Invitrogen

14-3-3 sigma Polyclonal Antibody

14-3-3 sigma Antibody in Immunocytochemistry (ICC/IF)

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14-3-3 sigma Antibody (PA5-81018) in ICC/IF

Immunofluorescence staining of 14-3-3 sigma in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with 14-3-3 sigma Polyclonal Antibody (Product # PA5-81018, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positiv... View More

14-3-3 sigma Antibody in Western Blot (WB)

14-3-3 sigma Antibody in Immunoprecipitation (IP)

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产品信息

PA5-81018

应用

建议稀释比

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免疫印迹 (WB)

1:500-1:2,000

免疫细胞化学 (ICC/IF)

1:300-1:10,000

酶联免疫吸附实验 (ELISA)

1:5,000-1:10,000

免疫沉淀 (IP)

1-4 µL/mg of lysate

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant Human 14-3-3 sigma/Stratifin/YWHAS Protein (Met1-Ser248)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2788273

产品详细信息

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

This antibody has specificity for Human 14-3-3 sigma/Stratifin/YWHAS.

靶标信息

This gene encodes a cell cycle checkpoint protein. The encoded protein binds to translation and initiation factors and functions as a regulator of mitotic translation. In response to DNA damage this protein plays a role in preventing DNA errors during mitosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。