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KLRG1 Antibody (79-5893-82) in Flow
C57BL/6 mouse splenocytes were stained with NK1.1 Monoclonal Antibody, APC (Product # 17-5941-82) and 0.25 µg of Syrian Hamster IgG Isotype Control, Pacific Orange (Product # 79-4914-81) (left) or 0.25 µg of KLRG1 Monoclonal Antibody, Pacific Orange (right). Cells in the lymphocyte gate were used for analysis.
产品信息
79-5893-82
产品规格
种属反应
Mouse
已发表种属
Not Applicable
宿主/亚型
Syrian hamster
/ IgG
分类
Monoclonal
类型
Antibody
克隆号
2F1
偶联物
激发/发射光谱
400/551 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2815359
产品详细信息
Description: This 2F1 monoclonal antibody reacts with the mouse Killer cell Lectin-like Receptor G1 (KLRG1), also known as Mast cell Function-associated Antigen (MAFA). KLRG1 is a homodimer of glycosylated 30-38 kDa subunits and contains a cytoplasmic motif similar to the immunoreceptor tyrosine-based inhibitory motif (ITIM). Rat MAFA was identified as an antigen specific to rat mast cells; however, the expression of mouse KLRG1/MAFA using 2F1 has not been detected on the surface of mouse mast cell lines, bone marrow-derived mast cells, or peritoneal mast cells. This antigen is expressed on approximately one-third of mouse NK cells and a subset of T cells. MHC class I molecules regulate KLRG1 via interactions with class I-specific inhibitory Ly49 molecules and SHP-1 signaling. Although KLRG1 and Ly49 are both lectin-like inhibitory receptors that are regulated by class I MHC expression, the effects of this on cell surface expression of these molecules are opposing, and the underlying regulatory mechanisms distinct.
Applications Reported: This 2F1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 2F1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Pacific Orange emits at 551 nm and is excited with the violet laser line (405 nm). Please make sure that your instrument is capable of detecting this fluorochrome.
Excitation: 405 nm; Emission: 551 nm; Laser: Violet Laser
靶标信息
KLRG1 (Killer cell lectin-like receptor G1, MAFA, 2F1-Ag) is a homodimeric member of the lectin-like type 2 transmembrane receptor family whose members contain characteristic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in their cytoplasmic domains. These ITIMs interact with the SH2 domains of protein phosphatases such as SHP-1. KLRG1 is an inhibitory receptor that is expressed on natural killer (NK) cells and certain T cells. NK cells are involved in the lysis of tumor cells and virus-infected cells, and mediate humoral and cell-mediated immune responses.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Killer cell lectin-like receptor subfamily G member 1; Mast cell function-associated antigen 2F1; natural killer cell receptor
基因别名: 2F1-Ag; Klrg1; MAFA; MAFA-L
UniProt ID: (Mouse) O88713
Entrez Gene ID: (Mouse) 50928
