Invitrogen

SOD1 Recombinant Superclonal™ Antibody

SOD1 Antibody in Immunocytochemistry (ICC/IF)

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SOD1 Antibody (711818) in ICC/IF

Immunofluorescence of SOD1 was performed using HeLa wild-type and SOD1 KO cells that were transfected with a green or a far red fluorescent dye, respectively. Post-transfection, WT and KO cells were mixed and plated to a 1:1 ratio on coverslips as a mosaic and incubated for 24 hrs. Cells were fixed in 4% PFA (in PBS) for 15 min; cells were permeabilized with 0.1% Triton X-100 for 10 min at RT and blocked with PBS with 5% BSA, 5% goat serum, and 0.01% Triton X-100 for 30 min. Cells were stained with the SOD1 Recombinant Rabbit Superclonal™ ... View More

SOD1 Antibody in Immunoprecipitation (IP)

SOD1 Antibody in Western Blot, Immunoprecipitation (WB, IP)

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SOD1 Antibody — Antibody specificity was demonstrated by siRNA-mediated knockdown of target protein. HeLa cells were transfected with SOD1 specific siRNA and decrease in signal intensity was observed in immunofluorescence application using Anti-SOD1 Recombinant Rabbit Superclonal™ Antibody (Product # 711818). {KD}

产品信息

711818

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

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免疫细胞化学 (ICC/IF)

2 µg/mL

免疫沉淀 (IP)

Assay-dependent

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产品规格

种属反应

Human

已发表种属

Not Applicable

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Superclonal

类型

Antibody

抗原

Protein corresponding to human SOD1 (aa1-aa154)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2688303

产品详细信息

This antibody is predicted to react with Monkey, Mouse, and Rat

Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

靶标信息

SOD1 (superoxide dismutase) binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Identification of high-performing antibodies for Superoxide dismutase [Cu-Zn] 1 (SOD1) for use in Western blot, immunoprecipitation, and immunofluorescence.

F1000Research

Ayoubi R,Alshafie W,You Z,Southern K,McPherson PS,Laflamme C

Published figure using SOD1 recombinant polyclonal antibody (Product # 711818) in Immunoprecipitation

Mon Oct 23 00:00:00 UTC 2023

Identification of high-performing antibodies for Superoxide dismutase [Cu-Zn] 1 (SOD1) for use in Western blot, immunoprecipitation, and immunofluorescence.

F1000Research

Ayoubi R,Alshafie W,You Z,Southern K,McPherson PS,Laflamme C

Published figure using SOD1 recombinant polyclonal antibody (Product # 711818) in Immunoprecipitation

Mon Oct 23 00:00:00 UTC 2023

生物信息学

蛋白别名: Amyotrophic lateral sclerosis 1 (ALS1); Cu/Zn SOD; Cu/Zn superoxide dismutase; epididymis secretory protein Li 44; HGNC:11179; hSod1; indophenoloxidase A; Indophenoloxidase A (IPOA); SOD, soluble; superoxide dismutase; Superoxide dismutase 1; Superoxide Dismutase 1 (SOD1); Superoxide dismutase [Cu-Zn]; superoxide dismutase, cystolic

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基因别名: ALS; ALS1; HEL-S-44; homodimer; hSod1; IPOA; SOD; SOD1

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UniProt ID: (Human) P00441

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Entrez Gene ID: (Human) 6647

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功能