed approach that was applied in a study of 15 healthy adults, who were immunized with hepatitis B vaccine.

Mon Jun 21 00:00:00 UTC 2021

Optimized protocols for γδ T cell expansion and lentiviral transduction.

Molecular medicine reports

Wang RN,Wen Q,He WT,Yang JH,Zhou CY,Xiong WJ,Ma L

12-9959 was used in Flow cytometry/Cell sorting to test culture protocols for γδ T cells using different combinations of isopentenyl pyrophosphate and interleukin 2.

Fri Mar 01 00:00:00 UTC 2019

Donor Cell Composition and Reactivity Predict Risk of Acute Graft-versus-Host Disease after Allogeneic Hematopoietic Stem Cell Transplantation.

Journal of immunology research

Sairafi D,Stikvoort A,Gertow J,Mattsson J,Uhlin M

12-9959 was used in Flow cytometry/Cell sorting to develop a functional assay for the assessment of individual risk for acute graft-versus-host disease.

Mon Mar 20 00:00:00 UTC 2017

Changes of cytokines during a spaceflight analog--a 45-day head-down bed rest.

PloS one

Xu X,Tan C,Li P,Zhang S,Pang X,Liu H,Li L,Sun X,Zhang Y,Wu H,Chen X,Ge Q

12-9959 was used in Flow cytometry/Cell sorting to investigate the alterations in human immune cell distributions and their functions in response to various stimuli during head-down bed rest.

Tue Jun 17 00:00:00 UTC 2014

Anti-leukemia activity of in vitro-expanded human gamma delta T cells in a xenogeneic Ph+ leukemia model.

PloS one

Siegers GM,Felizardo TC,Mathieson AM,Kosaka Y,Wang XH,Medin JA,Keating A

12-9959 was used in Flow cytometry/Cell sorting to study the effects of gamma delta T cells on the bone marrow leukaemia burden in a xenogeneic Ph+ leukemia model.

Thu Feb 03 00:00:00 UTC 2011

Gamma interferon secretion by human Vgamma2Vdelta2 T cells after stimulation with antibody against the T-cell receptor plus the Toll-Like receptor 2 agonist Pam3Cys.

Infection and immunity

Deetz CO,Hebbeler AM,Propp NA,Cairo C,Tikhonov I,Pauza CD

Tue Aug 01 00:00:00 UTC 2006

Resident Th1-like effector memory cells in pulmonary recall responses to Mycobacterium tuberculosis.

American journal of respiratory cell and molecular biology

Walrath J,Zukowski L,Krywiak A,Silver RF

Fri Jul 01 00:00:00 UTC 2005

生物信息学

蛋白别名: T3/TCR complex; TCR delta gamma; TCR gamma/delta; TCR-CD3 complex

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