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CD162 (PSGL-1) Antibody (61-1621-82) in Flow
BALB/c mouse splenocytes were stained with CD3 Monoclonal Antibody, APC (Product # 17-0031-82) and 0.03 µg of Rat IgG1 kappa Isotype Control, PE-eFluor 610 (Product # 61-4301-82) (left) or 0.03 µg of CD162 (PSGL-1) Monoclonal Antibody, PE-eFluor 610 (right). Cells in the lymphocyte gate were used for analysis.
产品信息
61-1621-82
产品规格
种属反应
Mouse
已发表种属
Not Applicable
宿主/亚型
Rat
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
4RA10
抗原
Mouse PSGL-1 human IgG1 fusion protein
偶联物
激发/发射光谱
565/606 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2848496
产品详细信息
Description: This 4RA10 monoclonal antibody reacts with mouse CD162, which is also known as P-selectin glycoprotein ligand-1 (PSGL-1). This clone has been successfully used in flow cytometry and has been reported to block binding of CD162 to CD62P and CD62L.
Applications Reported: This 4RA10 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 4RA10 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PE-eFluor 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red). Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
靶标信息
CD162 (PSGL-1) is a 120 kDa homodimer protein found on the surface of leukocytes and platelets. CD162 is known to bind CD62P (P-selectin), CD62L (L-selectin), and to a lower degree CD62E (E-selectin). These interactions play a critical role in leukocyte adhesion, rolling and extravasation. CD162 has been shown to be an important immune marker of T cell exhaustion in chronic viral infections and cancer.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Cutaneous lymphocyte associated antigen; P-selectin glycoprotein ligand 1; P-selectin glycoprotein ligand-1; selectin P ligand; SELPG
基因别名: CD162; Psgl-1; Psgl1; Selp1; Selpl
Entrez Gene ID: (Mouse) 20345
