Invitrogen

P-cadherin Monoclonal Antibody (12H6)

P-cadherin Antibody in Immunohistochemistry (Frozen) (IHC (F))

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P-cadherin Antibody (32-4000) in IHC (F)

Immunohistochemistry analysis of P-Cadherin was performed on cryosections of human skin tissue. Tissues were blocked in 10% normal goat serum in 1X PBS containing 0.1% Triton X-100 (1X PBS-T) for 1 hour at room temperature (RT). The tissues were labeled with a P-Cadherin monoclonal antibody (clone 12H6, red, Product # 32-4000) diluted 1:50 in 3% normal goat serum in 1X PBS-T for 1 hour at RT, followed by de... View More

P-cadherin Antibody in Western Blot (WB)

P-cadherin Antibody in Immunohistochemistry (IHC)

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产品信息

32-4000

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-3 µg/mL

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

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免疫组化（冰冻） (IHC (F))

1:50

免疫细胞化学 (ICC/IF)

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其他PubMed (Misc)

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产品规格

种属反应

Dog, Human

已发表种属

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

12H6

抗原

Human P-Cadherin fusion protein.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533077

产品详细信息

32-4000 has successfully been used in western blot to detect P-cadherin in MDCK cell lysates.

靶标信息

P-cadherin, also known as Cadherin-3, is a Type 1 cadherin protein that belongs to the cadherin superfamily. Type 1 cadherins are single-pass transmembrane proteins that have 5 extracellular cadherin repeats and an intracellular domain that binds p120-catenin and beta-catenin. Cadherins are calcium-dependent cell-cell adhesion glycoproteins responsible for a range of processes including development, wound healing, cell-cell signaling, cell growth and differentiation. P-cadherin is expressed in human fetal structures, and in adult tissues such as the basal layer of the epidermis, breast, prostate, ovary, cervix, hair follicle, and corneal endothelium. P-cadherin expression has also been reported on embryonic stem cells, and stem cells of the normal mammary gland, and hair follicle. Overexpression of P-cadherin has been associated with poor prognosis in breast, prostate, ovary, colon, and stomach carcinomas. Mutations in CDH3 are associated with congential hypotrichosis with juvenile macular dystrophy.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Biphasic Squamoid Alveolar Renal Cell Carcinoma: A Distinctive Subtype of Papillary Renal Cell Carcinoma?

The American journal of surgical pathology

Hes O,Condom Mundo E,Peckova K,Lopez JI,Martinek P,Vanecek T,Falconieri G,Agaimy A,Davidson W,Petersson F,Bulimbasic S,Damjanov I,Jimeno M,Ulamec M,Podhola M,Sperga M,Pane Foix M,Shelekhova K,Kalusova K,Hora M,Rotterova P,Daum O,Pivovarcikova K,Michal M

324000 was used in immunohistochemistry - paraffin section to characterize 21 cases of biphasic squamoid alveolar renal cell carcinoma

Sun May 01 00:00:00 UTC 2016

HIV-1 Nef and KSHV oncogene K1 synergistically promote angiogenesis by inducing cellular miR-718 to regulate the PTEN/AKT/mTOR signaling pathway.

Nucleic acids research

Xue M,Yao S,Hu M,Li W,Hao T,Zhou F,Zhu X,Lu H,Qin D,Yan Q,Zhu J,Gao SJ,Lu C

Published figure using P-cadherin monoclonal antibody (Product # 32-4000) in Immunohistochemistry

Mon Sep 01 00:00:00 UTC 2014

Novel three-dimensional in vitro models of ovarian endometriosis.

Journal of ovarian research

Brueggmann D,Templeman C,Starzinski-Powitz A,Rao NP,Gayther SA,Lawrenson K

32-4000 was used in western blot to develop and characterize a novel three-dimensional cell culture model of ovarian endometriosis.

Thu Feb 06 00:00:00 UTC 2014

P-cadherin is a direct PAX3-FOXO1A target involved in alveolar rhabdomyosarcoma aggressiveness.

Oncogene

Thuault S,Hayashi S,Lagirand-Cantaloube J,Plutoni C,Comunale F,Delattre O,Relaix F,Gauthier-Rouvière C

32-4000 was used in immunocytochemistry and western blot to elucidate alveolar rhabdomyosarcoma pathogenesis.

Thu Apr 11 00:00:00 UTC 2013

P-cadherin is a direct PAX3-FOXO1A target involved in alveolar rhabdomyosarcoma aggressiveness.

Oncogene

Thuault S,Hayashi S,Lagirand-Cantaloube J,Plutoni C,Comunale F,Delattre O,Relaix F,Gauthier-Rouvière C

32-4000 was used in immunocytochemistry and western blot to elucidate alveolar rhabdomyosarcoma pathogenesis.

Thu Apr 11 00:00:00 UTC 2013

A three-dimensional microenvironment alters protein expression and chemosensitivity of epithelial ovarian cancer cells in vitro.

Laboratory investigation; a journal of technical methods and pathology

Lee JM,Mhawech-Fauceglia P,Lee N,Parsanian LC,Lin YG,Gayther SA,Lawrenson K

32-4000 was used in western blot to compare the histological, biological, and molecular features of 2D and 3D epithelial ovarian cancer cultures.

Wed May 01 00:00:00 UTC 2013

Sporadic hybrid oncocytic/chromophobe tumor of the kidney: a clinicopathologic, histomorphologic, immunohistochemical, ultrastructural, and molecular cytogenetic study of 14 cases.

Virchows Archiv : an international journal of pathology

Petersson F,Gatalica Z,Grossmann P,Perez Montiel MD,Alvarado Cabrero I,Bulimbasic S,Swatek A,Straka L,Tichy T,Hora M,Kuroda N,Legendre B,Michal M,Hes O

32-4000 was used in immunohistochemistry - paraffin section to study the clinicopathologic features of 14 cases of sporadic hybrid oncocytic/chromophobe tumor of the kidney

Thu Apr 01 00:00:00 UTC 2010

JNK signaling pathway is required for bFGF-mediated surface cadherin downregulation on HUVEC.

Experimental cell research

Wu JC,Yan HC,Chen WT,Chen WH,Wang CJ,Chi YC,Kao WY

32-4000 was used in western blot to find that basic fibroblast growth factor downregulates typical cadherin expression on the surface of human umbilical vein endothelial cells via FGF receptor 1 signaling

Fri Feb 01 00:00:00 UTC 2008

Differences in invasive capacity of endometrial cancer cell lines expressing different progesterone receptor isotypes: possible involvement of cadherins.

Journal of the Society for Gynecologic Investigation

Hanekamp EE,Gielen SC,De Ruiter PE,Chadha-Ajwani S,Huikeshoven FJ,Burger CW,Grootegoed JA,Blok LJ

32-4000 was used in western blot to assess if selective loss of progesterone receptor A or B affects the invasive capacity of endometrial cancer cells

Sun May 01 00:00:00 UTC 2005

生物信息学

蛋白别名: CADH3; cadherin 15, M-cadherin (myotubule); cadherin 3, type 1, P-cadherin (placental); Cadherin-3; calcium-dependent adhesion protein, placental; P-cadherin; Placental cadherin; Placental-cadherin

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基因别名: CDH3; CDHP; HJMD; PCAD

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UniProt ID: (Human) P22223

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Entrez Gene ID: (Dog) 611010, (Human) 1001

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功能

molecular_function calcium ion binding cadherin

参与通路