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B7-H6 Antibody (62-6526-42) in Flow
HeLa cells were stained with Mouse IgG1 kappa Isotype Control, Super Bright 436 (Product # 62-4714-82) (blue) or B7-H6 Monoclonal Antibody, Super Bright 436 (purple). Total viable cells, as determined by Fixable Viability Dye eFluor 660 (Product # 65-0864-18), were used for analysis.
产品信息
62-6526-42
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
JAM1EW
抗原
B7-H6 recombinant protein
偶联物
激发/发射光谱
413/431 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with BSA
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2784839
产品详细信息
This JAM1EW monoclonal antibody recognizes human B7-H6 (NCR3LG1) protein on the surface of cells. It recognizes protein on live but not on paraformaldehyde-fixed cells. The JAM1EW clone is specific to B7-H6. When tested in flow cytometry against cells transfected with either B7-H4, B7-H5, B7-H6, or B7-H7, clone JAM1EW only stains positively on B7-H6 transfected cells.
Applications Reported: This JAM1EW antibody has been reported for use in flow cytometric analysis.
Applications Tested: This JAM1EW antibody has been pre-diluted and tested by flow cytometric analysis of the HL-60 cell line. This may be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
靶标信息
B7-H6 (NCR3LG1) is a transmembrane endogenous ligand expressed on the surfaces of tumor cells. B7-H6 binds with NKp30 present on NK cells. Binding of B7-H6 to NKp30 causes the ligation of NKp30 subsequently inducing NK cell activation and target cell cytolysis. B7-H6 is not constitutively expressed on normal tissue. Whether it can be expressed endogenously under certain conditions is under investigation. B7-H6 has been detected on circulating pro-inflammatory CD14(+)CD16(+) monocytes in some patients with sepsis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: B7 homolog 6; B7-H6; DKFZp686I21167; Natural cytotoxicity triggering receptor 3 ligand 1; putative Ig-like domain-containing protein DKFZp686O24166/DKFZp686I21167
基因别名: B7-H6; B7H6; DKFZp686O24166; NCR3LG1
UniProt ID: (Human) Q68D85
Entrez Gene ID: (Human) 374383
