Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 5
5HT1B R Antibody (OSS00182W-100UL) in IHC (P)
IHC-P on paraffin sections of mouse spleen. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 mL 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instruction... View More
Product Details
OSS00182W-100UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Not Applicable
Host/Isotype
Rabbit
/ Ig
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide from mouse 5HT1B receptor conjugated to blue carrier protein was used as the antigen
Conjugate
Form
Lyophilized
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Specificity of this antibody: 5HT1B receptor.
Target Information
Serotonin (5-hydroxytryptamine, 5-HT), originally discovered as a serum factor plays important roles in regulating diverse biological processes in central and peripheral nervous systems, cardiovascular systems, and gastrointestinal systems. Molecular cloning studies have identified 5 receptors belonging to the 5-HT1 subtypes. These receptors are negatively coupled to adenylyl cyclase. Radioligand binding studies suggest the presence of 5-HT1B (formerly known as 5-HT1DbR) sites in substantia nigra, dorsal subiculum, globus pallidus, and superior collicum. In situ hybridization studies show abundance of 5-HT1BR mRNA in CA1 pyramidal cells of hippocampus, Purkinje cells of cerebellum, olfactory tubercle, subthalamic nucleus and somewhat lower levels in the caudate-putamen, cortical areas, thalamic and hypothalamic nuclei.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 5-HT-1B; 5-HT1B receptor; 5-HT<1B> receptor; 5-hydroxytryptamine receptor 1B; 5HT1B Receptor; Serotonin receptor 1B
Gene Aliases: 5ht1b; Htr1b
UniProt ID: (Mouse) P28334
Entrez Gene ID: (Mouse) 15551
G-protein coupled receptor internalization
signal transduction
G-protein coupled receptor signaling pathway
adenylate cyclase-inhibiting serotonin receptor signaling pathway
protein kinase C-activating G-protein coupled receptor signaling pathway
serotonin receptor signaling pathway
chemical synaptic transmission
behavior
feeding behavior
response to toxic substance
regulation of dopamine secretion
negative regulation of serotonin secretion
negative regulation of cAMP biosynthetic process
negative regulation of synaptic transmission, GABAergic
cellular response to drug
response to cocaine
vasoconstriction
response to drug
drinking behavior
response to ethanol
bone remodeling
regulation of behavior
negative regulation of synaptic transmission, glutamatergic
cellular response to alkaloid
cellular response to temperature stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support