Invitrogen

TCR V alpha 2 Monoclonal Antibody (B20.1), APC, eBioscience™

TCR V alpha 2 Antibody in Flow Cytometry (Flow)

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TCR V alpha 2 Antibody (17-5812-82) in Flow

Staining of C57BL/6 splenocytes with Anti-Mouse CD4 FITC (Product # 11-0042-82), Anti-Mouse CD8a FITC (Product # 11-0081-82) and 0.25 µg of Rat IgG2a K Isotype Control APC (Product # 17-4321-81) (left) or 0.25 µg of Anti-Mouse V alpha 2 TCR APC (right). Total viable cells were used for analysis.

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产品信息

17-5812-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.5 µg/test

查看13篇已发表文章 13篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a, lambda

分类

Monoclonal

类型

Antibody

克隆号

B20.1

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_1659733

产品详细信息

Description: The B20.1 monoclonal antibody reacts with mouse T-cell receptor (TCR) V alpha 2 chains. This antibody recognizes the majority of the TCR V alpha 2 subfamily in mice carrying the a, b and c haplotypes. The antibody also reacts with the products of T cell receptor, V delta 8 due to the high degree of homology.

Applications Reported: This B20.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This B20.1 antibody has been tested by flow cytometric analysis of mouse lymph node cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.It is recommended to preblock Fc receptors for 10 minutes using 0.5 µg of purified anti-mouse CD16/32 (Product # 14-0161-85). It is also recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease. Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

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Product References

Ageing promotes early T follicular helper cell differentiation by modulating expression of RBPJ.

Aging cell

Webb LMC,Fra-Bido S,Innocentin S,Matheson LS,Attaf N,Bignon A,Novarino J,Fazilleau N,Linterman MA

17-5812-82 was used in Flow Cytometry to show increased formation of Tfh precursors (pre-Tfh) but no associated increase in germinal centre (GC)-Tfh cells in aged mice, suggesting age-driven promotion of only early Tfh cell differentiation.

Fri Jan 01 00:00:00 UTC 2021

PTPN2 Deficiency Enhances Programmed T Cell Expansion and Survival Capacity of Activated T Cells.

Cell reports

Flosbach M,Oberle SG,Scherer S,Zecha J,von Hoesslin M,Wiede F,Chennupati V,Cullen JG,List M,Pauling JK,Baumbach J,Kuster B,Tiganis T,Zehn D

17-5812 was used in Flow cytometry/Cell sorting to show the therapeutic potential of targeting PTPN2 in T cell-based therapies to augment the number and survival capacity of antigen-specific T cells.

Tue Jul 28 00:00:00 UTC 2020

Attenuation of TCR-induced transcription by Bach2 controls regulatory T cell differentiation and homeostasis.

Nature communications

Sidwell T,Liao Y,Garnham AL,Vasanthakumar A,Gloury R,Blume J,Teh PP,Chisanga D,Thelemann C,de Labastida Rivera F,Engwerda CR,Corcoran L,Kometani K,Kurosaki T,Smyth GK,Shi W,Kallies A

17-5812 was used in Flow cytometry/Cell sorting to show homeostasis of thymically-derived and peripherally-derived Treg cells is maintained through the balancing of TCR signaling induced transcriptional activity by Bach2.

Tue Jan 14 00:00:00 UTC 2020

Tolerogenic Nanoparticles Induce Antigen-Specific Regulatory T Cells and Provide Therapeutic Efficacy and Transferrable Tolerance against Experimental Autoimmune Encephalomyelitis.

Frontiers in immunology

LaMothe RA,Kolte PN,Vo T,Ferrari JD,Gelsinger TC,Wong J,Chan VT,Ahmed S,Srinivasan A,Deitemeyer P,Maldonado RA,Kishimoto TK

17-5812-82 was used in Flow cytometry/Cell sorting to further show that tolerogenic nanoparticles can trigger the expansion of endogenous Tregs specific to a target Ag.

Thu Mar 21 00:00:00 UTC 2019

Langerin⁺ CD8α⁺ Dendritic Cells Drive Early CD8⁺ T Cell Activation and IL-12 Production During Systemic Bacterial Infection.

Frontiers in immunology

Prendergast KA,Daniels NJ,Petersen TR,Hermans IF,Kirman JR

17-5812 was used in Flow cytometry/Cell sorting to investigate the impact of depleting langerin+ CD8α+ dendritic cells in a murine model of intravenous infection with Mycobacterium bovis bacille Calmette-Guerin.

Mon Jul 01 00:00:00 UTC 2019

Erythropoietin Receptor-Mediated Molecular Crosstalk Promotes T Cell Immunoregulation and Transplant Survival.

Journal of the American Society of Nephrology : JASN

Purroy C,Fairchild RL,Tanaka T,Baldwin WM,Manrique J,Madsen JC,Colvin RB,Alessandrini A,Blazar BR,Fribourg M,Donadei C,Maggiore U,Heeger PS,Cravedi P

17-5812 was used in Flow cytometry/Cell sorting to delineate the protolerogenic properties of EPO in inhibiting conventional T cells while simultaneously promoting Treg induction.

Tue Aug 01 00:00:00 UTC 2017

Macrophages transfer antigens to dendritic cells by releasing exosomes containing dead-cell-associated antigens partially through a ceramide-dependent pathway to enhance CD4(+) T-cell responses.

Immunology

Xu Y,Liu Y,Yang C,Kang L,Wang M,Hu J,He H,Song W,Tang H

Published figure using TCR V alpha 2 monoclonal antibody (Product # 17-5812-82) in Flow Cytometry

Sat Oct 01 00:00:00 UTC 2016

Normal T cell selection occurs in CD205-deficient thymic microenvironments.

PloS one

Jenkinson WE,Nakamura K,White AJ,Jenkinson EJ,Anderson G

Published figure using TCR V alpha 2 monoclonal antibody (Product # 17-5812-82) in Flow Cytometry

Mon Jun 24 00:00:00 UTC 2013

Bile retinoids imprint intestinal CD103+ dendritic cells with the ability to generate gut-tropic T cells.

Mucosal immunology

Jaensson-Gyllenbäck E,Kotarsky K,Zapata F,Persson EK,Gundersen TE,Blomhoff R,Agace WW

17-5812 was used in Flow cytometry/Cell sorting to demonstrate that imprinting of CD103(+) DCs is itself critically dependent on vitamin A and occurs locally within the small intestine (SI).

Fri Jul 01 00:00:00 UTC 2011

ITAM signaling in dendritic cells controls T helper cell priming by regulating MHC class II recycling.

Blood

Graham DB,Akilesh HM,Gmyrek GB,Piccio L,Gilfillan S,Sim J,Belizaire R,Carrero JA,Wang Y,Blaufuss GS,Sandoval G,Fujikawa K,Cross AH,Russell JH,Cella M,Swat W

17-5812 was used in Flow cytometry/Cell sorting to propose a novel mechanism for control of T helper cell priming.

Thu Oct 28 00:00:00 UTC 2010

Stress-induced glucocorticoids at the earliest stages of herpes simplex virus-1 infection suppress subsequent antiviral immunity, implicating impaired dendritic cell function.

Journal of immunology (Baltimore, Md. : 1950)

Elftman MD,Hunzeker JT,Mellinger JC,Bonneau RH,Norbury CC,Truckenmiller ME

17-5812 was used in Flow cytometry/Cell sorting to investigate the specific mechanism for psychological stress-induced suppression of immune responses.

Mon Feb 15 00:00:00 UTC 2010

Lung interstitial macrophages alter dendritic cell functions to prevent airway allergy in mice.

The Journal of clinical investigation

Bedoret D,Wallemacq H,Marichal T,Desmet C,Quesada Calvo F,Henry E,Closset R,Dewals B,Thielen C,Gustin P,de Leval L,Van Rooijen N,Le Moine A,Vanderplasschen A,Cataldo D,Drion PV,Moser M,Lekeux P,Bureau F

17-5812 was used in Flow cytometry/Cell sorting to reveal a crucial role for IMs in maintaining immune homeostasis in the respiratory tract.

Tue Dec 01 00:00:00 UTC 2009

High frequencies of virus-specific CD8+ T-cell precursors.

Journal of virology

Seedhom MO,Jellison ER,Daniels KA,Welsh RM

17-5812 was used in Flow cytometry/Cell sorting to examine CD8(+) T-cell precursor frequencies for whole viruses.

Tue Dec 01 00:00:00 UTC 2009

生物信息学

蛋白别名: TCRVA; TCRVA2; V alpha 2; V alpha-2 TCR; Va 2; Va2; Valpha 2; Valpha2

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