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14-3-3 Pan Antibody (51-0700) in ICC/IF
Immunofluorescence analysis of 14-3-3 theta was performed using 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with 14-3-3 theta Rabbit Polyclonal Antibody (Product # 51-0700) at 2 µg/mL in 0.1% BSA and incubated for 3 hours ... View More
产品信息
51-0700
应用
建议稀释比
已发表文章
产品规格
种属反应
Bovine,
Human,
Mouse,
Rat,
Xenopus
已发表种属
Bovine,
Fruit fly,
Human,
Mouse,
Plant
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A 20 amino acid synthetic peptide derived from the N-terminus of the human 14-3-3 beta/alpha protein.
偶联物
形式
Liquid
浓度
0.25 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
0.1% sodium azide
保存条件
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2533894
产品详细信息
This antibody is broadly reactive with members of the 14-3-3 family of proteins (~29-32 kDa). With the exception of a single conserved amino acid change, this sequence is identical in the delta and zeta isoforms. Three and four conserved amino acid differences are found in the tau and gamma isoforms, respectively.
Based on the sequence homology to the immunizing peptide, this the antibody could recognize the protein from C. elegans.
靶标信息
At least seven isoforms comprise the highly conserved 14-3-3 family of homo- and heterodimeric proteins that are abundantly expressed in all eukaryotic cells. Although more than seven isoforms of 14-3-3 have been described, some redundancies have appeared upon sequencing. The 14-3-3s are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins. By interacting with Cdc25C, 14-3-3 regulates entry into the cell cycle and, through interaction with Bad, prevents apoptosis. Other proteins that have been shown to bind to 14-3-3s include members of the protein kinase C family, Cbl, IRS-1, polyoma middle-T antigen, nitrate reductase, S-raf and the IGF-1 receptor. Detection of 14-3-3 proteins in cerebrospinal fluid has been shown to be quite useful in the differential diagnosis of Creutzfeldt-Jakob disease and other prion diseases.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 14-3-3 alpha; 14-3-3 beta; 14-3-3 delta; 14-3-3 epsilon; 14-3-3 gamma; 14-3-3 protein beta; 14-3-3 protein beta-subtype; 14-3-3 protein beta/alpha; 14-3-3 protein beta/alpha-A; 14-3-3 protein beta/alpha-B; 14-3-3 protein epsilon; 14-3-3 protein gamma; 14-3-3 protein gamma subtype; 14-3-3 protein gamma-A; 14-3-3 protein gamma-B; 14-3-3 protein gamma-subtype; 14-3-3 protein sigma; 14-3-3 protein T-cell; 14-3-3 protein tau; 14-3-3 protein theta; 14-3-3 protein zeta; 14-3-3 protein zeta/delta; 14-3-3 protein/cytosolic phospholipase A2; 14-3-3 sigma; 14-3-3 sigma protein; 14-3-3 tau; 14-3-3 theta; 14-3-3 zeta; 14-3-3d; 14-3-3E; 3-monooxgenase/tryptophan 5-monooxgenase activation protein, gamma polypeptide; 3-monooxgenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide; 3-monooxygenase/tryptophan 5 monooxygenase activation protein beta polypeptide; 3-monooxygenase/tryptophan 5-monooxgenase activation protein gamma polypeptide; 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide; brain protein 14-3-3, beta isoform; epididymis luminal protein 2; epididymis luminal protein 4; epididymis secretory protein Li 1; epididymis secretory protein Li 3; epididymis secretory protein Li 93; Epithelial cell marker protein 1; Factor activating exoenzyme S; FAS; KCIP-1; mitochondrial import stimulation factor (MSF) L subunit; Mitochondrial import stimulation factor L subunit; Mitochondrial import stimulation factor S1 subunit; MSF L; phospholipase A2; Prepronerve growth factor RNH-1; Protein 1054; Protein HS1; Protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; protein phosphatase 1, regulatory subunit 170; protein tau; protein, theta; SEZ-2; Stratifin; tryosine 3-monooxgenase/tryptophan 5 monooxgenase activation protein gamma; tryosine 3-monooxgenase/tryptophan 5-monooxgenase activation protein gamma polypeptide; tryosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta polypeptide; tryosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma; tyrosine 3-monooxgenase/tryptophan 5 monooxgenase activation protein beta polypeptide; tyrosine 3-monooxgenase/tryptophan 5 monooxgenase activation protein, beta polypeptide; tyrosine 3-monooxgenase/tryptophan 5-monooxgenase activation protein, beta polypeptide; tyrosine 3-monooxgenase/tryptophan 5-monooxgenase activation protein, gamma polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alpha polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, delta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, sigma polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activatioprotein epsilon polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activatioprotein, epsilon polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein beta polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein gamma polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, beta polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, epsilon polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, gamma polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, theta polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, zeta polypeptide
基因别名: 1110013I11Rik; 1300003C17Rik; 14-3-3; 14-3-3-zeta; 14-3-3beta; 14-3-3E; 14-3-3G; 14-3-3GAMMA; 14-3-3t; 14-3-3z; 14-3-3zeta; 1C5; 2700028P07Rik; AA409740; AI596267; AL022924; AU019196; AU020854; AU021156; D7Bwg1348e; Er; GW128; HEL-S-1; HEL-S-3; HEL-S-93; HEL2; HEL4; HME1; HS1; KCIP-1; MDCR; MDS; Mkrn3; Mme1; Msfs1; PPP1R170; R74690; SFN; XELAEV_18012695mg; XELAEV_18015318mg; XELAEV_18043238mg; XELAEV_18045999mg; YWHAA; YWHAB; ywhab-a; ywhab-b; YWHAD; YWHAE; YWHAG; ywhag-a; ywhag-b; YWHAQ; YWHAS; YWHAZ
UniProt ID: (Bovine) Q0VC36, (Human) P31947, (Mouse) O70456, (Bovine) P68250, (Human) P31946, (Bovine) P62261, (Human) P62258, (Human) P61981, (Human) P27348, (Bovine) Q3SZI4, (Human) P63104, (Bovine) P63103, (Mouse) Q9CQV8, (Rat) P35213, (Mouse) P62259, (Rat) P62260, (Mouse) P61982, (Rat) P61983, (Rat) P68255, (Mouse) P68254, (Mouse) P63101, (Rat) P63102, (Xenopus) Q5XHK2, (Xenopus) Q8AVQ3, (Xenopus) Q6PCG0, (Xenopus) Q6NRY9
Entrez Gene ID: (Bovine) 528453, (Human) 2810, (Mouse) 55948, (Rat) 313017, (Bovine) 286863, (Human) 7529, (Bovine) 282125, (Human) 7531, (Human) 7532, (Bovine) 286862, (Human) 10971, (Bovine) 768311, (Human) 7534, (Bovine) 287022, (Mouse) 54401, (Rat) 56011, (Mouse) 22627, (Rat) 29753, (Mouse) 22628, (Rat) 56010, (Rat) 25577, (Mouse) 22630, (Mouse) 22631, (Rat) 25578, (Xenopus) 399276, (Xenopus) 380457, (Xenopus) 379724, (Xenopus) 432005, (Xenopus) 100505434
protein kinase binding
identical protein binding
phosphoprotein binding
protein binding
protein kinase C inhibitor activity
protein domain specific binding
cadherin binding involved in cell-cell adhesion
molecular_function
transcription corepressor activity
protein C-terminus binding
enzyme binding
protein complex binding
histone deacetylase binding
phosphoserine binding
potassium channel regulator activity
MHC class II protein complex binding
ubiquitin protein ligase binding
ion channel binding
poly(A) RNA binding
protein heterodimerization activity
protein kinase C binding
insulin-like growth factor receptor binding
receptor tyrosine kinase binding
protein N-terminus binding
14-3-3 protein binding
transcription factor binding
actin binding
enzyme regulator activity
scaffold/adaptor protein
transporter
regulation of cyclin-dependent protein serine/threonine kinase activity
release of cytochrome c from mitochondria
keratinocyte development
intrinsic apoptotic signaling pathway in response to DNA damage
regulation of epidermal cell division
negative regulation of keratinocyte proliferation
positive regulation of cell growth
keratinization
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of epidermal cell differentiation
positive regulation of protein export from nucleus
establishment of skin barrier
negative regulation of protein kinase activity
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
signal transduction
membrane organization
negative regulation of protein serine/threonine kinase activity
cell-cell adhesion
positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
keratinocyte differentiation
skin development
regulation of cell cycle
biological_process
protein targeting
negative regulation of G-protein coupled receptor protein signaling pathway
MAPK cascade
viral process
negative regulation of protein dephosphorylation
hippo signaling
positive regulation of catalytic activity
regulation of mRNA stability
negative regulation of transcription, DNA-templated
cytoplasmic sequestering of protein
protein heterooligomerization
protein localization to nucleus
cellular response to heat
G2/M transition of mitotic cell cycle
neuron migration
regulation of heart rate by hormone
substantia nigra development
hippocampus development
cerebral cortex development
intracellular signal transduction
regulation of membrane repolarization
membrane repolarization during cardiac muscle cell action potential
regulation of heart rate by cardiac conduction
regulation of cellular response to heat
regulation of potassium ion transmembrane transporter activity
negative regulation of peptidyl-serine dephosphorylation
regulation of signal transduction
cellular response to insulin stimulus
regulation of neuron differentiation
regulation of synaptic plasticity
small GTPase mediated signal transduction
negative regulation of ion transmembrane transport
platelet activation
negative regulation of apoptotic process
establishment of Golgi localization
Golgi reassembly
protein phosphorylation
regulation of ERK1 and ERK2 cascade
regulation of catalytic activity
histamine secretion by mast cell
protein targeting to mitochondrion
regulation of cell death
response to drug
