Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 5
Arginase 1 Antibody (754-9779-94) in IHC (P)
Immunohistochemical analysis of Arginase 1 was performed on formalin-fixed paraffin-embedded human liver (positive) and human tonsil (negative) tissues. To expose the target protein, HIER was performed on de-paraffinized sections using BOND Epitope Retrieval Solution 1 (pH 6) for 15 min, followed by a 5 min cool down, a 1 min wash with ddH2O, and then transferred to 1X PBS. Tissues were permeabilized with 0... View More
产品信息
754-9779-94
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
sl6arg
偶联物
激发/发射光谱
558/572 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with 0.5% BSA, 10% proprietary stabilizer
内含物
0.05% sodium azide
保存条件
4° C, store in dark
运输条件
Wet ice
产品详细信息
Description: The monoclonal antibody sl6arg recognizes human Arginase-1, a 35 kDa enzyme that that helps eliminate nitrogen by converting L-arginine to urea and L-ornithine which further converts to polyamine. These polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. Expression is found in the liver, neutrophils, as well as myeloid derived suppressor cells (MDSC) and neural stem cells. Expression in macrophages has not been determined in humans, while in mice expression in M2 macrophages is well established. Arginase-1 may be expressed in the myeloid population in breast cancer tumors and is typically found in the majority of hepatocellular carcinomas.
Applications Reported: This sl6arg antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, immunohistochemical staining, and immunocytochemistry.
Applications Tested: This sl6arg antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue and can be used at 2 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
靶标信息
Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, which is the final step in the urea cycle. The resulting polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. By degrading arginine, Arginase 1 deprives NO synthase of its substrate and down-regulates nitric oxide production. In both human and mouse, Arginase 1 is expressed in the liver, neutrophils, myeloid derived suppressor cells (MDSC) and neural stem cells. In human, expression in blood neutrophils but not in CCR3+ granulocytes has been reported. In mice, expression of Arginase 1 is one of the hallmarks of alternatively activated macrophages (M2a). Arginase-1 may be expressed in the myeloid cells infiltrating tumors, and is typically found in the majority of hepatocellular carcinomas. Defects in Arginase 1 are the cause of argininemia, an autosomal recessive disorder characterized by hyperammonemia.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: A-I; Arginase; arginase 1 liver; arginase, liver; Arginase-1; Arginase1; HGNC:663; Liver Arginase; Liver-type arginase; Type 1 Arginase; Type I arginase
基因别名: ARG1
UniProt ID: (Human) P05089
Entrez Gene ID: (Human) 383
urea cycle
liver development
positive regulation of endothelial cell proliferation
arginine catabolic process
aging
response to herbicide
response to manganese ion
response to zinc ion
response to selenium ion
regulation of L-arginine import
response to amine
arginine catabolic process to ornithine
lung development
collagen biosynthetic process
response to vitamin A
response to vitamin E
response to drug
response to amino acid
response to cadmium ion
response to axon injury
response to methylmercury
mammary gland involution
maternal process involved in female pregnancy
protein homotrimerization
cellular response to hydrogen peroxide
cellular response to lipopolysaccharide
cellular response to interleukin-4
cellular response to glucagon stimulus
cellular response to dexamethasone stimulus
cellular response to transforming growth factor beta stimulus
