Invitrogen

CD115 (c-fms) Monoclonal Antibody (AFS98), Brilliant Ultra Violet™ 563, eBioscience™

CD115 (c-fms) Antibody in Flow Cytometry (Flow)

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CD115 (c-fms) Antibody (365-1152-82) in Flow

BALB/c mouse bone marrow cells were stimulated for 72 hours with Mouse M-CSF Recombinant Protein (Product # 14-8983-80). Cells were then stained with CD11b Monoclonal Antibody, eFluor 450 (Product # 48-0112-82) and 0.25 µg of Rat IgG2a kappa Isotype Control, Brilliant Ultra Violet 563 (Product # 365-4321-81) (left) or 0.25 µg of CD115 (c-fms) Monoclonal Antibody, Brilliant Ultra Violet 563 (right). Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50).

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产品信息

365-1152-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.5 µg/test

产品规格

种属反应

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

AFS98

偶联物

Brilliant Ultra Violet™ 563 Brilliant Ultra Violet™ 563 Brilliant Ultra Violet™ 563

激发/发射光谱

349/563 nm 查看光谱

形式

liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Description: The AFS98 monoclonal antibody reacts with the mouse CD115 molecule, a receptor for macrophage colony stimulating factor (M-CSF) or colony stimulating factor-1 (CSF-1). CD115 is expressed by monocyte, macrophage, osteoclast, and some epithelial cells. It is a 150 kDa c-fms gene product and belongs to immunoglobulin family. CSF-1 signaling through CSF-1R regulates the proliferation and differentiation of cells in the monocytic lineage.

Applications Reported: This AFS98 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This AFS98 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Brilliant Ultra Violet™ 563 (BUV563) is a tandem dye that emits at 564 nm and is intended for use on cytometers equipped with an ultraviolet (355 nm) laser. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone-specific performance should be determined empirically.

Excitation: 350 nm; Emission: 564 nm; Laser: Ultraviolet Laser.

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen.™

靶标信息

CSF1R (FMS) is involved in the production, differentiation, and function of macrophages, and is implicated in promyelocytic leukemias. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. CSF1R is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in the gene encoding CSF1R have been associated with a predisposition to myeloid malignancy.Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and plays an important role in innate immunity and in inflammatory processes. The first intron of the CSF1R gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction. Mutations in the CSF1R gene have been associated with a predisposition to myeloid malignancy.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: c-fms; CD115; CSF-1 receptor; CSF-1-R; CSFIR; EC 2.7.10.1; kinase CSFR; Macrophage colony-stimulating factor 1 receptor; OTTHUMP00000224093; Proto-oncogene c-Fms; proto-oncogene fms

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基因别名: AI323359; CD115; CSF-1R; Csf1r; Csfmr; Fim-2; Fms; M-CSF-R; M-CSFR

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UniProt ID: (Mouse) P09581

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Entrez Gene ID: (Mouse) 12978

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功能