Invitrogen

CD4 Monoclonal Antibody (N1UG0), Alexa Fluor™ Plus 750

CD4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CD4 Antibody (757-2444-94) in IHC (P)

Immunohistochemical analysis of CD4 was performed on formalin-fixed paraffin-embedded human tonsil (positive) and human brain (negative) tissues. To expose the target protein, HIER was performed on de-paraffinized sections using BOND Epitope Retrieval Solution 2 (pH 9) for 30 min, followed by a 5 min cool down and a 1 min wash with ddH2O, and then transferred to 1X PBS. Tissues were permeabilized with 0.1% ... View More

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CD4 Antibody — Antibody specificity was demonstrated by the relative expression of target protein in different tissues by IHC. Differential expression of CD4 in human tonsil and human brain tissues using CD4 Monoclonal Antibody (N1UG0), Alexa Fluor™ Plus 750 (Product # 757-2444-94) shows strong staining of target protein in human tonsil tissue and no staining in human brain tissue. {RE}

产品信息

757-2444-94

应用

建议稀释比

已发表文章

免疫组化（石蜡） (IHC (P))

20 µg/mL

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

N1UG0

偶联物

Alexa Fluor™ Plus 750 Alexa Fluor™ Plus 750 Alexa Fluor™ Plus 750

激发/发射光谱

760/788 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.5% BSA, 10% proprietary stabilizer

内含物

0.05% sodium azide

保存条件

4° C, store in dark

运输条件

Wet ice

产品详细信息

Description: The N1UG0 monoclonal antibody reacts with human CD4, a 59 kDa cell surface glycoprotein expressed by the majority of thymocytes, a subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). The N1UG0 antibody is recommended for use in staining human formalin-fixed paraffin embedded tissue sections.

Applications Reported: This N1UG0 antibody has been reported for use in flow cytometric analysis, western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.

Applications Tested: This N1UG0 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using high pH antigen retrieval and can be used at 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

靶标信息

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

仅用于科研。不用于诊断过程。未经明确授权不得转售。