Invitrogen

Kappa Light Chain Monoclonal Antibody (TB28-2), Biotin, eBioscience™

Kappa Light Chain Antibody in Flow Cytometry (Flow)

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Kappa Light Chain Antibody (13-9970-82) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD19 APC (Product # 17-0199-42) and 0.125 µg of Mouse IgG1 k Isotype Control Biotin (Product # 13-4714-85) (left) or 0.125 µg of Anti-Human Ig kappa Light Chain Biotin (right) followed by Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis.

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产品信息

13-9970-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.25 µg/test

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

TB28-2

偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_1907419

产品详细信息

Description: The monoclonal antibody TB28-2 recognizes the kappa light chain of human immunoglobulin. Approximately 22-kDa, the Ig kappa chain is comprised of one variable and one constant region. Two kappa chains together with the heavy chain comprise the whole Ig structure. Expression of Ig kappa is typically found on more than half of B lymphocytes.

Applications Reported: This TB28-2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This TB28-2 antibody has been tested by flow cytometric analysis of normal peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five types) and two light chains (kappa, lambda; both having a molecular weight of 22.5 kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30. Antibodies to the kappa light chain are reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

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