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ACAT1 Antibody (PA5-34676) in ICC/IF
ACAT1 Polyclonal Antibody detects ACAT1 protein at mitochondria by immunofluorescent analysis. Sample: A431 cells were fixed in 2% paraformaldehyde/culture medium at 37°C for 30 min. Green: ACAT1 protein stained by ACAT1 Polyclonal Antibody (Product # PA5-34676) diluted at 1:500. Blue: Hoechst 33342 staining.
Product Details
PA5-34676
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 151 of ACAT1 (Uniprot ID#P24752)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2552028
Product Specific Information
Recommended positive controls: 293T, A431, Molt-4, mouse brain, F31, F90.
Predicted reactivity: Mouse (84%), Rat (83%), Zebrafish (80%), Rhesus Monkey (98%), Bovine (95%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ACAT-1 is a ~50 kDa protein that catalyzes the formation of intracellular cholesterol esters in various tissues, such as adult human liver, adrenal glands, macrophages, and kidneys.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: acetoacetyl Coenzyme A thiolase; Acetoacetyl-CoA thiolase; Acetyl-CoA acetyltransferase, mitochondrial; acetyl-Coenzyme A acetyltransferase 1; mitochondrial acetoacetyl-CoA thiolase; T2; testicular tissue protein Li 198
Gene Aliases: 6330585C21Rik; ACAT; ACAT1; MAT; T2; THIL
UniProt ID: (Human) P24752, (Mouse) Q8QZT1
Entrez Gene ID: (Human) 38, (Mouse) 110446
acetyl-CoA C-acetyltransferase activity
enzyme binding
protein homodimerization activity
metal ion binding
coenzyme binding
catalytic activity
transferase activity
transferase activity, transferring acyl groups
transferase activity, transferring acyl groups other than amino-acyl groups
acyltransferase
liver development
brain development
branched-chain amino acid catabolic process
response to hormone
response to organic cyclic compound
response to starvation
ketone body biosynthetic process
ketone body catabolic process
protein homooligomerization
adipose tissue development
metanephric proximal convoluted tubule development
metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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