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CD31 (PECAM-1) Antibody (25-0310-82) in Flow
Staining of rat splenocytes with Anti-Rat CD45R (B220) PE (Product # 12-0460-82) and 0.125 µg of Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (left) or 0.125 µg of Anti-Rat CD31 (PECAM-1) PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.
产品信息
25-0310-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Rat
已发表种属
Rat
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
TLD-3A12
偶联物
激发/发射光谱
569/780 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2573338
产品详细信息
Description: The TLD-3A12 monoclonal antibody reacts with rat CD31, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1) and gpIIa. This 130-kDa surface protein is expressed highly by endothelial cells but at low levels on all leukocytes and platelets. Studies have demonstrated that CD38 binds to CD31. Homotypic interaction of CD31 is important in adhesion, cell-cell and cell-matrix interactions, and signal transduction.
Applications Reported: This TLD-3A12 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This TLD-3A12 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: CD31; PECAM-1; Platelet endothelial cell adhesion molecule
基因别名: CD31; Pecam; Pecam1
UniProt ID: (Rat) Q3SWT0
Entrez Gene ID: (Rat) 29583
positive regulation of leukocyte migration
phagocytosis
cell adhesion
Rho protein signal transduction
regulation of cell migration
negative regulation of actin filament polymerization
response to cytokine
negative regulation of GTPase activity
wound healing
positive regulation of tyrosine phosphorylation of Stat5 protein
negative regulation of peptidyl-tyrosine phosphorylation
diapedesis
glomerular endothelium development
