Invitrogen

IFN gamma Monoclonal Antibody (AN-18), eBioscience™

IFN gamma Antibody in Immunostaining (IS)

图: 1 / 3

IFN gamma Antibody (14-7313-81) in IS

Splenocytes from mice pre-immunized with PLP139-151 peptide were recall activated 4 weeks later with PLP139-151 for 24 hours in Mouse IFN gamma ELISPOT assay. Control well is medium alone.

IFN gamma Antibody in T-Cell Activation (TCA)

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产品信息

14-7313-81

应用

建议稀释比

已发表文章

酶联免疫吸附实验 (ELISA)

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免疫沉淀 (IP)

Assay-Dependent

功能检测 (Functional)

查看2篇已发表文章 2篇已发表文章

放射免疫测定 (RIA)

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T细胞活化 (TCA)

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免疫染色 (IS)

0.25-1 µg/mL

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

AN-18

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_468471

产品详细信息

Description: The AN-18 antibody reacts with mouse interferon-gamma (IFN gamma), a 15-20 kDa cytokine secreted by Th1 cells.

Applications Reported: The AN-18 antibody has been reported for use in immunoprecipitation, ELISPOT, and ELISA. (Please use Functional Grade purified AN-18, Product # 16-7313, for ELISPOT Capture).

Applications Tested: The AN-18 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse IFNg (Interferon-gamma, IFN-g) in combination with the biotin R4-6A2 (13-7312) antibody for detection and recombinant mouse IFNg (39-8311) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 0.25-1 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 200 pg/mL - 1.6 pg/mL should be included in each ELISA plate.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

An ex vivo model of Toxoplasma recrudescence reveals developmental plasticity of the bradyzoite stage.

mBio

Vizcarra EA,Goerner AL,Ulu A,Hong DD,Bergersen KV,Talavera MA,Le Roch K,Wilson EH,White MW

14-7313-81 was used in ELISA to investigate the molecular basis of growth and cell-dependent reactivation pathways, single-cell mRNA sequencing was performed on recrudescing parasites, revealing distinct gene signatures of these parasite populations and confirming multifunctionality of the original ex vivo bradyzoite population.

Tue Oct 31 00:00:00 UTC 2023

Novel HLA-B7-restricted human metapneumovirus epitopes enhance viral clearance in mice and are recognized by human CD8⁺ T cells.

Scientific reports

Miranda-Katz M,Erickson JJ,Lan J,Ecker A,Zhang Y,Joyce S,Williams JV

14-7313-81 was used in ELISA to enable identification of HMPV-specific CD8+ T cells in humans and help to inform future HMPV vaccine design.

Thu Oct 21 00:00:00 UTC 2021

Novel HLA-B7-restricted human metapneumovirus epitopes enhance viral clearance in mice and are recognized by human CD8⁺ T cells.

Scientific reports

Miranda-Katz M,Erickson JJ,Lan J,Ecker A,Zhang Y,Joyce S,Williams JV

14-7313-81 was used in ELISA to enable identification of HMPV-specific CD8+ T cells in humans and help to inform future HMPV vaccine design.

Thu Oct 21 00:00:00 UTC 2021

T-B Lymphocyte Interactions Promote Type 1 Diabetes Independently of SLAM-Associated Protein.

Journal of immunology (Baltimore, Md. : 1950)

Bonami RH,Nyhoff LE,McNitt DH,Hulbert C,Felton JL,Kendall PL,Thomas JW

14-7313-81 was used in ELISA to understand how fully developed T follicular helper cells contribute to different autoimmune processes, investigating the role of signaling lymphocytic activation molecule-associated protein in type 1 diabetes and autoantibody-mediated arthritis.

Tue Dec 15 00:00:00 UTC 2020

Neurokinin-1 Receptor Signaling Is Required for Efficient Ca²⁺ Flux in T-Cell-Receptor-Activated T Cells.

Cell reports

Morelli AE,Sumpter TL,Rojas-Canales DM,Bandyopadhyay M,Chen Z,Tkacheva O,Shufesky WJ,Wallace CT,Watkins SC,Berger A,Paige CJ,Falo LD,Larregina AT

Published figure using IFN gamma monoclonal antibody (Product # 14-7313-81) in T-Cell Activation

Tue Mar 10 00:00:00 UTC 2020

Rab6-dependent retrograde traffic of LAT controls immune synapse formation and T cell activation.

The Journal of experimental medicine

Carpier JM,Zucchetti AE,Bataille L,Dogniaux S,Shafaq-Zadah M,Bardin S,Lucchino M,Maurin M,Joannas LD,Magalhaes JG,Johannes L,Galli T,Goud B,Hivroz C

14-7313 was used in ELISA to study the retrograde traffic of linker for activation of T cells from the plasma membrane to the Golgi-trans-Golgi network.

Mon Apr 02 00:00:00 UTC 2018

IFNγ PET Imaging as a Predictive Tool for Monitoring Response to Tumor Immunotherapy.

Cancer research

Gibson HM,McKnight BN,Malysa A,Dyson G,Wiesend WN,McCarthy CE,Reyes J,Wei WZ,Viola-Villegas NT

14-7313-81 was used in Radioimmune Assays to present a novel approach to monitor therapeutic outcomes via IFNγ-targeted positron emission tomography.

Mon Oct 01 00:00:00 UTC 2018

Serum Neuroinflammatory Disease-Induced Central Nervous System Proteins Predict Clinical Onset of Experimental Autoimmune Encephalomyelitis.

Frontiers in immunology

Raphael I,Webb J,Gomez-Rivera F,Chase Huizar CA,Gupta R,Arulanandam BP,Wang Y,Haskins WE,Forsthuber TG

14-7313 was used in an ELISpot assay to determine if changes in serum neuroinflammatory disease-induced CNS proteins are detectable prior to clinical onset of experimental autoimmune encephalomyelitis and, therefore, enable prediction of disease onset.

Wed Nov 20 00:00:00 UTC 2019

Double conjugation strategy to incorporate lipid adjuvants into multiantigenic vaccines.

Chemical science

Hussein WM,Liu TY,Maruthayanar P,Mukaida S,Moyle PM,Wells JW,Toth I,Skwarczynski M

14-7313 was used in an ELISpot assay to develop a double conjugation strategy for the synthesis of self-adjuvanting, branched, multiantigenic vaccine candidates.

Tue Mar 01 00:00:00 UTC 2016

Nonclassical MHC Ib-restricted CD8+ T Cells Recognize Mycobacterium tuberculosis-Derived Protein Antigens and Contribute to Protection Against Infection.

PLoS pathogens

Shang S,Siddiqui S,Bian Y,Zhao J,Wang CR

14-7313 was used in an ELISpot assay to investigate the relative contribution of various MHC Ib-restricted T cell populations to anti-mycobacterial immunity.

Wed Jun 01 00:00:00 UTC 2016

Altered Glycosylation Patterns Increase Immunogenicity of a Subunit Hepatitis C Virus Vaccine, Inducing Neutralizing Antibodies Which Confer Protection in Mice.

Journal of virology

Li D,von Schaewen M,Wang X,Tao W,Zhang Y,Li L,Heller B,Hrebikova G,Deng Q,Ploss A,Zhong J,Huang Z

14-7313 was used in an ELISpot assay to identify a promising broadly protective HCV vaccine candidate that should be considered for further preclinical and clinical development.

Thu Dec 01 00:00:00 UTC 2016

Mucosal Langerhans Cells Promote Differentiation of Th17 Cells in a Murine Model of Periodontitis but Are Not Required for Porphyromonas gingivalis-Driven Alveolar Bone Destruction.

Journal of immunology (Baltimore, Md. : 1950)

Bittner-Eddy PD,Fischer LA,Kaplan DH,Thieu K,Costalonga M

14-7313 was used in an ELISpot assay to indicate that both Langerhans cells and Th17 cells are redundant in contributing to alveolar bone destruction in a murine model of periodontitis.

Mon Aug 15 00:00:00 UTC 2016

Targeted Vaccination against Human α-Lactalbumin for Immunotherapy and Primary Immunoprevention of Triple Negative Breast Cancer.

Cancers

Tuohy VK,Jaini R,Johnson JM,Loya MG,Wilk D,Downs-Kelly E,Mazumder S

14-7313 was used in an ELISpot assay to investigate the use of retired self-proteins in the treatment of cancer, showing that vaccination against α-Lactalbumin can be used against triple negative breast cancers.

Thu Jun 16 00:00:00 UTC 2016

Macrophage migration inhibitory factor promotes resistance to glucocorticoid treatment in EAE.

Neurology(R) neuroimmunology & neuroinflammation

Ji N,Kovalovsky A,Fingerle-Rowson G,Guentzel MN,Forsthuber TG

14-7313 was used in an ELISpot assay to establish macrophage migration inhibitory factor as a key molecule underlying resistance to anti-inflammatory treatment for multiple sclerosis.

Thu Oct 01 00:00:00 UTC 2015

Blocking stroke-induced immunodeficiency increases CNS antigen-specific autoreactivity but does not worsen functional outcome after experimental stroke.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Römer C,Engel O,Winek K,Hochmeister S,Zhang T,Royl G,Klehmet J,Dirnagl U,Meisel C,Meisel A

Wed May 20 00:00:00 UTC 2015

Natural killer T cells are essential for the development of contact hypersensitivity in BALB/c mice.

The Journal of investigative dermatology

Shimizuhira C,Otsuka A,Honda T,Kitoh A,Egawa G,Nakajima S,Nakashima C,Watarai H,Miyachi Y,Kabashima K

14-7313 was used in Flow cytometry/Cell sorting to report the pivotal contribution of dendritic and natural killer T cell interactions to hypersensitivity.

Sat Nov 01 00:00:00 UTC 2014

The aryl hydrocarbon receptor promotes IL-10 production by NK cells.

Journal of immunology (Baltimore, Md. : 1950)

Wagage S,John B,Krock BL,Hall AO,Randall LM,Karp CL,Simon MC,Hunter CA

14-7313 was used in an ELISA assay to investigate the source of IL-10 during Toxoplasma gondii infection.

Sat Feb 15 00:00:00 UTC 2014

Vaccination with a single CD4 T cell peptide epitope from a Salmonella type III-secreted effector protein provides protection against lethal infection.

Infection and immunity

Kurtz JR,Petersen HE,Frederick DR,Morici LA,McLachlan JB

14-7313 was used in an ELISpot assay to investigate the immune response elicited against Salmonella to gather information for future vaccine design.

Sun Jun 01 00:00:00 UTC 2014

Conventional housing conditions attenuate the development of experimental autoimmune encephalomyelitis.

PloS one

Arndt A,Hoffacker P,Zellmer K,Goecer O,Recks MS,Kuerten S

14-7313 was used in an ELISpot assay to investigate the effect of pathogens on EAE, showing that conventional housing conditions attenuate the development of EAE.

Wed Sep 30 00:00:00 UTC 2015

Regulation of adaptive immunity by the fractalkine receptor during autoimmune inflammation.

Journal of immunology (Baltimore, Md. : 1950)

Garcia JA,Pino PA,Mizutani M,Cardona SM,Charo IF,Ransohoff RM,Forsthuber TG,Cardona AE

14-7313 was used in an ELISpot assay to determine the role of CX3CR1 in the trafficking and function of myeloid cells during experimental autoimmune encephalomyelitis.

Thu Aug 01 00:00:00 UTC 2013

B lymphocyte "original sin" in the bone marrow enhances islet autoreactivity in type 1 diabetes-prone nonobese diabetic mice.

Journal of immunology (Baltimore, Md. : 1950)

Henry-Bonami RA,Williams JM,Rachakonda AB,Karamali M,Kendall PL,Thomas JW

14-7313 was used in Flow cytometry/Cell sorting to better understand the development of anti-insulin B cells by tracking their development in the polyclonal repertoire of VH125Tg mice.

Sat Jun 15 00:00:00 UTC 2013

Small molecule inhibitor of antigen binding and presentation by HLA-DR2b as a therapeutic strategy for the treatment of multiple sclerosis.

Journal of immunology (Baltimore, Md. : 1950)

Ji N,Somanaboeina A,Dixit A,Kawamura K,Hayward NJ,Self C,Olson GL,Forsthuber TG

14-7313 was used in an ELISpot assay to investigate how blocking the HLA-DR2b allele with small molecule inhibitors may offer a promising therapeutic strategy for the treatment of multiple sclerosis.

Fri Nov 15 00:00:00 UTC 2013

A T cell epitope-based vaccine protects against chlamydial infection in HLA-DR4 transgenic mice.

Vaccine

Li W,Murthy AK,Lanka GK,Chetty SL,Yu JJ,Chambers JP,Zhong G,Forsthuber TG,Guentzel MN,Arulanandam BP

14-7313 was used in an ELISpot assay to investigate CD4+ T cell reactive chlamydial protease-like activity factor epitopes and characterise activation of T cells following antigen immunisation.

Tue Nov 19 00:00:00 UTC 2013

MHC mismatch results in neural progenitor cell rejection following spinal cord transplantation in a model of viral-induced demyelination.

Stem cells (Dayton, Ohio)

Weinger JG,Weist BM,Plaisted WC,Klaus SM,Walsh CM,Lane TE

14-7313 was used in an ELISpot assay to investigate whether allogeneic neural progenitor cells are tolerated or induce immune-mediated rejection under neuroinflammatory demyelinating conditions.

Thu Nov 01 00:00:00 UTC 2012

Dietary n-3 polyunsaturated fatty acids (PUFA) decrease obesity-associated Th17 cell-mediated inflammation during colitis.

PloS one

Monk JM,Hou TY,Turk HF,Weeks B,Wu C,McMurray DN,Chapkin RS

14-7313 was used in Functional assays to monitor changes in immune cell subsets and inflammatory biomarker expression in a mouse model of concurrent obesity and colitis.

Thu Jun 20 00:00:00 UTC 2013

Virus-induced tumor inflammation facilitates effective DC cancer immunotherapy in a Treg-dependent manner in mice.

The Journal of clinical investigation

Woller N,Knocke S,Mundt B,Gürlevik E,Strüver N,Kloos A,Boozari B,Schache P,Manns MP,Malek NP,Sparwasser T,Zender L,Wirth TC,Kubicka S,Kühnel F

14-7313 was used in an ELISpot assay to highlight viruses as attractive tools for eliciting effective antitumour responses upon DC vaccination.

Fri Jul 01 00:00:00 UTC 2011

Inhibition of SOCS1-/- lethal autoinflammatory disease correlated to enhanced peripheral Foxp3+ regulatory T cell homeostasis.

Journal of immunology (Baltimore, Md. : 1950)

Collins EL,Jager LD,Dabelic R,Benitez P,Holdstein K,Lau K,Haider MI,Johnson HM,Larkin J

Published figure using IFN gamma monoclonal antibody (Product # 14-7313-81) in ELISA

Thu Sep 01 00:00:00 UTC 2011

Proteasomes, TAP, and endoplasmic reticulum-associated aminopeptidase associated with antigen processing control CD4+ Th cell responses by regulating indirect presentation of MHC class II-restricted cytoplasmic antigens.

Journal of immunology (Baltimore, Md. : 1950)

Dragovic SM,Hill T,Christianson GJ,Kim S,Elliott T,Scott D,Roopenian DC,Van Kaer L,Joyce S

14-7313 was used in an ELISpot assay to study a novel model in which the cytosolic Ag-processing machinery regulates the quantity of cytoplasmic peptides available for presentation by class II molecules and, hence, modulates Th cell responses.

Wed Jun 15 00:00:00 UTC 2011

In vivo requirement for Atg5 in antigen presentation by dendritic cells.

Immunity

Lee HK,Mattei LM,Steinberg BE,Alberts P,Lee YH,Chervonsky A,Mizushima N,Grinstein S,Iwasaki A

14-7313 was used in an ELISpot assay to investigate how dendritic cells utilise autophagic machinery to optimally present extracellular microbial antigens for MHC II molecules.

Fri Feb 26 00:00:00 UTC 2010

CD137 agonist antibody prevents cancer recurrence: contribution of CD137 on both hematopoietic and nonhematopoietic cells.

Blood

Narazaki H,Zhu Y,Luo L,Zhu G,Chen L

14-7313 was used in an ELISpot assay to investigate methods to induce memory T cells to prevent tumour recurrence.

Thu Mar 11 00:00:00 UTC 2010

Selectivity of oncolytic viral replication prevents antiviral immune response and toxicity, but does not improve antitumoral immunity.

Molecular therapy : the journal of the American Society of Gene Therapy

Gürlevik E,Woller N,Strüver N,Schache P,Kloos A,Manns MP,Zender L,Kühnel F,Kubicka S

14-7313 was used in an ELISpot assay to investigate how the degree of tumor selectivity of viral replication marginally influences antitumoral immune responses, but is a major determinant of antivector immunity and systemic toxicity.

Mon Nov 01 00:00:00 UTC 2010

CD4+ T cells elicit host immune responses to MHC class II-negative ovarian cancer through CCL5 secretion and CD40-mediated licensing of dendritic cells.

Journal of immunology (Baltimore, Md. : 1950)

Nesbeth YC,Martinez DG,Toraya S,Scarlett UK,Cubillos-Ruiz JR,Rutkowski MR,Conejo-Garcia JR

14-7313 was used in an ELISpot assay to investigate how to incorporate tumor-reactive CD4(+) T cells in adoptive cell transfer immunotherapies against ovarian cancer.

Sat May 15 00:00:00 UTC 2010

CCR7-dependent immunity during acute Toxoplasma gondii infection.

Infection and immunity

Noor S,Habashy AS,Nance JP,Clark RT,Nemati K,Carson MJ,Wilson EH

14-7313 was used in an ELISA assay to investigate the role of CCR7 signaling in generating protective immune responses to the intracellular protozoan parasite Toxoplasma gondii.

Sat May 01 00:00:00 UTC 2010

MHC class II-dependent basophil-CD4+ T cell interactions promote T(H)2 cytokine-dependent immunity.

Nature immunology

Perrigoue JG,Saenz SA,Siracusa MC,Allenspach EJ,Taylor BC,Giacomin PR,Nair MG,Du Y,Zaph C,van Rooijen N,Comeau MR,Pearce EJ,Laufer TM,Artis D

14-7313 was used in an ELISA assay to suggest that major histocompatibility complex class II-dependent interactions between basophils and CD4+ T cells promote T helper type 2 cytokine responses and immunity to helminth infection.

Wed Jul 01 00:00:00 UTC 2009

Human bone marrow-derived mesenchymal stem cells induce Th2-polarized immune response and promote endogenous repair in animal models of multiple sclerosis.

Glia

Bai L,Lennon DP,Eaton V,Maier K,Caplan AI,Miller SD,Miller RH

14-7313 was used in an ELISpot assay to suggest that bone marrow-derived mesenchymal stem cells represent a viable option for therapeutic approaches to multiple sclerosis.

Sat Aug 15 00:00:00 UTC 2009

Encephalitogenicity of complete Freund's adjuvant relative to CpG is linked to induction of Th17 cells.

Journal of immunology (Baltimore, Md. : 1950)

Tigno-Aranjuez JT,Jaini R,Tuohy VK,Lehmann PV,Tary-Lehmann M

14-7313 was used in an ELISPOT assay to compare both complete Freund's adjuvant-induced, and CpG induced models of experimental autoimmune encephalmyelitis, showing that CpG results in fewer Ag-specific IL-17 cells.

Sun Nov 01 00:00:00 UTC 2009

Estradiol-induced vaginal mucus inhibits antigen penetration and CD8(+) T cell priming in response to intravaginal immunization.

Vaccine

Seavey MM,Mosmann TR

14-7313 was used in an ELISpot assay to investigate the effect of estradiol during intravaginal immunisation, showing that estradiol-induced vaginal mucus inhibits antigen penetration and CD8(+) T cell priming.

Tue Apr 14 00:00:00 UTC 2009

IL-10 from CD4CD25Foxp3CD127 adaptive regulatory T cells modulates parasite clearance and pathology during malaria infection.

PLoS pathogens

Couper KN,Blount DG,Wilson MS,Hafalla JC,Belkaid Y,Kamanaka M,Flavell RA,de Souza JB,Riley EM

14-7313 was used in an ELISA assay to investigate the cellular source of IL-10 and TGF-beta during malaria infection, showing that IL-10 from CD4+CD25-Foxp3-CD127- adaptive Tregs modulates parasite clearance and pathology.

Fri Feb 29 00:00:00 UTC 2008

Macrophage-mediated but gamma interferon-independent innate immune responses control the primary wave of Plasmodium yoelii parasitemia.

Infection and immunity

Couper KN,Blount DG,Hafalla JC,van Rooijen N,de Souza JB,Riley EM

14-7313 was used in an ELISA assay to discern the factors that control the primary wave of plasmodium yoelii parasitaemia.

Sat Dec 01 00:00:00 UTC 2007

Efficient presentation of myelin oligodendrocyte glycoprotein peptides but not protein by astrocytes from HLA-DR2 and HLA-DR4 transgenic mice.

Journal of neuroimmunology

Kort JJ,Kawamura K,Fugger L,Weissert R,Forsthuber TG

Sat Apr 01 00:00:00 UTC 2006

生物信息学

蛋白别名: gamma interferon; H-IFN-g; IFN γ; IFN-gamma; IFN-y; IFNγ; Interferon; Interferon gamma; Interferon y; Interferon γ; Interferonγ; M-IFN-g; R-IFN-g

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基因别名: Ifg; IFN-g; Ifng

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UniProt ID: (Mouse) P01580

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Entrez Gene ID: (Mouse) 15978

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功能

cytokine activity interferon-gamma receptor binding protein binding cytokine interferon superfamily intercellular signal molecule

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