Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 4
4-1BB Ligand Antibody (MA5-29838) in ICC/IF
Immunofluorescence analysis of mouse 4-1BB Ligand (CD137L) was performed using 70% confluent log phase RAW 264.7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with 4-1BB Ligand Recombinant Rabbit Monoclonal Antibody (14) (Product # MA5-29838) at 1:100 di... View More
产品信息
MA5-29838
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
014
抗原
Recombinant Mouse TNFSF9 protein (Arg104-Glu309)
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
no preservative
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2785657
产品详细信息
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Mouse TNFSF9/4-1BBL.
靶标信息
CD137 ligand (CD137L) is a type II membrane protein and part of the TNF superfamily. CD137L is a co-stimulatory molecule that is expressed on antigen presenting cells (DCs, monocytes/macrophages, B cells) and is upregulated upon activation. Its receptor, CD137/4-1BB, is found on a variety of cells, including inflamed endothelial cells, where its expression enhances extravasation of CD137L expressing monocytes. Activated T cells also express CD137, and engagement with CD137L enhances T cell proliferation, IL-2 secretion, survival, and cytotoxic activity. CD137L is also expressed on a majority of B-cell lymphomas, including mantle cell lymphoma, follicular lymphoma, and diffuse large B-cell lymphoma (but it is lacking on Hodgkin lymphoma and T-cell lymphoma). CD137L is also expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interactions.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: 4 1 BB Ligand; 4 1 BBL; 4-1BB ligand; 4-1BBL; 41BB Ligand; 41BBL; CD 137 Ligand; CD 137L; CD137 Ligand; CDw137L; TNFRSF9; Tumor necrosis factor ligand superfamily member 9
基因别名: 4-1BB-L; 4-1BBL; AI848817; Cd137l; Cd157l; Ly63l; Tnfsf9
UniProt ID: (Mouse) P41274
Entrez Gene ID: (Mouse) 21950
immune response
cell-cell signaling
positive regulation of interferon-gamma production
positive regulation of interleukin-12 production
positive regulation of interleukin-6 production
positive regulation of activated T cell proliferation
regulation of cell proliferation
myeloid dendritic cell differentiation
positive regulation of cytotoxic T cell differentiation
