Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 2
HA Tag Antibody (26183-D650) in ICC/IF
Immunofluorescent analysis of HA Tag was performed using 70% confluent HA-H3 transfected HEK-293E cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with HA Tag Monoclonal Antibody (2-2.2.14), DyLight 650 (Product # 26183-D650) at 1:200 dilution and Histone H3 Rabbit Polyclonal Antibody (Product # 711055) at 0.5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with ... View More
产品信息
26183-D650
应用
建议稀释比
已发表文章
产品规格
种属反应
Tag
已发表种属
Tag
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
2-2.2.14
抗原
HA peptide YPYDVPDYA derivitized to ovalbumin.
偶联物
激发/发射光谱
651/673 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
PBS with proprietary stabilizer
内含物
0.02% sodium azide
保存条件
4° C, do not freeze
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2533053
产品详细信息
26183-D650 has been successfully used for immunofluorescence. DyLight 650 has an excitation/emission of 652/672 nm.
HA Synthetic Peptide (Product # 26184) is available for use in neutralization and control experiments and to competitively elute HA-tagged fusion proteins from immobilized anti-HA affinity resin.
靶标信息
HA Tag (hemagglutinin tag) is an epitope tag that provides a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. HA (hemagglutinin) is a surface glycoprotein derived from influenza hemagglutinin, and is commonly expressed as a tag in conjunction with proteins to aid in identification and purification of the tagged protein for functional analysis. HA tag antibodies can be used to facilitate protein detection or co-immunopreciptation of tagged proteins.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: HA; HA Epitope Tag; HA Tag; HA-tag; hemagglutinin
