Invitrogen

EOMES Monoclonal Antibody (WD1928), APC-eFluor™ 780, eBioscience™

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EOMES Antibody (47-4877-42) in Flow

Normal human peripheral blood cells were surfaced stained with Anti-Human CD56 (NCAM) PE (Product # 12-0567-42), then intracellularly stained with Mouse IgG1 K Isotype Control APC-eFluor® 780 (Product # 47-4714-82) (left) or Anti-Human EOMES APC-eFluor® 780 (right) using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Cells in the lymphocyte gate were used for analysis.

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产品信息

47-4877-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.03 µg)/test

查看15篇已发表文章 15篇已发表文章

产品规格

种属反应

Human, Pig

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

WD1928

偶联物

APC-eFluor™ 780 APC-eFluor™ 780 APC-eFluor™ 780

激发/发射光谱

756/785 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2573982

产品详细信息

Description: This WD1928 antibody recognizes Eomesodermin (Eomes), also known as T-box brain 2 (TBR2). Eomes is a T-box transcription factor that is highly homologous to T-bet, which is essential during trophoblast development and gastrulation in most vertebrates. In the immune system, Eomes controls the differentiation of effector and memory CD8+ T cells, as well as natural killer (NK) cells. Expression of Eomes in these cells correlates with high expression of CD122, the common beta-chain of the IL-2R and IL-15R.

Applications Reported: This WD1928 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This WD1928 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Refer to Best Protocols for Staining Protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

APC-eFluor® 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

TBR2 is a member of a conserved protein family that shares a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. A similiar protein disrupted in mice is shown to be essential during trophoblast development and gastrulation. TBR2 plays a role in brain development and is required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

A single-cell map of dynamic chromatin landscapes of immune cells in renal cell carcinoma.

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Kourtis N,Wang Q,Wang B,Oswald E,Adler C,Cherravuru S,Malahias E,Zhang L,Golubov J,Wei Q,Lemus S,Ni M,Ding Y,Wei Y,Atwal GS,Thurston G,Macdonald LE,Murphy AJ,Dhanik A,Sleeman MA,Tykodi SS,Skokos D

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Transcriptional profiling of human Vδ1 T cells reveals a pathogen-driven adaptive differentiation program.

Cell reports

McMurray JL,von Borstel A,Taher TE,Syrimi E,Taylor GS,Sharif M,Rossjohn J,Remmerswaal EBM,Bemelman FJ,Vieira Braga FA,Chen X,Teichmann SA,Mohammed F,Berry AA,Lyke KE,Williamson KC,Stubbington MJT,Davey MS,Willcox CR,Willcox BE

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Homeostatic Function and Inflammatory Activation of Ileal CD8⁺ Tissue-Resident T Cells Is Dependent on Mucosal Location.

Cellular and molecular gastroenterology and hepatology

Lutter L,Roosenboom B,Brand EC,Ter Linde JJ,Oldenburg B,van Lochem EG,Horjus Talabur Horje CS,van Wijk F

47-4877-42 was used in Flow Cytometry to indicate that therapeutic strategies could have a different impact on the same immune cells depending on the local compartment and presence of an inflammatory milieu, and should be taken into account when investigating short- and long-term effects of new gut T cell-targeting drugs.

Mon Mar 28 00:00:00 UTC 2022

Homeostatic Function and Inflammatory Activation of Ileal CD8⁺ Tissue-Resident T Cells Is Dependent on Mucosal Location.

Cellular and molecular gastroenterology and hepatology

Lutter L,Roosenboom B,Brand EC,Ter Linde JJ,Oldenburg B,van Lochem EG,Horjus Talabur Horje CS,van Wijk F

Mon Mar 28 00:00:00 UTC 2022

Culture, expansion, and flow-cytometry-based functional analysis of pteropid bat MR1-restricted unconventional T cells.

STAR protocols

Sia WR,Hey YY,Foo R,Wang LF,Leeansyah E

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Norovirus-Specific CD8⁺ T Cell Responses in Human Blood and Tissues.

Cellular and molecular gastroenterology and hepatology

Pattekar A,Mayer LS,Lau CW,Liu C,Palko O,Bewtra M,Consortium H,Lindesmith LC,Brewer-Jensen PD,Baric RS,Betts MR,Naji A,Wherry EJ,Tomov VT

Published figure using EOMES monoclonal antibody (Product # 47-4877-42) in Flow Cytometry

Tue Mar 08 00:00:00 UTC 2022

Cell-type-specific 3D epigenomes in the developing human cortex.

Nature

Song M,Pebworth MP,Yang X,Abnousi A,Fan C,Wen J,Rosen JD,Choudhary MNK,Cui X,Jones IR,Bergenholtz S,Eze UC,Juric I,Li B,Maliskova L,Lee J,Liu W,Pollen AA,Li Y,Wang T,Hu M,Kriegstein AR,Shen Y

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Complement opsonization of HIV affects primary infection of human colorectal mucosa and subsequent activation of T cells.

eLife

Bhattacharya P,Ellegård R,Khalid M,Svanberg C,Govender M,Keita ÅV,Söderholm JD,Myrelid P,Shankar EM,Nyström S,Larsson M

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CD19-CAR-T Cells Bearing a KIR/PD-1-Based Inhibitory CAR Eradicate CD19⁺HLA-C1^- Malignant B Cells While Sparing CD19⁺HLA-C1⁺ Healthy B Cells.

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Tao L,Farooq MA,Gao Y,Zhang L,Niu C,Ajmal I,Zhou Y,He C,Zhao G,Yao J,Liu M,Jiang W

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The CD38/NAD/SIRTUIN1/EZH2 Axis Mitigates Cytotoxic CD8 T Cell Function and Identifies Patients with SLE Prone to Infections.

Cell reports

Katsuyama E,Suarez-Fueyo A,Bradley SJ,Mizui M,Marin AV,Mulki L,Krishfield S,Malavasi F,Yoon J,Sui SJH,Kyttaris VC,Tsokos GC

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C reactive protein impairs adaptive immunity in immune cells of patients with melanoma.

Journal for immunotherapy of cancer

Yoshida T,Ichikawa J,Giuroiu I,Laino AS,Hao Y,Krogsgaard M,Vassallo M,Woods DM,Stephen Hodi F,Weber J

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Scientific reports

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The Obese Liver Environment Mediates Conversion of NK Cells to a Less Cytotoxic ILC1-Like Phenotype.

Frontiers in immunology

Cuff AO,Sillito F,Dertschnig S,Hall A,Luong TV,Chakraverty R,Male V

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Unique transcriptional and protein-expression signature in human lung tissue-resident NK cells.

Nature communications

Marquardt N,Kekäläinen E,Chen P,Lourda M,Wilson JN,Scharenberg M,Bergman P,Al-Ameri M,Hård J,Mold JE,Ljunggren HG,Michaëlsson J

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Mon Aug 26 00:00:00 UTC 2019

Tissue-resident Eomes⁺ NK cells are the major innate lymphoid cell population in human infant intestine.

Nature communications

Sagebiel AF,Steinert F,Lunemann S,Körner C,Schreurs RRCE,Altfeld M,Perez D,Reinshagen K,Bunders MJ

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Thu Feb 28 00:00:00 UTC 2019

Analysis of a Jup hypomorphic allele reveals a critical threshold for postnatal viability.

Genesis (New York, N.Y. : 2000)

Swope D,Li J,Muller EJ,Radice GL

Published figure using EOMES monoclonal antibody (Product # 47-4877-42) in Flow Cytometry

Mon Oct 01 00:00:00 UTC 2012

生物信息学

蛋白别名: Eomesodermin homolog; T-box brain protein 2; T-brain-2

查看更多收起

基因别名: EOMES; TBR2

查看更多收起

UniProt ID: (Human) O95936

查看更多收起

Entrez Gene ID: (Human) 8320, (Pig) 100622125

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功能

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II activating transcription factor binding transcriptional repressor activity, RNA polymerase II transcription factor binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding sequence-specific DNA binding Rel homology transcription factor

参与通路

negative regulation of transcription from RNA polymerase II promoter endoderm formation mesoderm formation endodermal cell fate specification trophectodermal cell differentiation adaptive immune response CD8-positive, alpha-beta T cell differentiation involved in immune response transcription, DNA-templated brain development cardioblast differentiation stem cell population maintenance olfactory bulb development cerebral cortex regionalization cerebral cortex neuron differentiation interferon-gamma production skeletal muscle cell differentiation positive regulation of cell differentiation regulation of neuron differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter cell differentiation involved in embryonic placenta development mesodermal to mesenchymal transition involved in gastrulation

EOMES Monoclonal Antibody (WD1928), APC-eFluor™ 780, eBioscience™

EOMES Antibody (47-4877-42) in Flow

产品信息

47-4877-42

流式细胞分析 (Flow)

种属反应

已发表种属

宿主/亚型

推荐的同型对照

分类

类型

克隆号

偶联物

激发/发射光谱

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

How to use the Panel Builder

生物信息学

性能保障

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