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Invitrogen
alpha-Fetoprotein Monoclonal Antibody (AFP3), Alexa Fluor™ 488, eBioscience™
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alpha-Fetoprotein Antibody (53-6583-82) in ICC/IF
Immunofluorescence analysis of AFP was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with AFP Mouse Monoclonal Antibody (Product # 53-6583-82) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight ... View More
产品信息
53-6583-82
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
AFP3
偶联物
激发/发射光谱
499/520 nm
查看光谱
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_10548900
产品详细信息
Description: This AFP3 monoclonal antibody reacts with human alpha-fetoprotein (AFP). This 70-kDa secretory protein is a member of the albumin gene family. Synthesized by the yolk sac and fetal liver during embryogenesis, AFP protein levels are highest in fetal serum. After birth, serum AFP levels decrease dramatically. In fact, AFP is nearly undetectable in normal adult serum. However, hepatocellular carcinoma and germ cell teratoblastoma, as well as liver regeneration, viral hepatitis, and cirrhosis, leads to elevated AFP serum levels in adults. As such, detection of this protein is frequently used as a diagnostic marker for these conditions.
When performing western blotting or immunohistochemistry on paraffin section, we recommend the use of monoclonal antibody 1E8 (Product # 14-9499).
Applications Reported: This AFP3 antibody has been reported for use in immunocytochemistry.
Applications Tested: This AFP3 antibody has been tested by immunofluorescent staining of formaldehyde-fixed and permeabilized HepG2 cells. This can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
AFP (Alpha Fetoprotein) is a major plasma protein produced by the yolk sac, intestinal tract and the liver during fetal life. AFP expression in adults is often associated with hepatoma or teratoma. However, hereditary persistence of AFP may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and AFP and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. AFP is found in monomeric, dimeric, and trimeric forms. AFP can also bind to copper ions, nickel ions, fatty acids and bilirubin. The level of AFP in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. High level of serum AFP has been identified in patients with hepatocellular carcinomas (HCC), teratoblastoma, colorectal cancer, pancreatic cancer, and germ cell neoplasms.
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生物信息学
蛋白别名: a fetoglobulin; a fetoprotein; Alpha fetoglobulin; Alpha-1-fetoprotein; Alpha-fetoglobulin; Alpha-fetoprotein; fetoglobulin; fetoprotein; α fetoglobulin; α fetoprotein
基因别名: AFP; AFPD; FETA; HPAFP
UniProt ID: (Human) P02771
Entrez Gene ID: (Human) 174
