Invitrogen

CD202b (TIE2) Monoclonal Antibody (TEK4), Biotin, eBioscience™

CD202b (TIE2) Antibody in Flow Cytometry (Flow)

图: 1 / 23

CD202b (TIE2) Antibody (13-5987-82) in Flow

Staining of mouse bone marrow with Anti-Mouse Ly-6A/E (Sca-1) APC (Product # 17-5981-82) and Anti-Mouse CD117 (c-Kit) APC (Product # 17-1171-82) and Anti-Mouse CD202b (TIE2) PE Total viable lineage negative cells were used for analysis.

CD202b (TIE2) Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

CD202b (TIE2) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

CD202b (TIE2) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CD202b (TIE2) Antibody in Immunohistochemistry (IHC)

Product Image Gallery

产品信息

13-5987-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

查看5篇已发表文章 5篇已发表文章

免疫组化（石蜡） (IHC (P))

查看1篇已发表文章 1篇已发表文章

免疫组化（冰冻） (IHC (F))

查看2篇已发表文章 2篇已发表文章

免疫细胞化学 (ICC/IF)

查看2篇已发表文章 2篇已发表文章

流式细胞分析 (Flow)

0.5 µg/test

查看27篇已发表文章 27篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Human, Mouse

宿主/亚型

Rat / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

TEK4

偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_466848

产品详细信息

Description: The TEK4 monoclonal antibody reacts with mouse Tie-2, also known as CD202. A member of the tyrosine kinase receptor family, Tie-2 is expressed on endothelial and a subset of hematopoietic cells and is believed to play a role in both angiogenesis and hematopoiesis during development of the mouse embryo. In fetal liver and adult bone marrow, Tie-2 is expressed by a subpopulation of hematopoietic progenitor cells characterized as Lineage markers^-, c-Kit^+, Sca1^+ cells. Long-term multilineage repopulating cells were detected in Tie-2^+, Lineage^-, c-Kit^+, Sca1^+ cells but not in Tie-2^-, Lineage^-, c-Kit^+, Sca1^+ cells.

Applications Reported: The TEK4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This TEK4 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells. TEK signaling appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis, and defects in TEK are associated with inherited venous malformations. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Immunoblotting showed that TIE2 expression was increased by thyroid-stimulating hormone and agents that increased intracellular cAMP. HSCs expressing the receptor tyrosine kinase TIE2 are quiescent and antiapoptotic and comprise a side population of HSCs that adhere to osteoblasts in the bone marrow niche.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Polycomb Bodies Detection in Murine Fibromuscular Stroma from Skin, Skeletal Muscles, and Aortic Tissues.

Methods in molecular biology (Clifton, N.J.)

Rosti V,Gorini F,Santarelli P,Sarnicola ML,Magnani S,Lanzuolo C

13-5987-82 was used in Flow cytometry/Cell sorting to combine a fluorescence-activated cell sorter (FACS)-based method with an immunofluorescence staining.

Wed May 24 00:00:00 UTC 2023

Machine Learning of Hematopoietic Stem Cell Divisions from Paired Daughter Cell Expression Profiles Reveals Effects of Aging on Self-Renewal.

Cell systems

Arai F,Stumpf PS,Ikushima YM,Hosokawa K,Roch A,Lutolf MP,Suda T,MacArthur BD

13-5987-82 was used in Flow Cytometry to indicate that the balance between intrinsic and extrinsic regulation of stem cell activity alters substantially with age and help explain why stem cell numbers increase through life, yet regenerative potency declines.

Wed Dec 16 00:00:00 UTC 2020

A RUNX2 stabilization pathway mediates physiologic and pathologic bone formation.

Nature communications

Kim JM,Yang YS,Park KH,Ge X,Xu R,Li N,Song M,Chun H,Bok S,Charles JF,Filhol-Cochet O,Boldyreff B,Dinter T,Yu PB,Kon N,Gu W,Takarada T,Greenblatt MB,Shim JH

13-5987 was used in Flow cytometry/Cell sorting to demonstrate that CK2-induced phosphorylation of RUNX2 recruits the deubiquitinase HAUSP for the stabilization of RUNX2 and that this pathway is required for physiologic and pathologic bone formation.

Fri May 08 00:00:00 UTC 2020

A RUNX2 stabilization pathway mediates physiologic and pathologic bone formation.

Nature communications

Kim JM,Yang YS,Park KH,Ge X,Xu R,Li N,Song M,Chun H,Bok S,Charles JF,Filhol-Cochet O,Boldyreff B,Dinter T,Yu PB,Kon N,Gu W,Takarada T,Greenblatt MB,Shim JH

Fri May 08 00:00:00 UTC 2020

Prrx1 Fibroblasts Represent a Pro-fibrotic Lineage in the Mouse Ventral Dermis.

Cell reports

Leavitt T,Hu MS,Borrelli MR,Januszyk M,Garcia JT,Ransom RC,Mascharak S,desJardins-Park HE,Litzenburger UM,Walmsley GG,Marshall CD,Moore AL,Duoto B,Adem S,Foster DS,Salhotra A,Shen AH,Griffin M,Shen EZ,Barnes LA,Zielins ER,Maan ZN,Wei Y,Chan CKF,Wan DC,Lorenz HP,Chang HY,Gurtner GC,Longaker MT

13-5987-82 was used in Flow Cytometry to identify and characterise a fibroblast subpopulation in the mouse ventral dermis with intrinsic scar-forming potential.

Tue Nov 10 00:00:00 UTC 2020

Mesenchymal VEGFA induces aberrant differentiation in heterotopic ossification.

Bone research

Hwang C,Marini S,Huber AK,Stepien DM,Sorkin M,Loder S,Pagani CA,Li J,Visser ND,Vasquez K,Garada MA,Li S,Xu J,Hsu CY,Yu PB,James AW,Mishina Y,Agarwal S,Li J,Levi B

Published figure using CD202b (TIE2) monoclonal antibody (Product # 13-5987-82) in Flow Cytometry

Sun Oct 17 00:00:00 UTC 2021

Protumoral bone marrow-derived cells migrate via Gβγ-dependent signaling pathways and exhibit a complex repertoire of RhoGEFs.

Journal of cell communication and signaling

Cervantes-Villagrana RD,Color-Aparicio VM,Reyes-Cruz G,Vázquez-Prado J

Published figure using CD202b (TIE2) monoclonal antibody (Product # 13-5987-82) in Flow Cytometry

Sat Jun 01 00:00:00 UTC 2019

Robust hematopoietic specification requires the ubiquitous Sp1 and Sp3 transcription factors.

Epigenetics & chromatin

Gilmour J,O'Connor L,Middleton CP,Keane P,Gillemans N,Cazier JB,Philipsen S,Bonifer C

Published figure using CD202b (TIE2) monoclonal antibody (Product # 13-5987-82) in Flow Cytometry

Tue Jun 04 00:00:00 UTC 2019

Isolation and functional assessment of mouse skeletal stem cell lineage.

Nature protocols

Gulati GS,Murphy MP,Marecic O,Lopez M,Brewer RE,Koepke LS,Manjunath A,Ransom RC,Salhotra A,Weissman IL,Longaker MT,Chan CKF

Published figure using CD202b (TIE2) monoclonal antibody (Product # 13-5987-82) in Flow Cytometry

Fri Jun 01 00:00:00 UTC 2018

Macrophages orchestrate breast cancer early dissemination and metastasis.

Nature communications

Linde N,Casanova-Acebes M,Sosa MS,Mortha A,Rahman A,Farias E,Harper K,Tardio E,Reyes Torres I,Jones J,Condeelis J,Merad M,Aguirre-Ghiso JA

13-5987 was used in Flow cytometry/Cell sorting to show that, in pre-malignant lesions, CCL2 produced by cancer cells and myeloid cells attracts CD206+/Tie2+macrophages and induces Wnt-1 upregulation that in turn downregulates E-cadherin junctions in the HER2+early cancer cells.

Tue Jan 02 00:00:00 UTC 2018

Neoadjuvant chemotherapy induces breast cancer metastasis through a TMEM-mediated mechanism.

Science translational medicine

Karagiannis GS,Pastoriza JM,Wang Y,Harney AS,Entenberg D,Pignatelli J,Sharma VP,Xue EA,Cheng E,D'Alfonso TM,Jones JG,Anampa J,Rohan TE,Sparano JA,Condeelis JS,Oktay MH

Published figure using CD202b (TIE2) monoclonal antibody (Product # 13-5987-82) in Immunohistochemistry

Wed Jul 05 00:00:00 UTC 2017

Neoadjuvant chemotherapy induces breast cancer metastasis through a TMEM-mediated mechanism.

Science translational medicine

Karagiannis GS,Pastoriza JM,Wang Y,Harney AS,Entenberg D,Pignatelli J,Sharma VP,Xue EA,Cheng E,D'Alfonso TM,Jones JG,Anampa J,Rohan TE,Sparano JA,Condeelis JS,Oktay MH

Published figure using CD202b (TIE2) monoclonal antibody (Product # 13-5987-82) in Immunohistochemistry

Wed Jul 05 00:00:00 UTC 2017

The Selective Tie2 Inhibitor Rebastinib Blocks Recruitment and Function of Tie2^Hi Macrophages in Breast Cancer and Pancreatic Neuroendocrine Tumors.

Molecular cancer therapeutics

Harney AS,Karagiannis GS,Pignatelli J,Smith BD,Kadioglu E,Wise SC,Hood MM,Kaufman MD,Leary CB,Lu WP,Al-Ani G,Chen X,Entenberg D,Oktay MH,Wang Y,Chun L,De Palma M,Jones JG,Flynn DL,Condeelis JS

Published figure using CD202b (TIE2) monoclonal antibody (Product # 13-5987-82) in Immunofluorescence

Wed Nov 01 00:00:00 UTC 2017

Pericyte-expressed Tie2 controls angiogenesis and vessel maturation.

Nature communications

Teichert M,Milde L,Holm A,Stanicek L,Gengenbacher N,Savant S,Ruckdeschel T,Hasanov Z,Srivastava K,Hu J,Hertel S,Bartol A,Schlereth K,Augustin HG

Published figure using CD202b (TIE2) monoclonal antibody (Product # 13-5987-82) in Flow Cytometry

Tue Jul 18 00:00:00 UTC 2017

Primitive erythrocytes are generated from hemogenic endothelial cells.

Scientific reports

Stefanska M,Batta K,Patel R,Florkowska M,Kouskoff V,Lacaud G

13-5987 was used in Immunohistochemistry to demonstrate that both in vivo and in vitro primitive erythrocytes are generated from hemogenic endothelial cells.

Tue Jul 25 00:00:00 UTC 2017

Primitive erythrocytes are generated from hemogenic endothelial cells.

Scientific reports

Stefanska M,Batta K,Patel R,Florkowska M,Kouskoff V,Lacaud G

13-5987 was used in Immunohistochemistry to demonstrate that both in vivo and in vitro primitive erythrocytes are generated from hemogenic endothelial cells.

Tue Jul 25 00:00:00 UTC 2017

Lymphatic Contribution to the Cellular Niche in Heterotopic Ossification.

Annals of surgery

Loder S,Agarwal S,Sorkin M,Breuler C,Li J,Peterson J,Gardenier J,Hsieh HH,Wang SC,Mehrara BJ,Levi B

Published figure using CD202b (TIE2) monoclonal antibody (Product # 13-5987-82) in Flow Cytometry

Thu Dec 01 00:00:00 UTC 2016

Dynamic Gene Regulatory Networks Drive Hematopoietic Specification and Differentiation.

Developmental cell

Goode DK,Obier N,Vijayabaskar MS,Lie-A-Ling M,Lilly AJ,Hannah R,Lichtinger M,Batta K,Florkowska M,Patel R,Challinor M,Wallace K,Gilmour J,Assi SA,Cauchy P,Hoogenkamp M,Westhead DR,Lacaud G,Kouskoff V,Göttgens B,Bonifer C

Published figure using CD202b (TIE2) monoclonal antibody (Product # 13-5987-82) in Immunofluorescence

Mon Mar 07 00:00:00 UTC 2016

ECM-Dependence of Endothelial Progenitor Cell Features.

Journal of cellular biochemistry

Siavashi V,Nassiri SM,Rahbarghazi R,Vafaei R,Sariri R

Published figure using CD202b (TIE2) monoclonal antibody (Product # 13-5987-82) in Flow Cytometry

Mon Aug 01 00:00:00 UTC 2016

Endoglin potentiates nitric oxide synthesis to enhance definitive hematopoiesis.

Biology open

Nasrallah R,Knezevic K,Thai T,Thomas SR,Göttgens B,Lacaud G,Kouskoff V,Pimanda JE

13598782 was used in flow cytometry to investigate the role of endoglin in the development of hematopoietic cells.

Fri May 15 00:00:00 UTC 2015

The transcription factor Mesp1 interacts with cAMP-responsive element binding protein 1 (Creb1) and coactivates Ets variant 2 (Etv2) gene expression.

The Journal of biological chemistry

Shi X,Zirbes KM,Rasmussen TL,Ferdous A,Garry MG,Koyano-Nakagawa N,Garry DJ

Published figure using CD202b (TIE2) monoclonal antibody (Product # 13-5987-82) in Immunohistochemistry

Fri Apr 10 00:00:00 UTC 2015

A crucial role for the ubiquitously expressed transcription factor Sp1 at early stages of hematopoietic specification.

Development (Cambridge, England)

Gilmour J,Assi SA,Jaegle U,Kulu D,van de Werken H,Clarke D,Westhead DR,Philipsen S,Bonifer C

Published figure using CD202b (TIE2) monoclonal antibody (Product # 13-5987-82) in Flow Cytometry

Sun Jun 01 00:00:00 UTC 2014

Cooperative interaction of Etv2 and Gata2 regulates the development of endothelial and hematopoietic lineages.

Developmental biology

Shi X,Richard J,Zirbes KM,Gong W,Lin G,Kyba M,Thomson JA,Koyano-Nakagawa N,Garry DJ

Published figure using CD202b (TIE2) monoclonal antibody (Product # 13-5987-82) in Flow Cytometry

Thu May 15 00:00:00 UTC 2014

Ectopic Runx1 expression rescues Tal-1-deficiency in the generation of primitive and definitive hematopoiesis.

PloS one

Tornack J,Seiler K,Grützkau A,Grün JR,Onodera M,Melchers F,Tsuneto M

13-5987 was used in Flow cytometry/Cell sorting to suggest that Tal-1 expression is needed to express Runx1 in the mesoderm.

Thu Aug 21 00:00:00 UTC 2014

GFI1 and GFI1B control the loss of endothelial identity of hemogenic endothelium during hematopoietic commitment.

Blood

Lancrin C,Mazan M,Stefanska M,Patel R,Lichtinger M,Costa G,Vargel O,Wilson NK,Möröy T,Bonifer C,Göttgens B,Kouskoff V,Lacaud G

13-5987 was used in Flow cytometry/Cell sorting to demonstrate a critical and specific role of the GFI1 transcription factors in the first steps of the process leading to the generation of hematopoietic progenitors.

Thu Jul 12 00:00:00 UTC 2012

GFI1 and GFI1B control the loss of endothelial identity of hemogenic endothelium during hematopoietic commitment.

Blood

Lancrin C,Mazan M,Stefanska M,Patel R,Lichtinger M,Costa G,Vargel O,Wilson NK,Möröy T,Bonifer C,Göttgens B,Kouskoff V,Lacaud G

Thu Jul 12 00:00:00 UTC 2012

Etv2 is expressed in the yolk sac hematopoietic and endothelial progenitors and regulates Lmo2 gene expression.

Stem cells (Dayton, Ohio)

Koyano-Nakagawa N,Kweon J,Iacovino M,Shi X,Rasmussen TL,Borges L,Zirbes KM,Li T,Perlingeiro RC,Kyba M,Garry DJ

Wed Aug 01 00:00:00 UTC 2012

Etv2 is expressed in the yolk sac hematopoietic and endothelial progenitors and regulates Lmo2 gene expression.

Stem cells (Dayton, Ohio)

Koyano-Nakagawa N,Kweon J,Iacovino M,Shi X,Rasmussen TL,Borges L,Zirbes KM,Li T,Perlingeiro RC,Kyba M,Garry DJ

Wed Aug 01 00:00:00 UTC 2012

Etv2 is expressed in the yolk sac hematopoietic and endothelial progenitors and regulates Lmo2 gene expression.

Stem cells (Dayton, Ohio)

Koyano-Nakagawa N,Kweon J,Iacovino M,Shi X,Rasmussen TL,Borges L,Zirbes KM,Li T,Perlingeiro RC,Kyba M,Garry DJ

Wed Aug 01 00:00:00 UTC 2012

The notch ligand delta-like 4 regulates multiple stages of early hemato-vascular development.

PloS one

Laranjeiro R,Alcobia I,Neves H,Gomes AC,Saavedra P,Carvalho CC,Duarte A,Cidadão A,Parreira L

13-5987 was used in Flow cytometry/Cell sorting to indicate a role for the notch ligand delta-like 4 in the quantitative regulation of early hemato-vascular precursors.

Thu Aug 30 00:00:00 UTC 2012

Paired immunoglobin-like receptor-B regulates the suppressive function and fate of myeloid-derived suppressor cells.

Immunity

Ma G,Pan PY,Eisenstein S,Divino CM,Lowell CA,Takai T,Chen SH

13-5987 was used in Flow cytometry/Cell sorting to examine the role of paired immunoglobulin-like receptor B (PIR-B) in tumour-induced immunity.

Fri Mar 25 00:00:00 UTC 2011

ER71 directs mesodermal fate decisions during embryogenesis.

Development (Cambridge, England)

Rasmussen TL,Kweon J,Diekmann MA,Belema-Bedada F,Song Q,Bowlin K,Shi X,Ferdous A,Li T,Kyba M,Metzger JM,Koyano-Nakagawa N,Garry DJ

Tue Nov 01 00:00:00 UTC 2011

ER71 directs mesodermal fate decisions during embryogenesis.

Development (Cambridge, England)

Rasmussen TL,Kweon J,Diekmann MA,Belema-Bedada F,Song Q,Bowlin K,Shi X,Ferdous A,Li T,Kyba M,Metzger JM,Koyano-Nakagawa N,Garry DJ

Tue Nov 01 00:00:00 UTC 2011

The haemangioblast generates haematopoietic cells through a haemogenic endothelium stage.

Nature

Lancrin C,Sroczynska P,Stephenson C,Allen T,Kouskoff V,Lacaud G

13-5987 was used in Flow cytometry/Cell sorting to merge the two a priori conflicting theories on the origin of haematopoietic development into a single linear developmental process.

Thu Feb 12 00:00:00 UTC 2009

Low levels of tumor necrosis factor alpha increase tumor growth by inducing an endothelial phenotype of monocytes recruited to the tumor site.

Cancer research

Li B,Vincent A,Cates J,Brantley-Sieders DM,Polk DB,Young PP

13-5987 was used in Flow cytometry/Cell sorting to investigate the role of TNF-alpha in endothelial differentiation of myeloid progenitors in tumours.

Thu Jan 01 00:00:00 UTC 2009

Mapping of angiogenic markers for targeting of vectors to tumor vascular endothelial cells.

Cancer gene therapy

Tang Y,Borgstrom P,Maynard J,Koziol J,Hu Z,Garen A,Deisseroth A

Sun Apr 01 00:00:00 UTC 2007

Isolation of cardiac cells from E8.5 yolk sac by ALCAM (CD166) expression.

Mechanisms of development

Murakami Y,Hirata H,Miyamoto Y,Nagahashi A,Sawa Y,Jakt M,Asahara T,Kawamata S

13-5987 was used in Flow cytometry/Cell sorting to indicate that cells retaining a potential to differentiate to the cardiac lineage are present in E8.5 yolk sacs and can be isolated as ALCAM high+, Flk-1- cells.

Wed Mar 19 00:00:00 UTC 2008

Differential expression of novel potential regulators in hematopoietic stem cells.

PLoS genetics

Forsberg EC,Prohaska SS,Katzman S,Heffner GC,Stuart JM,Weissman IL

13-5987 was used in Flow cytometry/Cell sorting to compare the expression pattern of three hematopoietic subpopulations in adult mouse bone marrow.

Thu Sep 01 00:00:00 UTC 2005

Postnatal bone marrow stromal cells elicit a potent VEGF-dependent neoangiogenic response in vivo.

Gene therapy

Al-Khaldi A,Eliopoulos N,Martineau D,Lejeune L,Lachapelle K,Galipeau J

13-5987 was used in Flow cytometry/Cell sorting to determine the functional neoangiogenic potential of bone marrow stromal cells in vivo.

Tue Apr 01 00:00:00 UTC 2003

生物信息学

蛋白别名: Angiopoietin-1 receptor; CD202b; CD202b antigen; EC 2.7.10; EC 2.7.10.1; Endothelial tyrosine kinase; HYK; mTIE2; p140 TEK; STK1; TEKT1; Tektin-1; Tunica interna endothelial cell kinase; Tyrosine kinase with Ig and EGF homology domains-2; Tyrosine-protein kinase receptor TEK; Tyrosine-protein kinase receptor TIE-2

查看更多收起

基因别名: AA517024; Cd202b; Hyk; STK1; Tek; Tie-2; Tie2

查看更多收起

UniProt ID: (Mouse) Q02858

查看更多收起

Entrez Gene ID: (Mouse) 21687

查看更多收起

功能