Invitrogen

CD24 Monoclonal Antibody (M1/69), Alexa Fluor™ 532, eBioscience™

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CD24 Antibody (58-0242-82) in Flow

C57BL/6 mouse splenocytes were stained with CD3 Monoclonal Antibody, eFluor 450 (Product # 48-0032-82) and 0.125 µg of Rat IgG2b kappa Isotype Control, Alexa Fluor 532 (Product # 58-4031-80) (left) or 0.125 µg of CD24 Monoclonal Antibody, Alexa Fluor 532 (right). Cells in the lymphocyte gate were used for analysis.

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产品信息

58-0242-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.25 µg/test

查看10篇已发表文章 10篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Not Applicable

宿主/亚型

Rat / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

M1/69

偶联物

Alexa Fluor™ 532 Alexa Fluor™ 532 Alexa Fluor™ 532

激发/发射光谱

534/553 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2815268

产品详细信息

Description: The M1/69 monoclonal antibody reacts with the mouse CD24 molecule, also known as Heat Stable Antigen (HSA). This 35-50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes and myeloid lineage. CD24 is a variably glycosylated molecule resulting in heterogeneity of molecular mass of this antigen on cells of different lineages and antibodies to CD24 exhibit subtle differences in staining level on lymphocyte populations. The expression of CD24 has been used to resolve stages of B lymphopoiesis in mouse bone marrow. It has been reported that P-selectin (CD62P) binds to CD24.

Applications Reported: This M1/69 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This M1/69 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Alexa Fluor 532 is excited with the Green laser (532 nm) and emits at 561 nm. This cannot be used with the Yellow-Green laser (561 nm). We recommend using a 560/14 band pass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 532 nm; Emission: 561 nm; Laser: Green Laser

靶标信息

CD24 is a two-chain glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed at multiple stages of B-cell development, beginning with the bone marrow pro-B-cell compartment and continuing through mature, surface Ig positive B-cells. Plasma cell expression is very low or negative. CD24 is also expressed on the majority of B-lineage acute lymphoblastic leukemias, B-cell CCLs and B-cell non-Hodgkin's lymphomas. CD24 may play a role in regulation of B-cell proliferation and maturation, and control of autoimmunity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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