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14-3-3 epsilon Antibody (MA5-49207) in ICC/IF
Immunocytochemistry analysis of 14-3-3 epsilon in Caco-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in 14-3-3 epsilon Monoclonal antibody (Product # MA5-49207) using a dilution of 5 μg/mL. DyLight®594 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilutio... View More
Product Details
MA5-49207
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
3G11G2
Immunogen
E.coli-derived human YWHAE recombinant protein (Position: M1-Q255).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_3074865
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human SH-SY5Y whole cell, human SW620 whole cell, human Raji whole cell, rat brain tissue, rat PC-12 whole cell, mouse brain tissue, mouse NIH/3T3 whole cell. IHC: human colorectal adenocarcinoma tissue, human liver cancer tissue, human tonsil tissue, human endometrial cancer tissue, human placenta tissue, human bladder epithelial carcinoma tissue, human laryngeal squamous cell carcinomas tissue, rat brain tissue. ICC/IF: Caco-2 cell. Flow: ANA-1 cell, NRK cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
14-3-3 epsilon belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 14-3-3 epsilon; 14-3-3 protein epsilon; 14-3-3E; epididymis luminal protein 2; mitochondrial import stimulation factor (MSF) L subunit; Mitochondrial import stimulation factor L subunit; MSF L; protein kinase C inhibitor protein-1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activatioprotein epsilon polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activatioprotein, epsilon polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, epsilon polypeptide
Gene Aliases: 14-3-3E; AU019196; HEL2; KCIP-1; MDCR; MDS; YWHAE
UniProt ID: (Human) P62258, (Rat) P62260, (Mouse) P62259
Entrez Gene ID: (Human) 7531, (Rat) 29753, (Mouse) 22627
protein binding
potassium channel regulator activity
enzyme binding
protein domain specific binding
MHC class II protein complex binding
ubiquitin protein ligase binding
histone deacetylase binding
ion channel binding
poly(A) RNA binding
protein heterodimerization activity
phosphoserine binding
phosphoprotein binding
cadherin binding involved in cell-cell adhesion
protein kinase C inhibitor activity
protein complex binding
neuron migration
protein targeting
signal transduction
substantia nigra development
hippocampus development
cerebral cortex development
negative regulation of protein dephosphorylation
regulation of membrane repolarization
negative regulation of protein serine/threonine kinase activity
regulation of potassium ion transmembrane transporter activity
negative regulation of peptidyl-serine dephosphorylation
G2/M transition of mitotic cell cycle
regulation of heart rate by hormone
viral process
hippo signaling
intracellular signal transduction
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
membrane organization
membrane repolarization during cardiac muscle cell action potential
regulation of heart rate by cardiac conduction
cell-cell adhesion
regulation of cellular response to heat
positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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