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ACADM Antibody (PA5-119675) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of of ACADM in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with ACADM Polyclonal Antibody (Product # PA5-119675) at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).
Product Details
PA5-119675
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein within Human ACADM aa 13-239
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2913248
Product Specific Information
Positive Control: Daudi cell lysate, K562 cell lysate, SH-SY5Y, human liver carcinoma tissue, mouse kidney tissue, Hela.
Subcellular Location: Mitochondrion matrix.
Target Information
This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: acyl-CoA dehydrogenase C-4 to C-12 straight chain; acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain; FLJ18227; FLJ93013; FLJ99884; MCAD; MCADH; Medium chain acyl-CoA dehydrogenase; Medium-chain specific acyl-CoA dehydrogenase, mitochondrial; RP4-682C21.1; testicular tissue protein Li 7
Gene Aliases: ACAD1; ACADM; AU018656; MCAD; MCADH
UniProt ID: (Human) P11310, (Mouse) P45952
Entrez Gene ID: (Human) 34, (Mouse) 11364
fatty-acyl-CoA binding
acyl-CoA dehydrogenase activity
electron carrier activity
identical protein binding
flavin adenine dinucleotide binding
oxidoreductase activity, acting on the CH-CH group of donors, with a flavin as acceptor
medium-chain-acyl-CoA dehydrogenase activity
oxidoreductase activity
oxidoreductase activity, acting on the CH-CH group of donors
isomerase activity
dehydrogenase
fatty acid beta-oxidation
carnitine metabolic process, CoA-linked
fatty acid beta-oxidation using acyl-CoA dehydrogenase
carnitine biosynthetic process
medium-chain fatty acid metabolic process
medium-chain fatty acid catabolic process
lipid homeostasis
oxidation-reduction process
liver development
glycogen biosynthetic process
organic acid metabolic process
regulation of gluconeogenesis
lipid metabolic process
fatty acid metabolic process
heart development
metabolic process
response to cold
carnitine metabolic process
post-embryonic development
response to starvation
protein homotetramerization
cardiac muscle cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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