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KLRG1 Antibody (46-9488-42) in Flow
LEFT: Staining of normal human peripheral blood cells with Anti-Human CD28 eFluor® 450 (Product # 48-0289-42) and Anti-Human KLRG1 PerCP-eFluor® 710. CD8+ cells in the lymphocyte gate were used for analysis. RIGHT: Staining of normal human peripheral blood cells with Anti-Human CD56 (NCAM) PE-eFluor® 610 (Product # 61-0567-42) and Anti-Human KLRG1 PerCP-eFluor® 710. Cells in the lymphocyte gate were used for analysis.
产品信息
46-9488-42
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Not Applicable
宿主/亚型
Mouse
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
13F12F2
偶联物
激发/发射光谱
482/708 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with 0.2% BSA
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2573889
产品详细信息
Description: This 13F12F2 monoclonal antibody reacts with human killer cell lectin-like receptor subfamily G, member 1 (KLRG1), a type II transmembrane inhibitor receptor of the C-type lectin superfamily that contains an ITIM domain. This inhibitory receptor is expressed on subsets of gamma-delta T cells, NK (CD56dim), CD8+ and CD4+ T Cells. KLRG1 is expressed primarily by cells with an effector/memory phenotype that are short-lived, but capable of immediate effector cell function. Cadherin-E, -N, and -R are ligands for KLRG1. Cadherin/KLRG1 interaction inhibits cytolytic activity and proliferation. The percentage of KLRG1 positive cells can vary considerably, depending on antigen experience.
The clones 13F12F2 and 13A2 appear to recognize a similar epitope based on cross-blocking studies.
Applications Reported: This 13F12F2 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 13F12F2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
KLRG1 (Killer cell lectin-like receptor G1, MAFA, 2F1-Ag) is a homodimeric member of the lectin-like type 2 transmembrane receptor family whose members contain characteristic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in their cytoplasmic domains. These ITIMs interact with the SH2 domains of protein phosphatases such as SHP-1. KLRG1 is an inhibitory receptor that is expressed on natural killer (NK) cells and certain T cells. NK cells are involved in the lysis of tumor cells and virus-infected cells, and mediate humoral and cell-mediated immune responses.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: C-type lectin domain family 15 member A; C-type lectin domain family 15, member A; ITIM-containing receptor MAFA-L; Killer cell lectin-like receptor subfamily G member 1; killer cell lectin-like receptor subfamily G, member 1; MAFA-like receptor; Mast cell function-associated antigen; mast cell function-associated antigen (ITIM-containing); natural killer cell receptor
基因别名: 2F1; CLEC15A; KLRG1; MAFA; MAFA-2F1; MAFA-L; MAFA-LIKE; MAFAL
UniProt ID: (Human) Q96E93
Entrez Gene ID: (Human) 10219
