Invitrogen

Aurora A Monoclonal Antibody (35C1)

Aurora A Antibody in Immunocytochemistry (ICC/IF)

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Aurora A Antibody (45-8900) in ICC/IF

Immunofluorescent analysis of Aurora A Antibody (35C1)was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Aurora A Antibody (35C1) (Product # 45-8900) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Rabbit Anti-Mouse IgG Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 45 mi... View More

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产品信息

45-8900

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-3 µg/mL

查看5篇已发表文章 5篇已发表文章

免疫细胞化学 (ICC/IF)

1-2 µg/mL

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免疫沉淀 (IP)

5 µg

其他PubMed (Misc)

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产品规格

种属反应

Bovine, Dog, Horse, Human, Mouse, Non-human primate, Pig, Rat

已发表种属

Hamster, Human, Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

35C1

抗原

Recombinant protein derived from the full length of human Aurora A Kinase protein

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2533839

靶标信息

AURKA (Aurora A) is a serine/threonine kinase that regulates mitosis by association in vivo with centrosomes. Overexpression of AURKA is observed in many types of cancer. Aurora A plays a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. Aurora A plays a key role during tumor development and progression and is overexpressed in many human cancers including breast, ovarian and colorectal. Aurora A is viewed as a potential target for anticancer drug treatment. Tissue specificity: Highly expressed in testis and weakly in skeletal muscle, thymus and spleen.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The Aurora Kinase Inhibitor TAK901 Inhibits Glioblastoma Growth by Blocking SREBP1-Mediated Lipid Metabolism.

Cancers

Zhan X,Qiu R,He Y,Zhao Z,Huang M,Liu Q,Zhi F,Long W

45-8900 was used in Western Blotting to provide evidence that TAK901 inhibits GBM growth by suppressing SREBP1-mediated lipid metabolism.

Fri Nov 25 00:00:00 UTC 2022

iScience

Speight P,Rozycki M,Venugopal S,Szászi K,Kofler M,Kapus A

45-8900 was used in Western Blot to show that (1) both MRTF and SRF are indispensable for serum-induced PC resorption, and (2) they act via both transcriptional and local mechanisms.

Fri Jul 23 00:00:00 UTC 2021

iScience

Speight P,Rozycki M,Venugopal S,Szászi K,Kofler M,Kapus A

45-8900 was used in Western Blot to show that (1) both MRTF and SRF are indispensable for serum-induced PC resorption, and (2) they act via both transcriptional and local mechanisms.

Fri Jul 23 00:00:00 UTC 2021

Targeting Aurora A Kinase (AAK) in Platinum-Resistant High Grade Serous Ovarian Cancer.

Frontiers in oncology

Ganapathi RN,Norris EJ,Sutker AP,Klotz KE,Ganapathi MK

45-8900 was used in Western Blotting to show that in HGSOC without BRCA1/2 mutations, CP, or PT resistance can potentially be circumvented by sequential treatment with AL that inhibits AAK involved in G2-M transition.

Mon Sep 28 00:00:00 UTC 2020

Targeting Aurora A Kinase (AAK) in Platinum-Resistant High Grade Serous Ovarian Cancer.

Frontiers in oncology

Ganapathi RN,Norris EJ,Sutker AP,Klotz KE,Ganapathi MK

Mon Sep 28 00:00:00 UTC 2020

A novel mitosis-specific Cep215 domain interacts with Cep192 and phosphorylated Aurora A for organization of spindle poles.

Journal of cell science

Kuriyama R,Fisher CR

45-8900 was used in Immunocytochemistry to conclude that Cep215 plays a role in maintaining the structural integrity of the spindle pole by providing a platform for the molecules involved in centrosome maturation.

Tue Dec 29 00:00:00 UTC 2020

Quantitative Temporal Viromics of an Inducible HIV-1 Model Yields Insight to Global Host Targets and Phospho-Dynamics Associated with Protein Vpr.

Molecular & cellular proteomics : MCP

Lapek JD,Lewinski MK,Wozniak JM,Guatelli J,Gonzalez DJ

45-8900 was used in Western Blotting to define mechanisms of HIV viral protein action by identifying host proteins whose abundance is affected by Vpr, and the corresponding modulation of down-stream signalling pathways, specifically those regulated by phosphorylation.

Tue Aug 01 00:00:00 UTC 2017

Aurora-A mediated histone H3 phosphorylation of threonine 118 controls condensin I and cohesin occupancy in mitosis.

eLife

Wike CL,Graves HK,Hawkins R,Gibson MD,Ferdinand MB,Zhang T,Chen Z,Hudson DF,Ottesen JJ,Poirier MG,Schumacher J,Tyler JK

45-8900 was used in western blot to examine how histone H3 threonine 118 alters chromosome structure

Tue Feb 16 00:00:00 UTC 2016

14-3-3γ Prevents Centrosome Amplification and Neoplastic Progression.

Scientific reports

Mukhopadhyay A,Sehgal L,Bose A,Gulvady A,Senapati P,Thorat R,Basu S,Bhatt K,Hosing AS,Balyan R,Borde L,Kundu TK,Dalal SN

45-8900 was used in western blot to analyze prevention of centrosome amplification and neoplastic progression by 14-3-3gamma

Thu Jun 02 00:00:00 UTC 2016

生物信息学

蛋白别名: ARK-1; AURAK; Aurora 2; aurora A; Aurora A Kinase; aurora family kinase 1; Aurora kinase A; Aurora-A; aurora-related kinase 1; aurora/IP; aurora/IPL1-like kinase; Aurora/IPL1-related kinase 1; AURORA2; Breast tumor-amplified kinase; EC 2.7.11.1; hARK1; Ipl1- and aurora-related kinase 1; IPL1-related kinase; MGC34538; OTTHUMP00000031341; OTTHUMP00000166072; protein phosphatase 1, regulatory subunit 47; ratAurA; RP5-1167H4.6; sereine threonine kinase; serine/threonine kinase 6; serine/threonine protein kinase 15; serine/threonine protein kinase 6; Serine/threonine-protein kinase 15; Serine/threonine-protein kinase 6; Serine/threonine-protein kinase aurora-A; Serine/threonine-protein kinase Ayk1

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基因别名: AIK; AIRK1; ARK-1; ARK1; AU019385; AURA; AURKA; Aurora-A; AW539821; AYK1; BTAK; IAK; IAK1; PPP1R47; STK15; STK6; STK7