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-Flow-20170216130641.jpg?time=20240412 "CD305 (LAIR1) Antibody in Flow Cytometry (Flow)")
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CD305 (LAIR1) Antibody (14-3051-82) in Flow
Staining of C57BL/6 splenocytes with 0.125 µg of Armenian Hamster IgG Isotype Control Purified (Product # 14-4888-81) (purple line) or 0.125 µg of Anti-Mouse CD305 (LAIR-1) Purified (blue line) followed by Anti-Armenian Hamster IgG PE (Product # 12-4112-83). Total viable F4/80+ cells were used for analysis.
-Flow-20170216130641.jpg?time=20240412)
-Flow-20170216130641.jpg?time=20240412?time=20240412 "CD305 (LAIR1) Antibody (14-3051-82) in Flow")
产品信息
14-3051-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Mouse
宿主/亚型
Armenian hamster
/ IgG
分类
Monoclonal
类型
Antibody
克隆号
113
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_1210499
产品详细信息
Description: CD305 or Leukocyte-associated Ig-like receptor-1 (LAIR-1) is a 46 kD cell surface molecule that functions as an inhibitory collagen-receptor on the majority of cells of the immune system. The mouse LAIR-1 molecule is expressed primarily on monocytes/macrophages and also on T cells, NK cells and dendritic cells. Unlike human B cells , mouse B cells do not express LAIR-1. The cytoplasmic tail of mouse LAIR-1 can be phosphorylated leading to the recruitment of SH2-containing tyrosine phosphatase(SHP)-2 and C-terminal Src kinase.
Applications Reported: This 113 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunoblotting (WB).
Applications Tested: This 113 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
LAIR1 (CD305) functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation. May also play its inhibitory role independently of SH2-containing phosphatases. Modulates cytokine production in CD4+ T-cells, down-regulating IL2 and IFNG production while inducing secretion of transforming growth factor beta. Down-regulates also IgG and IgE production in B-cells as well as IL8, IL10 and TNF secretion. Inhibits proliferation and induces apoptosis in myeloid leukemia cell lines as well as prevents nuclear translocation of NF-kappa-B p65 subunit/RELA and phosphorylation of I-kappa-B alpha/CHUK in these cells. Inhibits the differentiation of peripheral blood precursors towards dendritic cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CD305; LAIR-1; Leukocyte-associated immunoglobulin-like receptor 1
基因别名: 5133400O11Rik; BB115266; D7Bwg0421e; Lair-1; Lair1
UniProt ID: (Mouse) Q8C6N8
Entrez Gene ID: (Mouse) 52855
