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Cytokeratin 7 Antibody (14-9005-82) in ICC/IF
Immunofluorescence analysis of Cytokeratin 7 was performed using HeLa and SK-OV-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Cytokeratin 7 Monoclonal Antibody (LP5K), eBioscience™ (Product # 14-9005-82) at 5 microgram/mL in 0.1% BSA, incubated at... View More
产品信息
14-9005-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human,
Mouse
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
LP5K
偶联物
Unconjugated
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_10669584
产品详细信息
Description: This LP5K monoclonal antibody reacts with human cytokeratin 7 (K7), a 54-kDa type II (or basic) keratin expressed either alone or paired with cytokeratin 19 in simple epithelia, mesothelium, urothelium, and pseudostratified epithelium. Expression of cytokeratin 7 in the gastric foveolar, intestinal, and stratified squamous epithelia is extremely low or undetectable. Cytokeratins form the intracellular cytoskeletal network that maintains the integrity and stability of cells and tissues. In addition, most carcinomas express cytokeratin 7. The coordinated expression of this keratin with cytokeratin 20 is commonly used as a diagnostic marker for a variety of carcinomas.
Applications Reported: This LP5K antibody has been reported for use in immunoblotting (WB) and immunocytochemistry (ICC).
Applications Tested: This LP5K antibody has been tested by immunofluorescent staining of paraformaldehyde fixed and permeabilized cells. This can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Cytokeratin 7 blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CK-7; cytokeratin 7; Cytokeratin-7; K7; keratin 7, type II; keratin, 55K type II cytoskeletal; keratin, simple epithelial type I, K7; Keratin, type II cytoskeletal 7; Keratin-7; Sarcolectin; type II mesothelial keratin K7; Type-II keratin Kb7
基因别名: CK7; K2C7; K7; KRT7; SCL
UniProt ID: (Human) P08729
Entrez Gene ID: (Human) 3855
