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CD8b Antibody (14-5273-82) in Flow
(Left) Staining of normal human peripheral blood cells either with Anti-Human CD8a (Product # 12-0086) and Anti-Human/Non-Human Primate CD8b Purified. F(ab')2 Anti-Mouse IgG PE (Product # 12-4012) was used to detect Anti-CD8b Purified. Cells in the lymphocyte gate were used for analysis. (Right) Immunoblotting of reduced cell lysate prepared from normal human peripheral blood mononuclear cells with 5 µg/mL of Anti-Human/Non-Human Primate CD8b antibody. Bands were visualized using Anti-Mouse IgG HRP.
产品信息
14-5273-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Non-human primate
已发表种属
Human,
Rhesus monkey
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
SIDI8BEE
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_11040011
产品详细信息
Description: The SIDI8BEE monoclonal antibody reacts with human and rhesus macaque CD8 beta. The CD8b chain associates with the CD8a chain to form the CD8ab heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alpha beta TCR T cells. The CD8 receptor complex binds to MHC class I and plays a role in T cell development and activation of mature T cells. Long been thought to exist either as a heterodimer of the alpha and beta chain or as a homodimer of alpha chains, beta chains have now been shown to homodimerize as well. SIDI8BEE can detect the CD8 beta chain in both the CD8ab heterodimer and the CD8bb homodimer.
SIDI8BEE is cross-reactive to human and rhesus macaque CD8b. Other non-human primate species have not been tested.
Applications Reported: This SIDI8BEE antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and western blotting.
Applications Tested: This SIDI8BEE antibody has been tested by flow cytometric analysis of human peripheral blood lymphocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
The CD8B antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CD8; CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8alpha; CD8b; CD8beta; Leu-2; MAL; p32; T lymphocyte surface glycoprotein beta chain; T-cell surface glycoprotein CD8 beta chain
基因别名: CD8B; CD8B1; LEU2; LY3; LYT3; P37
UniProt ID: (Human) P10966
Entrez Gene ID: (Human) 926
