Invitrogen

CD8a Monoclonal Antibody (SK1), eFluor™ 450, eBioscience™

图: 1 / 8

CD8a Antibody (48-0087-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD19 APC (Product # 17-0199-42) and Mouse IgG1 K Isotype Control eFluor® 450 (Product # 48-4714-82) (left) or Anti-Human CD8a eFluor® 450 (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

产品信息

48-0087-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.125 µg)/test

查看14篇已发表文章 14篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

SK1

偶联物

eFluor™ 450 eFluor™ 450 eFluor™ 450

激发/发射光谱

405/445 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_11149305

产品详细信息

Description: The SK1 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells.

Applications Reported: This SK1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This SK1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

RASA2 ablation in T cells boosts antigen sensitivity and long-term function.

Nature

Carnevale J,Shifrut E,Kale N,Nyberg WA,Blaeschke F,Chen YY,Li Z,Bapat SP,Diolaiti ME,O'Leary P,Vedova S,Belk J,Daniel B,Roth TL,Bachl S,Anido AA,Prinzing B,Ibañez-Vega J,Lange S,Haydar D,Luetke-Eversloh M,Born-Bony M,Hegde B,Kogan S,Feuchtinger T,Okada H,Satpathy AT,Shannon K,Gottschalk S,Eyquem J,Krenciute G,Ashworth A,Marson A

Published figure using CD8a monoclonal antibody (Product # 48-0087-42) in Flow Cytometry

Thu Sep 01 00:00:00 UTC 2022

Cancer-secreted exosomal miR-1468-5p promotes tumor immune escape via the immunosuppressive reprogramming of lymphatic vessels.

Molecular therapy : the journal of the American Society of Gene Therapy

Zhou C,Wei W,Ma J,Yang Y,Liang L,Zhang Y,Wang Z,Chen X,Huang L,Wang W,Wu S

48-0087-42 was used in Flow Cytometry to investigate how lymphatic endothelial cells are immunosuppressed to allow cancer immune evasion.

Wed Apr 07 00:00:00 UTC 2021

Exosome-derived miR-142-5p remodels lymphatic vessels and induces IDO to promote immune privilege in the tumour microenvironment.

Cell death and differentiation

Zhou C,Zhang Y,Yan R,Huang L,Mellor AL,Yang Y,Chen X,Wei W,Wu X,Yu L,Liang L,Zhang D,Wu S,Wang W

48-0087 was used in Flow cytometry/Cell sorting to suggest that LECs act as an integral component of the immune checkpoint(s) in the TME and may serve as a potential new target for CSCC diagnosis and treatment.

Mon Feb 01 00:00:00 UTC 2021

Exosome-derived miR-142-5p remodels lymphatic vessels and induces IDO to promote immune privilege in the tumour microenvironment.

Cell death and differentiation

Zhou C,Zhang Y,Yan R,Huang L,Mellor AL,Yang Y,Chen X,Wei W,Wu X,Yu L,Liang L,Zhang D,Wu S,Wang W

Mon Feb 01 00:00:00 UTC 2021

SARS-CoV-2 infection paralyzes cytotoxic and metabolic functions of the immune cells.

Heliyon

Singh Y,Trautwein C,Fendel R,Krickeberg N,Berezhnoy G,Bissinger R,Ossowski S,Salker MS,Casadei N,Riess O

Published figure using CD8a monoclonal antibody (Product # 48-0087-42) in Flow Cytometry

Tue Jun 01 00:00:00 UTC 2021

PAX8 lineage-driven T cell engaging antibody for the treatment of high-grade serous ovarian cancer.

Scientific reports

Lee E,Szvetecz S,Polli R,Grauel A,Chen J,Judge J,Jaiswal S,Maeda R,Schwartz S,Voedisch B,Piksa M,Japutra C,Sadhasivam L,Wang Y,Carrion A,Isim S,Liang J,Nicholson T,Lei H,Fang Q,Steinkrauss M,Walker D,Wagner J,Cremasco V,Wang HQ,Galli GG,Granda B,Mansfield K,Simmons Q,Nguyen AA,Vincent Jordan N

Published figure using CD8a monoclonal antibody (Product # 48-0087-42) in Flow Cytometry

Wed Jul 21 00:00:00 UTC 2021

FS222, a CD137/PD-L1 Tetravalent Bispecific Antibody, Exhibits Low Toxicity and Antitumor Activity in Colorectal Cancer Models.

Clinical cancer research : an official journal of the American Association for Cancer Research

Lakins MA,Koers A,Giambalvo R,Munoz-Olaya J,Hughes R,Goodman E,Marshall S,Wollerton F,Batey S,Gliddon D,Tuna M,Brewis N

48-0087 was used in Flow cytometry/Cell sorting to develop FS222, a bispecific tetravalent antibody targeting CD137 and PD-L1, to induce T-cell activation to eradicate tumors without the current toxicity and efficacy limitations seen in the clinic.

Sat Aug 01 00:00:00 UTC 2020

Synthetic Abortive HIV-1 RNAs Induce Potent Antiviral Immunity.

Frontiers in immunology

Stunnenberg M,Sprokholt JK,van Hamme JL,Kaptein TM,Zijlstra-Willems EM,Gringhuis SI,Geijtenbeek TBH

Published figure using CD8a monoclonal antibody (Product # 48-0087-42) in Flow Cytometry

Fri Feb 19 00:00:00 UTC 2021

DNA hypomethylation promotes transposable element expression and activation of immune signaling in renal cell cancer.

JCI insight

de Cubas AA,Dunker W,Zaninovich A,Hongo RA,Bhatia A,Panda A,Beckermann KE,Bhanot G,Ganesan S,Karijolich J,Rathmell WK

48-0087 was used in Flow cytometry/Cell sorting to identify a potential strategy to induce TE expression through inhibition of DNA methylation in modulating T cell action via regulation of the innate antiviral pathway.

Thu Jun 04 00:00:00 UTC 2020

Cytotoxic effects of ex vivo-expanded natural killer cell-enriched lymphocytes (MYJ1633) against liver cancer.

BMC cancer

Choi JW,Lee ES,Kim SY,Park SI,Oh S,Kang JH,Ryu HA,Lee S

Published figure using CD8a monoclonal antibody (Product # 48-0087-42) in Flow Cytometry

Mon Aug 19 00:00:00 UTC 2019

Systematic testing and specificity mapping of alloantigen-specific chimeric antigen receptors in regulatory T cells.

JCI insight

Dawson NA,Lamarche C,Hoeppli RE,Bergqvist P,Fung VC,McIver E,Huang Q,Gillies J,Speck M,Orban PC,Bush JW,Mojibian M,Levings MK

48-0087 was used in Flow cytometry/Cell sorting to characterise and test alloantigen-specific chimeric antigen receptors in regulatory T cells.

Thu Mar 21 00:00:00 UTC 2019

Reduced monocyte and macrophage TNFSF15/TL1A expression is associated with susceptibility to inflammatory bowel disease.

PLoS genetics

Richard AC,Peters JE,Savinykh N,Lee JC,Hawley ET,Meylan F,Siegel RM,Lyons PA,Smith KGC

48-0087 was used in Flow cytometry/Cell sorting to indicate that genetically enhanced expression of TNFSF15 in specific cell types may confer protection against the development of IBD.

Sat Sep 01 00:00:00 UTC 2018

Ex vivo culture of human atherosclerotic plaques: A model to study immune cells in atherogenesis.

Atherosclerosis

Lebedeva A,Vorobyeva D,Vagida M,Ivanova O,Felker E,Fitzgerald W,Danilova N,Gontarenko V,Shpektor A,Vasilieva E,Margolis L

Published figure using CD8a monoclonal antibody (Product # 48-0087-42) in Flow Cytometry

Fri Dec 01 00:00:00 UTC 2017

CD8⁺ T cells specific for the islet autoantigen IGRP are restricted in their T cell receptor chain usage.

Scientific reports

Fuchs YF,Eugster A,Dietz S,Sebelefsky C,Kühn D,Wilhelm C,Lindner A,Gavrisan A,Knoop J,Dahl A,Ziegler AG,Bonifacio E

48-0087 was used in Flow cytometry/Cell sorting to examine whether there was expansion of clonotypes and sharing of T cell receptor chains in autoreactive CD8+ T cell repertoires.

Thu Mar 16 00:00:00 UTC 2017

Alloantigen-specific regulatory T cells generated with a chimeric antigen receptor.

The Journal of clinical investigation

MacDonald KG,Hoeppli RE,Huang Q,Gillies J,Luciani DS,Orban PC,Broady R,Levings MK

48-0087 was used in Flow cytometry/Cell sorting to investigate alloantigen-specific regulatory T cells generated with a chimeric antigen receptor.

Fri Apr 01 00:00:00 UTC 2016

生物信息学

蛋白别名: CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen

查看更多收起

基因别名: CD8; CD8A; CD8B; CD8B1; LEU2; LY3; LYT3; MAL; p32; P37

查看更多收起

UniProt ID: (Human) P01732, (Human) P10966

查看更多收起

Entrez Gene ID: (Human) 925, (Human) 926

查看更多收起

功能

protein binding coreceptor activity protein kinase binding MHC class I protein binding protein homodimerization activity defense/immunity protein immunoglobulin receptor superfamily

参与通路