Invitrogen

CD150 Monoclonal Antibody (mShad150), PE-Cyanine7, eBioscience™

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CD150 Antibody (25-1502-82) in Flow

Staining of C57Bl/6 splenocytes with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.25 µg of Rat IgG2b K Isotype Control PE-Cyanine7 (Product # 25-4031-82) (left) or 0.25 µg of Anti-Mouse CD150 PE-Cyanine7 (right). Total viable cells were used for analysis.

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产品信息

25-1502-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.5 µg/test

查看10篇已发表文章 10篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2b, lambda

分类

Monoclonal

类型

Antibody

克隆号

mShad150

偶联物

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

激发/发射光谱

569/780 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_10805742

产品详细信息

Description: The mShad150 monoclonal antibody reacts with mouse CD150, an ~70 kDa transmembrane glycoprotein also known as Signaling Lymphocyte Activation Molecule (SLAM). CD150 is expressed by T cells, in particular Th1, and B cells; this expression is rapidly upregulated upon activation. Immature thymocytes and dendritic cells also express this antigen. Signaling through SLAM in T cells induces proliferation and augmentation of the interferon-gamma response. Furthermore, SLAM is thought to play a role in adhesion between the T cell and antigen-presenting cell. The mShad150 antibody has been reported to also stain the CD150+CD244-CD48-CD41- population of pluripotent hematopoietic stem cells.

Applications Reported: This mShad150 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This mShad150 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD150 (SLAM, Signaling Lymphocyte Activation Molecule) belongs to the immunoglobulin gene superfamily, and is involved in T cell stimulation. SLAM is constitutively expressed on peripheral blood memory T cells, T cell clones, immature thymocytes, and a proportion of B cells, and is rapidly induced on naive T cells after activation. Activated B cells express the membrane bound form, the soluble form and the cytoplasmic isoforms of SLAM. It is suggested that signalling through homophilic SLAM-SLAM binding during B to B and B to T cell interactions enhances the expansion and differentiation of activated B cells. Reports suggest that the extracellular domain of CD150 is the receptor for the measles virus and acts as a co-activator on T cells and B cells. Moreover, CD150 is thought to interact with SH2D1A and with PTPN11 via its cytoplasmic domain. Mutations CD150 gene may be associated with X-linked lympho-proliferative disease (XLP).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

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25-1502-82 was used in Flow cytometry/Cell sorting to demonstrate that Phf6R274X plays a critical role in fine-tuning T cells and HSC homeostasis.

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Protocol for isolation and analysis of the leukemia stem cells in BCR-ABL-driven chronic myelogenous leukemia mice.

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25-1502-82 was used in flow cytometry to describe practical procedures for sorting and analysis of leukemia stem cells (LSCs) to improve our understanding of chronic myelogenous leukemia (CML).

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Activation of regulatory T cells triggers specific changes in glycosylation associated with Siglec-1-dependent inflammatory responses.

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Published figure using CD150 monoclonal antibody (Product # 25-1502-82) in Flow Cytometry

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Effects of long-term and brain-wide colonization of peripheral bone marrow-derived myeloid cells in the CNS.

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A Circular RNA Protects Dormant Hematopoietic Stem Cells from DNA Sensor cGAS-Mediated Exhaustion.

Immunity

Xia P,Wang S,Ye B,Du Y,Li C,Xiong Z,Qu Y,Fan Z

25-1502 was used in Flow cytometry/Cell sorting to study circular RNA-mediated maintenance of hematopoeitic stem cell homeostasis.

Tue Apr 17 00:00:00 UTC 2018

Gli1⁺ Mesenchymal Stromal Cells Are a Key Driver of Bone Marrow Fibrosis and an Important Cellular Therapeutic Target.

Cell stem cell

Schneider RK,Mullally A,Dugourd A,Peisker F,Hoogenboezem R,Van Strien PMH,Bindels EM,Heckl D,Büsche G,Fleck D,Müller-Newen G,Wongboonsin J,Ventura Ferreira M,Puelles VG,Saez-Rodriguez J,Ebert BL,Humphreys BD,Kramann R

25-1502 was used in Flow cytometry/Cell sorting to study the cellular origin of bone marrow fibrosis.

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Small molecule p300/catenin antagonist enhances hematopoietic recovery after radiation.

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Zhao Y,Wu K,Nguyen C,Smbatyan G,Melendez E,Higuchi Y,Chen Y,Kahn M

Published figure using CD150 monoclonal antibody (Product # 25-1502-82) in Flow Cytometry

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Recipient bone marrow assimilates the myeloid/lymphoid reconstitution of distinct fetal hematopoietic stem cells.

Oncotarget

Guo XL,Chu L,Ke F,Mu LL,Li Z,Cai JJ,Li HF,Hong DL

Published figure using CD150 monoclonal antibody (Product # 25-1502-82) in Flow Cytometry

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Sleep disruption impairs haematopoietic stem cell transplantation in mice.

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25-1502 was used in Flow cytometry/Cell sorting to provide insights into cellular and molecular mechanisms underlying the effects of sleep deprivation on HSCs.