Invitrogen

14-3-3 epsilon Recombinant Superclonal™ Antibody (5HCLC)

14-3-3 epsilon Antibody in Western Blot (WB)

图: 1 / 3

14-3-3 epsilon Antibody (711273) in WB

Western blot analysis was performed on Tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2) and Membrane extract of Jurkat (Lane 3), PC-12 (Lane 4), SW480 (Lane 5), NIH/3T3 (Lane 6), Hep G2 (Lane 7), HeLa (Lane 8) and T-47D (Lane 9). The blots were probed with Anti-14-3-3 protein epsilon/YWHAE Recombinant Rabbit Superclonal™ Antibody (Product # 711273, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500... View More

Product Image Gallery

产品信息

711273

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

产品规格

种属反应

Human, Rat

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Superclonal

类型

Antibody

克隆号

5HCLC

抗原

Peptides corresponding to Human YWHAE (aa 70-87, 238-255)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2633146

产品详细信息

This antibody is predicted to react with Monkey, Sheep, Rat and Mouse

Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

靶标信息

14-3-3 epsilon belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: 14-3-3 epsilon; 14-3-3 protein epsilon; 14-3-3E; epididymis luminal protein 2; mitochondrial import stimulation factor (MSF) L subunit; Mitochondrial import stimulation factor L subunit; MSF L; protein kinase C inhibitor protein-1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activatioprotein epsilon polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, epsilon polypeptide

查看更多收起

基因别名: 14-3-3E; HEL2; KCIP-1; MDCR; MDS; YWHAE

查看更多收起

UniProt ID: (Human) P62258, (Rat) P62260

查看更多收起

Entrez Gene ID: (Human) 7531, (Rat) 29753

查看更多收起

功能