Invitrogen

PCDH1 (Protocadherin 1) Monoclonal Antibody (CSTEM31), eBioscience™

PCDH1 (Protocadherin 1) Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 2

PCDH1 (Protocadherin 1) Antibody (14-9157-82) in ICC/IF

Immunocytochemistry of fixed and permeabilized human iPSCs plated on murine derived feeder cells using 5 µg/mL Mouse IgG2b K Isotype Control (Product # 14-4732-82) (left) or 5 µg/mL Anti-Human PCDH1 (Protocadherin 1) purified (right) followed by F(ab’)2-Goat anti-Mouse IgG Secondary Antibody eFluor® 660 (Product # 50-4010-82). Nuclei are stained with DAPI.

PCDH1 (Protocadherin 1) Antibody in Flow Cytometry (Flow)

Product Image Gallery

产品信息

14-9157-82

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

5 µg/mL

流式细胞分析 (Flow)

0.5 µg/test

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

CSTEM31

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with proprietary stabilizer

内含物

0.09% sodium azide

保存条件

4° C, do not freeze

运输条件

Wet ice

AB_2716937

产品详细信息

This monoclonal antibody CSTEM31 reacts with human Protocadherin-1, also known as PCDH1 and cadherin-like protein 1.

Applications Reported: This CSTEM31 antibody has been reported for use in immunocytochemical staining and flow cytometric analysis. (Fluorochrome-conjugated CSTEM31 is recommended for use in flow cytometery.)

Applications Tested: This CSTEM31 antibody has been tested by immunocytochemistry on formaldehyde-fixed and permeabilized human iPSCs, and by flow cytometry on human iPSCs. For immunocytochemistry, this can be used at less than or equal to 5 µg/mL. For flow cytometry, this antibody can be used at less than or equal to 0.5 µg/test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

靶标信息

Protocadherin 1, also known as PCDH1 and cadherin-like protein 1, is a transmembrane protein involved in cell-cell adhesion. Protocadherin 1 belongs to a group of cadherin-like proteins termed delta1-protocadherins, which consists of protocadherin-1, -7, -9, and -11(X/Y). The delta1-protocadherins have seven extracellular cadherin-repeats, a transmembrane domain, and a cytoplasmic domain, and can be expressed in two isoforms, short or long. Expression of protocadherin 1 is regulated both temporally and spatially, and plays a role both in early development and mature tissues. In mice, it is expressed in a number of tissues and organs, including the brain, spinal cord, peripheral ganglia, inner ear, hair follicles, kidney, reproductive organs, prostate, and the seminal gland. Notably, protocadherin 1 is a susceptibility gene for Bronchial Hyperresponsiveness in humans. Expression of protocadherin 1 has also been identified on human pluripotent stem cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。