Invitrogen

Tmem119 Monoclonal Antibody (V3RT1GOsz), Brilliant Ultra Violet™ 805, eBioscience™

Tmem119 Antibody in Flow Cytometry (Flow)

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Tmem119 Antibody (368-6119-82) in Flow

Demyelinated BALB/c mouse brain single cell suspension was stained with CD11b Monoclonal Antibody, APC (Product # 17-0112-82) and 0.5 µg of Rat IgG2a kappa Isotype Control, Brilliant Ultra Violet 805 (Product # 368-4321-81) (left) or 0.5 µg of Tmem119 Monoclonal Antibody, Brilliant Ultra Violet 805 (right). Total viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 506 (Product # 65-0866-18).

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产品信息

368-6119-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

1.0 µg/test

产品规格

种属反应

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

V3RT1GOsz

抗原

cell surface peptide

偶联物

Brilliant Ultra Violet™ 805 Brilliant Ultra Violet™ 805 Brilliant Ultra Violet™ 805

激发/发射光谱

349/804 nm 查看光谱

形式

liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Description: This V3RT1GOsz monoclonal antibody recognizes mouse Tmem119. This clone has been reported to be successfully used in flow cytometry.

Applications Reported: This V3RT1GOsz antibody has been reported for use in flow cytometric analysis.

Applications Tested: This V3RT1GOsz antibody has been tested by flow cytometric analysis of mouse brain cells after myelin removal. This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Brilliant Ultra Violet™ 805 (BUV805) is a tandem dye that emits at 797 nm and is intended for use on cytometers equipped with an ultraviolet (355 nm) laser. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright, Brilliant Violet, Brilliant Ultra Violet, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone-specific performance should be determined empirically.
Our internal testing suggests that Brilliant Ultra Violet™ 805 (BUV805) is compatible with short-term methanol-based fixation, but should not be stored in buffers containing methanol for longer than one hour.

Excitation: 355 nm; Emission: 797 nm; Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen.™

靶标信息

Tmem119 encodes a single-pass type I transmembrane protein which seems to interact with a multitude of micro-RNAs as well as certain transcription factors such as SMAD1, SMAD5 and RUNX2. Tmem119 has been identified as a surface marker of microglia that can be used to reliably distinguish microglia from infiltrating macrophages. Microglial cells are phagocytes that are restricted to the brain and involved in clearing up debris and dendrite pruning of neurons. They display a high functional plasticity, balancing neuroprotective and neurotoxic functions, which is reflected by their distinct physiology in response to changes in their microenvironment. Microglia are particularly mobile in areas of the brain undergoing restructuring. Reactive microglia co-express the marker Iba1. In a pathological context, microglia have been associated with differentiation of pathogenic/inflammatory A1 type astrocytes by secretion of Cq1, IL-1a and TNF when stimulated with LPS, and thought to contribute to the pathology of several neurodegenerative diseases such as Alzheimers, Parkinsons, Chorea Huntingtons disease and Multiple Sclerosis. The specific function Tmem119 plays on microglial cells remains unclear. Outside of the neural system, Tmem119 seems to be involved in osteoblast differentiation/ossification.

仅用于科研。不用于诊断过程。未经明确授权不得转售。