Invitrogen

CD9 Monoclonal Antibody (eBioSN4 (SN4 C3-3A2)), eBioscience™

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CD9 Antibody (14-0098-82) in Flow

Staining of normal human peripheral blood cells with 0.25 µg of Mouse IgG11 K Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.25 µg of Anti-Human CD9 Purified (filled histogram) followed by Anti-Mouse IgG FITC (Product # 11-4011-85). Total viable cells were used for analysis.

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产品信息

14-0098-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

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免疫组化（冰冻） (IHC (F))

Assay-Dependent

流式细胞分析 (Flow)

0.5 µg/test

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免疫沉淀 (IP)

Assay-Dependent

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

eBioSN4 (SN4 C3-3A2)

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_657777

产品详细信息

Description: The eBioSN4 monoclonal antibody reacts with human CD9. CD9 is a 24 kDa member of the tetraspanin family, whose members are characterized by the presence of 4 hydrophobic transmembrane domains. CD9 is expressed in platelets, eosinophils, basophils, pre-B cells, activated T cells and neural cell lines. Furthermore, CD9 expression has been associated with a malignant phenotype, including expression on 90% of non T cell acute lymphoblastic leukemia cells and on 50% of chronic lymphocytic and acute myeloblastic leukemias. In platelets, CD9 is expressed in alpha-granules and through association with the integrin alphaIIb/betaIII plays a role in platelet aggregation, as demonstrated by the use of anti-human CD9 antibodies. CD9 has also been shown to induce the aggregation of pre-B cell lines, and the adhesion and migration of pre-B cells and Schwann cells. Additionally, it has been demonstrated that CD9 is able to provide a co-stimulatory signal for T cells independently of CD28, in the absence of antigen-presenting cells. Binding of the eBioSN4 monoclonal antibody partially cross-blocks binding of another anti-human CD9 monoclonal antibody, MM2/57.

Applications Reported: This eBioSN4 (SN4 C3-3A2) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections.

Applications Tested: This eBioSN4 (SN4 C3-3A2) antibody has been tested by flow cytometric analysis of normal human platelets. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. CD9 belongs to a family of membrane proteins termed tetraspanins which transverse the membrane four times. In pre B cells and platelets, CD9 antigen regulates cell activation and aggregation possibly through an association with the integrin CD41 / CD61 (GPIIb / GPIIIa). CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression plays a critical role in the suppression of cancer cell motility and metastasis. CD9 is also considered as metastasis suppressor in solid tumors.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Extracellular vesicles as a potential source of tumor-derived DNA in advanced pancreatic cancer.

PloS one

Lapin M,Tjensvoll K,Nedrebø K,Taksdal E,Janssen H,Gilje B,Nordgård O

14-0098-82 was used in Western Blot to suggest that quantification of evDNA, as a source of tumor-derived DNA, does not add information to that obtained with cfDNA, at least not in patients with advanced pancreatic cancer.

Mon Sep 18 00:00:00 UTC 2023

Extracellular vesicles as a potential source of tumor-derived DNA in advanced pancreatic cancer.

PloS one

Lapin M,Tjensvoll K,Nedrebø K,Taksdal E,Janssen H,Gilje B,Nordgård O

Mon Sep 18 00:00:00 UTC 2023

Stem cell-derived exosomes from human exfoliated deciduous teeth promote angiogenesis in hyperglycemic-induced human umbilical vein endothelial cells.

Journal of applied oral science : revista FOB

Sunartvanichkul T,Arayapisit T,Sangkhamanee SS,Chaweewannakorn C,Iwasaki K,Klaihmon P,Sritanaudomchai H

Published figure using CD9 monoclonal antibody (Product # 14-0098-82) in Flow Cytometry

Fri Apr 14 00:00:00 UTC 2023

Anti-Human CD9 Fab Fragment Antibody Blocks the Extracellular Vesicle-Mediated Increase in Malignancy of Colon Cancer Cells.

Cells

Santos MF,Rappa G,Fontana S,Karbanová J,Aalam F,Tai D,Li Z,Pucci M,Alessandro R,Morimoto C,Corbeil D,Lorico A

Published figure using CD9 monoclonal antibody (Product # 14-0098-82) in Flow Cytometry

Wed Aug 10 00:00:00 UTC 2022

Extracellular Vesicle Membrane Protein Profiling and Targeted Mass Spectrometry Unveil CD59 and Tetraspanin 9 as Novel Plasma Biomarkers for Detection of Colorectal Cancer.

Cancers

Dash S,Wu CC,Wu CC,Chiang SF,Lu YT,Yeh CY,You JF,Chu LJ,Yeh TS,Yu JS

Published figure using CD9 monoclonal antibody (Product # 14-0098-82) in Flow Cytometry

Wed Dec 28 00:00:00 UTC 2022

CD9 knockdown suppresses cell proliferation, adhesion, migration and invasion, while promoting apoptosis and the efficacy of chemotherapeutic drugs and imatinib in Ph+ ALL SUP‑B15 cells.

Molecular medicine reports

Xing C,Xu W,Shi Y,Zhou B,Wu D,Liang B,Zhou Y,Gao S,Feng J

Published figure using CD9 monoclonal antibody (Product # 14-0098-82) in Flow Cytometry

Thu Oct 01 00:00:00 UTC 2020

Systems-Level Immunomonitoring from Acute to Recovery Phase of Severe COVID-19.

Cell reports. Medicine

Rodriguez L,Pekkarinen PT,Lakshmikanth T,Tan Z,Consiglio CR,Pou C,Chen Y,Mugabo CH,Nguyen NA,Nowlan K,Strandin T,Levanov L,Mikes J,Wang J,Kantele A,Hepojoki J,Vapalahti O,Heinonen S,Kekäläinen E,Brodin P

14-0098 was used in Flow cytometry/Cell sorting to report on a systems-level blood immunomonitoring study of 37 adult patients diagnosed with COVID-19 and followed with up to 14 blood samples from acute to recovery phases of the disease.

Tue Aug 25 00:00:00 UTC 2020

Stereotypic Immune System Development in Newborn Children.

Cell

Olin A,Henckel E,Chen Y,Lakshmikanth T,Pou C,Mikes J,Gustafsson A,Bernhardsson AK,Zhang C,Bohlin K,Brodin P

14-0098 was used in Flow cytometry/Cell sorting to study stereotypic immune system development in newborn children.

Thu Aug 23 00:00:00 UTC 2018

Circulating exosomes suppress the induction of regulatory T cells via let-7i in multiple sclerosis.

Nature communications

Kimura K,Hohjoh H,Fukuoka M,Sato W,Oki S,Tomi C,Yamaguchi H,Kondo T,Takahashi R,Yamamura T

14-0098 was used in Western Blotting to show that exosomal let-7i regulates MS pathogenesis by blocking the IGF1R/TGFBR1 pathway.

Tue Jan 02 00:00:00 UTC 2018

Human islets contain four distinct subtypes of β cells.

Nature communications

Dorrell C,Schug J,Canaday PS,Russ HA,Tarlow BD,Grompe MT,Horton T,Hebrok M,Streeter PR,Kaestner KH,Grompe M

Published figure using CD9 monoclonal antibody (Product # 14-0098-82) in Flow Cytometry

Mon Jul 11 00:00:00 UTC 2016

Microvesicles derived from human umbilical cord Wharton's jelly mesenchymal stem cells attenuate bladder tumor cell growth in vitro and in vivo.

PloS one

Wu S,Ju GQ,Du T,Zhu YJ,Liu GH

Published figure using CD9 monoclonal antibody (Product # 14-0098-82) in Flow Cytometry

Tue Oct 22 00:00:00 UTC 2013

生物信息学

蛋白别名: 5H9; 5H9 antigen; antigen CD9; BA-2/p24 antigen; BA2; CD9; CD9 antigen; CD9 antigen (p24); Cell growth-inhibiting gene 2 protein; Leukocyte antigen MIC3; motility related protein-1; Motility-related protein; MRP-1; p24; sCD 9; sCD9; soluble CD 9; soluble CD9; Tetraspanin-29; Tetraspanin29; Tspan-29

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基因别名: BTCC-1; CD9; DRAP-27; GIG2; MIC3; MRP-1; TSPAN-29; TSPAN29

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UniProt ID: (Human) P21926

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Entrez Gene ID: (Human) 928

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功能

integrin binding protein binding

参与通路