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Invitrogen
CD365 (Tim-1) Recombinant Rabbit Monoclonal Antibody (P365D), Alexa Fluor™ 488, eBioscience™
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CD365 (Tim-1) Antibody (53-3658-42) in Flow
Antibody specificity was demonstrated by siRNA mediated knockdown of CD365. 769P cells were transfected with either non-targeting control siRNA or siRNA against CD365. As expected, loss of signal was observed with CD365 clone P365D in cells transfected with the CD365 specific siRNA (pink histogram) but not the cells transfected with control siRNA (purple histogram). SI denotes the staining index calculated against corresponding isotype controls.
产品信息
53-3658-42
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
P365D
抗原
Protein corresponding to Human CD365 (aa22-aa129)
偶联物
激发/发射光谱
499/520 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Protein A
保存液
proprietary buffer/PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Wet ice
RRID
AB_2848432
产品详细信息
Description: This P365D recombinant rabbit monoclonal antibody reacts with human CD365, which is also known as Tim-1, Kim-1, or Havcr1.
Applications Tested: This P365D antibody has been pre-diluted and tested by flow cytometric analysis of 769P cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
CD365 also known as T-cell immunoglobulin and mucin domain 1 (Tim-1) is a type 1 surface glycoprotein that consists of extracellular N-terminal immunoglobulin V-like domain and a mucin domain, and a short cytoplasmic tail. The endogenous ligand for CD365 is Tim-4. In addition, CD365 is known to be a cellular receptor or co-receptor for a number of viruses including hepatitis A and C viruses, Ebola virus, Marburg virus, Dengue virus, Zika virus, West Nile virus, and Japanese encephalitis virus. The direct binding of the viruses by CD365 occurs via phosphatildyl-serine exposed on viral envelopes. Polymorphisms within human CD365 gene (HAVCR1) are associated with susceptibility to infection and the disease severity. CD365 has also been implicated in the T cell co-stimulation. CD365 is primarily expressed in epithelial cells. It is also highly expressed in A549, 769-P and Caco-2 and cell lines. CD365 exists also as a soluble protein generated by metalloproteinase mediated proteolytic cleavage of the surface protein.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CD365; HAVcr-1; Hepatitis A virus cellular receptor 1; Kidney injury molecule 1; KIM-1; T cell immunoglobin domain and mucin domain protein 1; T-cell immunoglobulin and mucin domain-containing protein 1; T-cell immunoglobulin mucin family member 1; T-cell immunoglobulin mucin receptor 1; T-cell membrane protein 1; TIM; TIMD-1
基因别名: CD365; HAVCR; HAVCR-1; HAVCR1; KIM-1; KIM1; TIM; TIM-1; TIM1; TIMD-1; TIMD1
UniProt ID: (Human) Q96D42
Entrez Gene ID: (Human) 26762
