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Oligodendrocyte Marker O1 Antibody (14-6506-82) in ICC/IF
Immunocytochemistry of fixed, differentiated OLN93 cells using 10 µg/mL of Mouse IgM Isotype Control Purified (Product # 14-4752-82) (left) or 10 µg/mL Anti-Oligodendrocyte Marker O1 (right) followed by Anti-Mouse IgM FITC (Product # 11-5790-81). Nuclei are stained with DAPI.
产品信息
14-6506-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Chicken,
Human,
Mouse,
Rat
已发表种属
Human,
Mouse
宿主/亚型
Mouse
/ IgM
分类
Monoclonal
类型
Antibody
克隆号
O1
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_10718397
产品详细信息
Description: This O1 monoclonal antibody reacts with galactocerebroside and other lipids found on late oligodendrocyte precursors. O1 has been reported to react to all species tested (rat, mouse, human, chicken). Oligodendrocytes function as myelin-producing cells within the central nervous system ensheathing the axons of neurons with myelin in order to facilitate rapid axonal conduction. O1 and O4 monoclonal antibodies serve as stage-specific markers of oligodendrocyte development with O4 marking early oligodendrocyte progenitors and O1-labeling late oligodendrocyte progenitors. O4-positive cells differentiate to become O1-positive, which can in turn become mature oligodendrocytes.
Applications Reported: This O1 antibody has been reported for use in flow cytometric analysis, immunohistochemical staining of frozen tissue sections, ELISA, and immunocytochemistry.
Applications Tested: This O1 antibody has been tested by immunocytochemistry on formaldehyde-fixed, differentiated OLN93 cells and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Oligodendrocytes function as myelin-producing cells within the central nervous system ensheathing the axons of neurons with myelin in order to facilitate rapid axonal conduction. O1 and O4 monoclonal antibodies serve as stage-specific markers of oligodendrocyte development with O4 marking early oligodendrocyte progenitors and O1-labeling late oligodendrocyte progenitors. O4-positive cells differentiate to become O1-positive, which can in turn become mature oligodendrocytes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: O1 Marker
